Human Interleukin 6,IL-6 ELISA KIT

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Name: Human Interleukin 6,IL-6 ELISA KIT
Brand: CUSABIO
SKU: CSB-E04638h-IS

Code: CSB-E04638h-IS

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, cell culture supernates, tissue homogenates, saliva, urine

Detect Range: 4.7pg/mL- 300pg/mL

Sensitivity: 2.35pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Alternative Names

Interleukin BSF 2 ELISA Kit; B cell differentiation factor ELISA Kit; B cell stimulatory factor 2 ELISA Kit; B-cell stimulatory factor 2 ELISA Kit; BSF 2 ELISA Kit; BSF-2 ELISA Kit; BSF2 ELISA Kit; CDF ELISA Kit; CTL differentiation factor ELISA Kit; Cytotoxic T cell differentiation factor ELISA Kit; Hepatocyte stimulating factor ELISA Kit; Hepatocyte stimulatory factor ELISA Kit; HGF ELISA Kit; HSF ELISA Kit; Hybridoma growth factor ELISA Kit; Hybridoma growth factor Interferon beta-2 ELISA Kit; Hybridoma plasmacytoma growth factor ELISA Kit; IFN-beta-2 ELISA Kit; IFNB2 ELISA Kit; IL 6 ELISA Kit; IL-6 ELISA Kit; IL6 ELISA Kit; IL6_HUMAN ELISA Kit; Interferon beta 2 ELISA Kit; Interferon beta-2 ELISA Kit; Interleukin 6 ELISA Kit; Interleukin 6 (interferon beta 2) ELISA Kit; Interleukin BSF 2 ELISA Kit; Interleukin-6 ELISA Kit

Abbreviation

IL6

Uniprot NO.

P05231

Species

Homo sapiens (Human)

Sample Type

serum, plasma, cell culture supernates, tissue homogenates, saliva, urine

Detection Range

4.7pg/mL- 300pg/mL

Sensitivity

2.35pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-91
1:2	Average %	98
	Range %	91-104
1:4	Average %	90
	Range %	86-106
1:8	Average %	93
	Range %	85-98

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

300

3.031

2.934

2.983

2.878

150

2.574

2.427

2.500

2.396

1.698

1.728

1.713

1.608

37.5

1.050

1.029

1.039

0.935

18.75

0.532

0.511

0.521

0.417

9.4

0.360

0.339

0.349

0.245

4.7

0.199

0.188

0.193

0.089

0.104

0.105

0.104

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This ELISA kit provides quantitative measurement of interleukin-6 (IL-6) in human samples, supporting research into this pleiotropic cytokine's central role in inflammatory signaling, acute-phase responses, and immune dysregulation. Because IL-6 levels can span orders of magnitude between healthy baseline and active disease states such as sepsis or cytokine storm, the kit is designed to capture both constitutive and induced expression across diverse biological compartments.

Detection Range: The 4.7–300 pg/mL range covers basal circulating IL-6 in healthy serum (typically 1–5 pg/mL with dilution consideration) through the elevated concentrations seen in chronic inflammation, autoimmune flares, and post-surgical responses, minimizing the need for extensive sample dilution in most clinical and in vitro stimulation experiments.

Sensitivity: At 2.35 pg/mL, the kit detects the low-picogram concentrations characteristic of unstimulated peripheral blood, early infection, or conditioned media from resting monocytes and fibroblasts where IL-6 secretion is minimal.

Sample Compatibility: Validation across serum, plasma, cell culture supernatants, tissue homogenates, saliva, and urine enables researchers to correlate systemic IL-6 levels with local tissue production or mucosal secretion within a single study, supporting translational and compartment-specific analyses.

Assay Efficiency: A 50–100 µL sample requirement and 1–5 hour protocol accommodate limited-volume specimens from pediatric cohorts or primary cell cultures while supporting same-day data acquisition in high-throughput screening.

This kit is particularly suited for studies in sepsis biomarker profiling, rheumatoid arthritis monitoring, and CAR-T-associated cytokine release syndrome research.

■ Customer Reviews

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Role of the alpha-1 antitrypsin in the progression and severity of COVID-19 infection among Saudi patients RA Ali, WA Mohammedsaeed, MHA Fakher,/,2025

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway (Probable). The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells (Probable).; IL6 is a potent inducer of the acute phase response. Rapid production of IL6 contributes to host defense during infection and tissue injury, but excessive IL6 synthesis is involved in disease pathology. In the innate immune response, is synthesized by myeloid cells, such as macrophages and dendritic cells, upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection or tissue injury (Probable). In the adaptive immune response, is required for the differentiation of B cells into immunoglobulin-secreting cells. Plays a major role in the differentiation of CD4(+) T cell subsets. Essential factor for the development of T follicular helper (Tfh) cells that are required for the induction of germinal-center formation. Required to drive naive CD4(+) T cells to the Th17 lineage. Also required for proliferation of myeloma cells and the survival of plasmablast cells.; Acts as an essential factor in bone homeostasis and on vessels directly or indirectly by induction of VEGF, resulting in increased angiogenesis activity and vascular permeability. Induces, through 'trans-signaling' and synergistically with IL1B and TNF, the production of VEGF. Involved in metabolic controls, is discharged into the bloodstream after muscle contraction increasing lipolysis and improving insulin resistance. 'Trans-signaling' in central nervous system also regulates energy and glucose homeostasis. Mediates, through GLP-1, crosstalk between insulin-sensitive tissues, intestinal L cells and pancreatic islets to adapt to changes in insulin demand. Also acts as a myokine (Probable). Plays a protective role during liver injury, being required for maintenance of tissue regeneration. Also has a pivotal role in iron metabolism by regulating HAMP/hepcidin expression upon inflammation or bacterial infection. Through activation of IL6ST-YAP-NOTCH pathway, induces inflammation-induced epithelial regeneration.

Gene References into Functions

Acute exercise in children with juvenile idiopathic arthritis induced slightly musculoskeletal leg pain and transient increased plasma calprotectin levels but not IL-6 levels PMID:30008613
Genotype frequencies in the degenerative lumbar scoliosis patients and controls revealed a significant difference for the IL6-572 G/C polymorphism. A significant association was found between the IL6-572 G/C polymorphism and measured bone mineral densities at the lumbar spine. PMID:28378072
In glioblastoma, colony-stimulating factor-1 and angiocrine IL-6 induce robust arginase-1 expression and macrophage alternative activation, mediated through peroxisome proliferator-activated receptor-gamma-dependent transcriptional activation of hypoxia-inducible factor-2alpha. PMID:29422647
study demonstrated novel molecular events for leptin-induced inflammation in ligamentum flavum (LF) tissue by promoting IL-6 expression and thus might have potential implications for clarifying the mechanism underlying LF fibrosis and hypertrophy. PMID:29436483
The objective of this study was to evaluate the diagnostic value of serum and synovial fluid interleukin (IL)-6 levels for Periprosthetic Joint Infection. PMID:28473693
The elevated levels of both serum Shh and IL-6 were mainly observed in BC patients who had a significantly higher risk of early recurrence and bone metastasis, and associated with a worse survival for patients with progressive metastatic BC. PMID:28496132
study suggests that-174 G/C polymorphism of IL-6 gene differs in athletes, G allele and GG genotype is higher than the other ones, at least in Turkish athletes, and therefore should be taken into consideration when determining genetic aspects of athletes. PMID:30213294
studies reveal that IL-6 action in T cells through classical IL-6 signalling promotes inflammation and insulin resistance early during obesity development, which can be compensated for by enhanced IL-6 trans-signalling at later stages PMID:28466852
IL-6 signalling in primary human macrophages increased intracellular Bacillus Calmette-Guerin (BCG) and Mycobacterium tuberculosis numbers in a dose-dependent manner promoting mycobacterial survival and BCG-induced lipid accumulation. PMID:28262681
The analysis of the effect of the individual SNPs(PON1, IL-6, ITGB3, and ALDH2 ) and GRS groups on different lipid profile parameters revealed no significant association of any of the tested SNPs with any lipid parameter, however, the GRS groups showed marginally significant for TC and highly significant association for TG, LDL-c and HDL-c PMID:30261890
Our results showed that IL-37 plays an inhibitory role in non-small cell lung cancer progression, possibly by suppressing STAT3 activation and decreasing epithelial-to-mesenchymal transition by inhibiting IL-6 expression. IL-37 could serve as a potential novel tumor suppressor in non-small cell lung cancer PMID:29575809
Although interleukin-6 (IL-6) mRNA level was higher in 3D-culured cells, its secretion levels were higher in 2D-cultured cells. In addition, the levels of mRNA and protein expression of regnase-1, regulatory RNase of inflammatory cytokine, significantly increased in 3D culture, suggesting post-translational modification of IL-6 mRNA via regnase-1. PMID:30096769
indicate that the distribution of IL6-174G/C (rs1800795) SNP was marginally associated with multiple sclerosis susceptibility PMID:30069682
FABP5 promotes tumor angiogenesis via activation of the IL6/STAT3/VEGFA signaling pathway in hepatocellular carcinoma. PMID:29957468
Authors found that the IL-6 serum level was significantly higher in the SIRS group than in the control group. A significant association was observed in the genotypic distribution of the IL-6 - 572G allele in the SIRS group, when compared with the control group, and SIRS is more likely to occur in wasp sting patients with more than 10 stings. PMID:30265566
adipocytes are capable of enhancing IL-6 production by CD4(+) T cells PMID:29283192
Studied association of levels of IL-6 (interleukin-6) and TGF-beta in the pathogenesis of idiopathic epistaxis. PMID:29893909
LL was significantly negatively correlated with PGC-1alpha, TNF-alpha, and IL-6 mRNA expressions. PGC-1alpha mRNA expression levels in paraspinal muscles may be affected by lumbar kyphosis. PMID:30233161
Study found that IL-6 and IL-8 are necessary and sufficient to increase tumor cell migration in a cell density dependent manner with negligible feedback on cell proliferation. This effect is specific to metastatic cancer cells; IL-6 and IL-8 have no effect on the migration of normal and non-metastatic cancer cells. PMID:28548090
Rheumatoid arthritis patients who had the best response to tocilimuzab had the highest levels of IL6 and the lowest levels of soluble IL6 receptor. PMID:29157669
High IL6 expression is associated with retinopathy of prematurity. PMID:29274846
Adult serum IL-6 levels were predicted across periods as long as 15 years by adolescents' inability to defuse peer aggression and poor peer-rated conflict resolution skills, and by independently observed romantic partner hostility in late adolescence. PMID:29212559
Lysophosphatidylcholine induces COX-2-mediated IL-6 expression. NADPH oxidase/Reactive Oxygen Species is involved in Lysophosphatidylcholine-induced COX-2 expression. PMID:30229288
The expression of IL-6 gene and protein was significantly induced by IL-17F. IL-17F activated TAK1 and NF-kappaB in airway smooth muscle cells. PMID:28474507
IL-6 was over-expressed in SF from OA patients compared with normal donors. DNA hypomethylation and histone hyperacetylation were observed in the IL-6 promoter region in OA SF compared with normal SF. No differences in the status of H3K9 di-methylation, H3K27 tri-methylation and H3K4 tri-methylation in the IL-6 promoter region were observed between normal and OA SF. PMID:28262826
Increased serum IL6 concentrations are associated with obstructive sleep apnea and glycemic status. PMID:29305826
Study showed that activation of NF-kappaB/IL-6 is involved in moderate hyperthermia treatment induced progression of hepatocellular carcinoma cells. PMID:29894725
the polymorphism rs1800795 is associated with serum IL-6 level and level of neuroblastoma risk; GG genotype might indicate that the tumor is highly malignant (prone to metastasis) and associated with poor prognosis PMID:29692379
Findings indicate that childhood infections do not have an independent, lasting effect on circulating inflammatory marker levels subsequently in childhood; however, elevated inflammatory markers may be harmful for intellectual development/function. PMID:29198208
This study found that the protein and mRNA expression levels of the IL-6 is significantly increased. PMID:28476335
IL-6 may be used as a tumor marker for cancer diagnosis. It may be a clinically significant predictor and may represent a target for cancer treatment. However, to definitely conclude this, further extensive studies would be required PMID:30249899
The findings suggest that male factor infertility might be associated with an increased level of interleukin-6. PMID:28523952
Individuals with posttraumatic stress disorder showed a significant increase in the serum levels of IL-6 (and IL-10). PMID:29179015
Data suggest that, in children with pediatric obesity, lifestyle weight-loss intervention results in down-regulation of serum cardiotrophin-1 (CTF1), interleukin-6 (IL6), and tumor necrosis factor-alpha (TNFA); expression of CTF1, IL6, and TNFA is also down-regulated in peripheral blood mononuclear cells after improvement in adiposity, body mass index, and waist-hip ratio. PMID:28749076
Findings outlined in the current study demonstrated that the inhibition of P16 decreased the growth and metastasis potential of BC cells by inhibiting IL-6/JAK2/STAT3 signaling. PMID:29388151
The G/C genotype and the minor allele C of the IL-6 rs1800795 SNP were more common in individuals with Type 2 Diabetes Mellitus than controls (p = 0.004, odds ratio [OR] = 1.98, 95% confidence interval [CI]: 1.24-3.18 and p = 0.011, OR = 1.59, 95% CI: 1.11-2.26, respectively). PMID:29957071
The C allele of rs1800795 within IL-6 gene promoter, rs1800795-tobacco smoking and rs1800795-alcohol drinking interaction were all associated with increased CAD risk. PMID:29889576
study emphasizes the importance of -572G > C polymorphism in increasing IL-6 levels, thereby showing its significant role in DVT in India. PMID:29890913
Interleukin-6 Single Nucleotide Polymorphism is associated with Prostate Adenocarcinoma and Bone Metastasis. PMID:29938471
In a multi-ethnic population with nonalcoholic fatty liver disease, IL-6 is independently associated with the prevalence and severity of subclinical coronary atherosclerosis. PMID:29579601
in the patients with primary depression, depressive symptoms were associated with IL-6 PMID:30148175
eNOS knockdown greatly enhanced endothelial IL-6 production and permeability in response to LPS. Knockdown of eNOS enhanced LPS-induced p38. Inhibition of p38 with SB203580 prevented IL-6 production, without altering permeability PMID:29061842
The expression of the inflammatory cytokines interleukin (IL)6 and IL8 was significantly increased in endometriotic and cocultured cells compared with healthy ECs. PMID:29901132
Leptin-to-adiponectin ratio and IL-6 were elevated in men with prostate cancer. Leptin, chemerin and IL-6 were associated with Gleason score. The relationships between leptin, chemerin and IL-6 were dependent on each other. PMID:29465157
Collective evidence is supportive of the idea that IL-6 is an important participant during the EMT process in human intrahepatic biliary epithelial cells (HIBECs), as IL-6 stimulation can enhance the migration abilities of HIBEC, promote HIBEC cellular senescence and inhibit apoptosis of HIBECs, resulting in the EMT transformation of HIBECs. PMID:28857276
Our findings suggest that IL-6-mediated cross-talk between preadipocytes and breast DCIS cells can promote the progression of early stage breast cancer. PMID:30134951
Our study suggests the second day as the golden time for measuring the serum levels of IL-6. These findings warn us to take more health care actions in patients with higher serum levels of IL-6 on the second day. PMID:29947344
A small drug acting as a JAK1/2 inhibitor may also counteract the repressing effects of IL-6. PMID:29162613
addition of colivelin, a STAT3 activator, instead of IL-6 and C2C12 conditioned medium, promoted the myogenic differentiation of adipose tissue-derived stem cells. PMID:29882916
IL-6 G allele promoter increased stroke recurrent risk, therefore, it would be a predictor for recurrence of stroke in the young with moderate internal carotid artery stenosis. PMID:29091301