Abbreviation
Recombinant Human CXCL5 protein, partial (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration of 5.0-10 ng/ml.
Alternative Names
AMCFII; C-X-C motif chemokine 5; C-X-C motif chemokine ligand 5; Chemokine (C X C motif) ligand 5; chemokine (C-X-C motif) ligand 5; Cxcl5; CXCL5_HUMAN; ENA 78; ENA-78 (8-78); ENA-78(1-78); ENA-78(9-78); ENA78; Epithelial derived neutrophil activating protein 78; Epithelial-derived neutrophil-activating protein 78; Lipopolysaccharide-induced CXC chemokine; Neutrophil activating peptide ENA 78 ; Neutrophil activating protein 78; Neutrophil-activating peptide ENA-78; neutrophil-activating protein 78; SCYB5; Small inducible cytokine B5 ; small inducible cytokine subfamily B (Cys-X-Cys); member 5 (epithelial-derived neutrophil-activating peptide 78); small inducible cytokine subfamily B; member 5; Small-inducible cytokine B5
Species
Homo sapiens (Human)
Expression Region
41-114aa
Target Protein Sequence
AAVLRELRCV CLQTTQGVHP KMISNLQVFA IGPQCSKVEV VASLKNGKEI CLDPEAPFLK KVIQKILDGG NKEN
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CXCL5 (ENA-78) is a potent CXC chemokine that drives neutrophil recruitment through CXCR2 signaling, making it central to studies of acute inflammation and tumor-associated immune infiltration. This tag-free recombinant human CXCL5, spanning residues 41–114 of the mature processed sequence, demonstrates robust chemotactic activity on human peripheral blood neutrophils at 5.0–10 ng/mL, providing a suitable basis for neutrophil migration assays, CXCR2-dependent MAPK and NF-κB signaling studies, and in vivo inflammation models. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that can confound innate immune cell readouts, while purity exceeding 97% by SDS-PAGE supports use as a reliable reference standard in CXCL5 detection ELISAs or as a positive control for antibody validation in western blot. The E. coli-derived, untagged format faithfully represents the native mature chemokine, aligning with standards expected in functional bioassays where receptor-binding fidelity is essential.
