Human Epithelial neutrophil activating peptide 78 (ENA-78/CXCL5) ELISA Kit

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Human Epithelial neutrophil activating peptide 78 (ENA-78/CXCL5) ELISA Kit

Name: Human Epithelial neutrophil activating peptide 78 (ENA-78/CXCL5) ELISA Kit
Brand: CUSABIO
SKU: CSB-E08178h-IS

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Code: CSB-E08178h-IS

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 47 pg/mL-3000 pg/mL

Sensitivity: 23.5 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Alternative Names

AMCFII ELISA Kit; C-X-C motif chemokine 5 ELISA Kit; C-X-C motif chemokine ligand 5 ELISA Kit; Chemokine (C X C motif) ligand 5 ELISA Kit; chemokine (C-X-C motif) ligand 5 ELISA Kit; Cxcl5 ELISA Kit; CXCL5_HUMAN ELISA Kit; ENA 78 ELISA Kit; ENA-78 (8-78) ELISA Kit; ENA-78(1-78) ELISA Kit; ENA-78(9-78) ELISA Kit; ENA78 ELISA Kit; Epithelial derived neutrophil activating protein 78 ELISA Kit; Epithelial-derived neutrophil-activating protein 78 ELISA Kit; Lipopolysaccharide-induced CXC chemokine ELISA Kit; Neutrophil activating peptide ENA 78 ELISA Kit; Neutrophil activating protein 78 ELISA Kit; Neutrophil-activating peptide ENA-78 ELISA Kit; neutrophil-activating protein 78 ELISA Kit; SCYB5 ELISA Kit; Small inducible cytokine B5 ELISA Kit; small inducible cytokine subfamily B (Cys-X-Cys); member 5 (epithelial-derived neutrophil-activating peptide 78) ELISA Kit; small inducible cytokine subfamily B; member 5 ELISA Kit; Small-inducible cytokine B5 ELISA Kit

Abbreviation

CXCL5

Uniprot NO.

P42830

Species

Homo sapiens (Human)

Sample Type

serum, plasma, tissue homogenates

Detection Range

47 pg/mL-3000 pg/mL

Sensitivity

23.5 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ENA-78/CXCL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	99
	Range %	92-106
1:2	Average %	95
	Range %	88-102
1:4	Average %	89
	Range %	84-95
1:8	Average %	92
	Range %	85-99

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

3000

3.093

2.934

3.013

2.831

1500

2.440

2.567

2.504

2.322

750

1.797

1.828

1.813

1.630

375

1.248

1.312

1.280

1.098

187.5

0.604

0.621

0.613

0.430

0.386

0.373

0.380

0.198

0.292

0.284

0.288

0.106

0.179

0.185

0.182

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This ELISA kit provides quantitative measurement of CXCL5 (ENA-78) in human samples, supporting research into neutrophil recruitment and CXC chemokine signaling in inflammatory and tumor microenvironments. Because CXCL5 levels can vary dramatically between healthy baseline and active inflammatory or tumorigenic states, the kit is designed to capture this biologically meaningful dynamic range without excessive sample manipulation.

Detection Range: The 47–3000 pg/mL range spans from low baseline circulating CXCL5 concentrations in healthy plasma to the elevated levels observed in sepsis, COPD exacerbations, and tumor-associated angiogenesis, minimizing the need for serial dilutions in most inflammatory disease samples.

Sensitivity: At 23.5 pg/mL, the kit detects CXCL5 in conditions where expression is modest, such as early-stage tumor stroma or resting endothelial cell supernatants, where neutrophil-recruiting chemokine gradients are just forming.

Sample Compatibility: Validation across serum, plasma, and tissue homogenates enables researchers to correlate systemic CXCL5 levels with local tissue expression — critical for studies dissecting neutrophil infiltration into tumor or inflamed tissue compartments.

Assay Efficiency: The 1–5 hour protocol using 50–100 µL accommodates limited clinical specimens, such as serial plasma draws from patients, and small tissue biopsies where homogenate volume is constrained.

This kit is well-suited for studies in tumor-associated neutrophil biology, chronic obstructive pulmonary disease, and obesity-linked adipose tissue inflammation.

■ Customer Reviews

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In Vitro Anti-Aging Effects of Yeast/Rice Fermentation Filtrate Combined with Sialic Acid in Cosmetic Applications F Yang, M Li, Y Zuo, L Zhang, J Wu, Z Liu, H Wang,/,2025

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes.

Gene References into Functions

Serum CXCL5 levels from pemphigus vulgaris patients are significantly higher than those in bullous pemphigoid patients and healthy controls. PMID:27501402
These data demonstrated that CXCL5 expression was upregulated in prostate cancer tissues and that exogenous CXCL5 protein exposure or CXCL5 overexpression promoted malignant phenotypes of prostate cancer cells in vitro and in vivo. PMID:29749439
activated CXCL5-CXCR2 axis contributes to the metastatic phenotype of PTC cells by modulating Akt/GSK-3beta/beta-catenin pathway PMID:29471001
study elucidates the important role of CXCL5 in the progression and prognosis of NSCLC. These findings suggested that CXCL5 might be a potential biomarker and novel therapeutic target for lung cancer PMID:29526026
PERK-p-eIF2alpha pathway could suppress metastasis in triple-negative breast cancer by inhibiting expression of PDL1 and CXCL5 in tumor cells. PMID:29053414
Mechanistically, AR modulated cytokine CXCL5 expression by altering AKT --> NF-kappaB signaling, and interruption of AKT --> NF-kappaB --> CXCL5 signaling using either specific inhibitors or siRNA suppressed AR-enhanced EC recruitment and AR-EC-promoted RCC progression. PMID:27848972
Curcumin suppressed CXCL5 expression by direct inhibition of IKKbeta phosphorylation, and inhibition of p38 MAPK via induction of negative regulator MKP-1. PMID:27538525
The CXCL5 and the overexpression of miR-141 reduced levels of MMP-2 and MMP-9 in tumor necrosis factor-alpha-treated HT29 cells by means of repressing the inhibitory AKT. PMID:28854064
CXCL5 may promote mitomycin resistance by activating EMT and NF-kappaB pathway. Thus, this study identifies CXCL5 as a novel chemoresistance-related marker in non-muscle invasive bladder cancer PMID:29545183
findings for the first time provided evidence that ENA78 may play a key role of mediator in pathogenesis of Major Depressive Disorder(MDD) and in the mechanism of vinlafaxine effects on MDD. PMID:28441588
our findings support CXCL5 as a promoter of colorectal cancer metastasis and a predictor of poor clinical outcomes in colorectal cancer patients. PMID:28356111
CXCL5 levels were decreased in LSCC patient serum. PMID:27876461
a finely tuned balance between the GAG-bound dimer and free soluble monomer regulates CXCL5-mediated receptor signaling and function. PMID:27471273
CXCL5 plays a promoting role in glioma in autocrine- and paracrine-dependent manners. PMID:27748886
The expression of CXCL5 is up-regulated in osteosarcoma cells. PMID:28277189
CXCL5 expression in urine is related to bladder cancer TNM stage, lymph node metastasis, tumor size, and tumor grade. PMID:26503215
ENA 78 plasma levels were correlated with Expanded Disability Status Scale scores in neuromyelitis optica (NMO) patients; elevated secretion of ENA 78 may be a critical step in neutrophil recruitment during the remission of NMO. PMID:27401736
CXCL5 expression is enriched in human atherosclerotic coronary artery. The CXCL5 variant might be a genetic risk factor for the susceptibility of CAD and the CXCL5 promoter -156 G/C C allele might be an independent predictor for CAD. PMID:26287498
Study shows that CXCL5 expression is elevated in positive correlation to bladder cancer grade and promotes cell migration and invasion via binding to its receptor CXCR2. PMID:26058729
Analysis of monocultured dermal fibroblasts and keratinocytes revealed that only fibroblasts but not keratinocytes displayed up regulated CXCL5 levels after UV stimulation. PMID:25690483
There was an inverse correlation between DACH1 mRNA levels and CXCL5 in both lung cancer cell lines and human NSCLC tissues. PMID:25788272
High CXCL5 expression is associated with pediatric ulcerative colitis. PMID:25738378
the increased level of CXCL5 in tissue compartments, including the central nervous system of HIV-1-infected individuals might alter the inflammatory response through the infiltration of neutrophils into tissue compartment PMID:25536401
Serum levels of ENA-78 were elevated in autistic children and they were significantly associated with the increased levels of serum antineuronal auto-antibodies PMID:25871636
This study aims to evaluate serum levels of ENA78/CXCL5 and SDF-1/CXCL12 along the gastric cancer carcinogenesis, and analyze their clinical significance, and diagnostic potentials through human serum samples. PMID:25689618
our data showed that the CXCR2/CXCL5 axis contributes to EMT of HCC cells through activating PI3K/Akt/GSK-3beta/Snail signaling, and it may serve as a potential therapeutic target. PMID:25462858
Solution structure of CXCL5--a novel chemokine and adipokine implicated in inflammation and obesity PMID:24695525
the expression levels of CXCL5 proteins were decreased in dermal blood vessels of early stage diffuse cutaneous systemic sclerosis PMID:24292093
data indicates that LCCs per se may act as the producer and receptor of CXCL5 responsible for liver cancer migration and invasion PMID:25011526
Citrullinated ENA-78/CXCL5 is highly correlated with rheumatoid arthritis disease activity and, unlike noncitrullinated ENA-78/CXCL5, recruits monocytes. PMID:24943990
CXCL5 showed a statistically significant prognostic effect PMID:24500664
mRNA and protein of CXCL5 is increased in bladder tumor tissues and cell lines; down-regulation of CXCL5 resulted in significantly decreased cell proliferation, migration and increased cell apoptosis through Snail, PI3K-AKT and ERK1/2 signaling pathways. PMID:24583128
Knockdown of HSP27 by shRNA decreased HB-EGF plus CXCL5-mediated tumor spheroid formation in a three-dimensional culture system, suggesting that AKT/HSP27 was required for HB-EGF/CXCL5-mediated cancer progression PMID:24346967
Liver cancer cells with high metastatic potential have a higher expression of CXCL5. Exogenous CXCL5 can increase the proliferation, migration and invasion of liver cancer cells with low metastatic potential. PMID:23290114
sCXCL5 level was determined to be an independent prognostic factor for NPC patients PMID:23469080
Increased levels of CXCL5 contribute to enhanced levels of RANKL expression in Paget's disease of bone. PMID:23439434
CXCL5 gene polymorphisms are functional and associated with variable blood pressure in cardiovascular disease-free individuals. PMID:23245743
CXCL5 promotes HCC cell proliferation, invasion, and intratumoral neutrophil infiltration. PMID:22711685
Preoperative serum CXCL5 could serve as a novel predictive marker for prognosis determination of colorectal cancer patients. PMID:22197219
Endothelial production of both ENA-78 and IL-8 was induced by the proinflammatory cytokine IL-1beta. PMID:22274300
Serum CXC ligand 5 is a new marker of subclinical atherosclerosis in type 2 diabetes. PMID:21609350
Blockade of CXCL5 can modulate IL-17-induced arthritic inflammation in part by reducing joint blood vessel formation through a non-overlapping IL-17 mechanism. PMID:21779896
Data demonstrate that the chemokine CXCL5 is a peripheral mediator of UVB-induced inflammatory pain, likely in humans as well as rats. PMID:21734176
Plasma CXCL5 levels are lower in patients with chronic liver disease, suggesting that CXCL5 might be involved in the pathogenesis of chronic liver disease. PMID:21332547
CXCL5/ENA78 increased cell migration and epithelial-to-mesenchymal transition of hormone-independent prostate cancer by early growth response-1/snail signaling pathway. PMID:20945384
Report influence of troglitazone, sodium butyrate, 5-aminosalicylic acid and BAY 11-7082 on the chemokine ENA-78/CXCL5 secretion in the intestinal subepithelial myofibroblasts. PMID:21229889
CD14 and CXCL5 were both expressed in tunica intima and tunica adventitia of adipose tissue blood vessels; CXCL5 exhibited chemoattractant and angiogenic properties. PMID:21034724
The highly divergent effects of modifications of CXCL5 on neutrophil influx underline the potential importance of tissue-specific interactions between chemokines and PAD or proteases. PMID:20630876
expression in gingival epithelial cells is induced by thrombin via activation of protease-activated receptor 1 PMID:19567485
Elevated circulating CXCL5 concentrations were associated with higher risk of hypercholesterolemia in middle-aged and elderly Chinese independent of obesity, inflammation, adipokines, and other risk factors but not insulin resistance. PMID:20501684