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Human Interleukin-22 receptor subunit alpha-2(IL22RA2) ELISA kit

Human Interleukin-22 receptor subunit alpha-2(IL22RA2) ELISA kit

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Code: CSB-EL011637HU

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 31.25 pg/mL-2000 pg/mL

Sensitivity: 7.81 pg/mL

Trial Size:

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Target Background

Product Details

Alternative Names

IL 22BP ELISA Kit; IL 22RA2 ELISA Kit; IL22RA2 ELISA Kit; Class II cytokine receptor ELISA Kit; CRF2 10 ELISA Kit; CRF2 S1 ELISA Kit; CRF2-10 ELISA Kit; CRF2-S1 ELISA Kit; CRF2X ELISA Kit; Cytokine receptor class II member 10 ELISA Kit; Cytokine receptor class-II member 10 ELISA Kit; Cytokine receptor family 2 member 10 ELISA Kit; Cytokine receptor family type 2 ELISA Kit; Cytokine receptor family type 2 soluble 1 ELISA Kit; I22R2_HUMAN ELISA Kit; IL 22 receptor subunit alpha 2 ELISA Kit; IL 22R alpha 2 ELISA Kit; IL-22 receptor subunit alpha-2 ELISA Kit; IL-22BP ELISA Kit; IL-22R-alpha-2 ELISA Kit; IL-22RA2 ELISA Kit; IL22 RA2 ELISA Kit; IL22BP ELISA Kit; IL22R alpha 2 ELISA Kit; Il22ra2 ELISA Kit; Interleukin 22 binding protein ELISA Kit; Interleukin 22 Receptor alpha 2 ELISA Kit; Interleukin-22 receptor subunit alpha-2 ELISA Kit; Interleukin-22-binding protein ELISA Kit; MGC150509 ELISA Kit; MGC150510 ELISA Kit; soluble 1 ELISA Kit; ZcytoR16 ELISA Kit

Abbreviation

IL22RA2

Uniprot NO.

Q969J5

Species

Homo sapiens (Human)

Sample Type

serum, plasma, tissue homogenates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.81 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IL22RA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	87-98
1:2	Average %	107
	Range %	103-112
1:4	Average %	91
	Range %	85-97
1:8	Average %	92
	Range %	88-96

Recovery

The recovery of human IL22RA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	98-105
EDTA plasma (n=4)	98	93-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	1.927	2.057	1.992	1.889
1000	1.342	1.460	1.401	1.298
500	0.922	0.965	0.944	0.841
250	0.651	0.663	0.657	0.554
125	0.432	0.412	0.422	0.319
62.5	0.254	0.269	0.262	0.159
31.25	0.208	0.201	0.205	0.102
0	0.104	0.102	0.103

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Human Interleukin-22 receptor subunit alpha-2(IL22RA2) ELISA kit

Description

This Human IL22RA2 ELISA Kit was designed for the quantitative measurement of Human IL22RA2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

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Target Background

Function(From Uniprot)

Isoform 2 is a receptor for IL22. Binds to IL22, prevents interaction with the functional IL-22R complex and blocks the activity of IL22 (in vitro). May play an important role as an IL22 antagonist in the regulation of inflammatory responses.; Isoform 1 may play a role in establishing and maintaining successful pregnancy.

Gene References into Functions

expression higher in the epidermal compartment of the skin in comparison with dermis in both healthy skin and psoriasis skin PMID:27676439
suggest that the human IL-22BP isoforms have distinct spatial and temporal roles and coordinately fine-tune IL-22-dependent STAT3 responses in tissues as a type of rheostat. PMID:27678220
thia study shows shows that nonaffected skin of psoriasis patients displays lower expression of IL-22BP than skin of healthy controls, and that the strong IL-22 increase in lesional psoriatic skin is accompanied by a moderate induction of IL-22BP PMID:28356382
The deletion of IFNGR1 causes complete IFN-gammaR1 deficiency. Despite the deletion ending very close to the IL22RA2 gene, it does not appear to affect IL22RA2 transcription. PMID:26931784
IL-22BP is produced by eosinophils in the gut and blocks IL-22 protective actions in colitis. PMID:26329427
Through a combined approach including genetic and immunological investigation in an animal model and large-scale association studies of patients with multiple sclerosis (MS), IL-22RA2 is established as an MS risk gene. PMID:21041731
The IL-22R and IL-10R2 binding sites are juxtaposed on adjacent IL-22 surfaces contributed mostly by helices A, D, and F and loop AB. PMID:18675824
Crystallization and preliminary X-ray diffraction results of the IL-22-IL-22 binding protein (IL-22BP) complex are described. PMID:19193995
Polymorphisms in IL22RA1 are associated with severe CRS. PMID:19393422

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Subcellular Location

Secreted.

Protein Families

Type II cytokine receptor family

Tissue Specificity

Expressed in placenta, spleen, breast, skin and lung. Also detected in intestinal tract, testis, brain, heart and thymus. No expression found in prostate, bladder, kidney, ovary, muscle, bone marrow, liver and uterus. Isoform 1 is expressed only in placen

Database Links

HGNC: 14901

UNIGENE: Hs.126891

KEGG: hsa:116379

STRING: 9606.ENSP00000296980

OMIM: 606648

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