Human Complement 7,C7 ELISA Kit

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Target Background

Name: Human Complement 7,C7 ELISA Kit
Brand: CUSABIO
SKU: CSB-E11167h

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Code: CSB-E11167h

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Size:

96T,5×96T,10×96T

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Sample Types: serum, plasma, tissue homogenates

Detect Range: 15.6 ng/mL-1000 ng/mL

Sensitivity: 3.9 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Target Background

Target Name

complement component 7

Alternative Names

C7 ELISA Kit; CO7_HUMAN ELISA Kit; complement component 7 ELISA Kit; Complement component C7 ELISA Kit

Abbreviation

Uniprot NO.

P10643

Species

Homo sapiens (Human)

Sample Type

serum, plasma, tissue homogenates

Detection Range

15.6 ng/mL-1000 ng/mL

Sensitivity

3.9 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human C7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	91
	Range %	87-95
1:200	Average %	95
	Range %	90-100
1:400	Average %	97
	Range %	94-101
1:800	Average %	94
	Range %	91-98

Recovery

The recovery of human C7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	99	93-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.934	2.880	2.907	2.806
500	2.102	2.195	2.149	2.048
250	1.421	1.504	1.463	1.362
125	0.807	0.772	0.790	0.689
62.5	0.443	0.459	0.451	0.350
31.2	0.269	0.259	0.264	0.163
15.6	0.171	0.167	0.169	0.068
0	0.099	0.103	0.101

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Human Complement 7,C7 ELISA Kit

Description

This Human complement component 7 ELISA Kit was designed for the quantitative measurement of Human complement component 7 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 ng/mL-1000 ng/mL and the sensitivity is 3.9 ng/mL.

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Functional genetic variants in complement component 7 confer susceptibility to gastric cancer S Wang,/,2022

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C7 serves as a membrane anchor.

Gene References into Functions

this study shows that GG genotype of C7 provides protection against fibrosis severity while showing a higher risk for hepatocellular carcinoma in patients with hepatitis C PMID:29966690
Two case reports of C7 deficiency leading to recurrent meningitis and other bacterial infections are described. PMID:28078901
The complement C7 rs6876739 CC genotypes and mannan-binding lectin (MBL2) gene polymorphisms of liver donors were significantly associated with bacterial infection in recipients. PMID:27063552
Complement proteins C7 and CFH control the stemness of liver cancer cells via LSF-1 pathway. PMID:26723877
Borrelial CspA binds the human terminal complement components C7 and C9 and blocks assembly and membrane insertion of the terminal complement complex (TCC). PMID:23943762
Studies indicate that the deletion defect may be a more commonly distributed cause of C7 deficiency in Ireland. PMID:22206826
C7 isoelectric focusing variants can determine meningococcal killing in the early stage of infection when antibody-independent killing prevails. PMID:19931914
To determine transcriptional regulation of the human complement component C7, a 1 kb promoter fragment was cloned and the transcription start site was determined. C7 is expressed by the hepatoma-derived cell line Hep-3B, but not by Hep-G2. PMID:12595902
The interaction between the factor I domain of C7 and the C345C domain at the C terminus of the C5 alpha-chain plays an essential role in complement membrane attack complex formation and complement lytic activity. PMID:15879120
recurrence of fulminant meningococcal disease in a complement component C7-deficient patient PMID:15889368
membrane associated C7 acts as a trap for the late complement components to control excessive inflammation induced by SC5b-9 PMID:19179470
C7 is associated with multiple sclerosis pathogenesis. PMID:19221116
Data show that the two C7-FIMs pack closely together with an approximate 2-fold rotational symmetry that is rarely seen in module pairs and has not been observed in FD-containing proteins. PMID:19419965