Goat Tumor necrosis factor α,TNF-α ELISA KIT

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Target Background

Name: Goat Tumor necrosis factor α,TNF-α ELISA KIT
Brand: CUSABIO
SKU: CSB-E09811g

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Code: CSB-E09811g

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 15.6 pg/mL-1000 pg/mL

Sensitivity: 3.9 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Target Background

Alternative Names

TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a

Abbreviation

TNF

Uniprot NO.

P13296

Species

Capra hircus (Goat)

Sample Type

serum, plasma, tissue homogenates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of goat TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	81-91
1:2	Average %	98
	Range %	91-103
1:4	Average %	99
	Range %	95-104
1:8	Average %	94
	Range %	86-98

Recovery

The recovery of goat TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-97
EDTA plasma (n=4)	97	92-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.601	2.589	2.595	2.493
500	2.154	2.133	2.144	2.042
250	1.658	1.621	1.640	1.538
125	1.123	1.114	1.119	1.017
62.5	0.712	0.665	0.689	0.587
31.2	0.413	0.399	0.406	0.304
15.6	0.256	0.242	0.249	0.147
0	0.104	0.100	0.102

Troubleshooting and FAQs

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ELISA Data Analysis

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Goat Tumor necrosis factor α,TNF-α ELISA KIT

Description

This Goat TNF ELISA Kit was designed for the quantitative measurement of Goat TNF protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

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Th1/Th2 immune responses and oxidative stress in caprine flea allergy dermatitis Ajith Y.et al,/,2017
Lice induced immuno-oxidative wreckage of goats Y. Ajith.et al,/,2017
Rhubarb supplementation promotes intestinal mucosal innate immune homeostasis through modulating intestinal epithelial microbiota in goat kids Jinzhen Jiao.et al,/,2018
Intraarticular injection autologous platelet-rich plasma and boneMarrow concentrate in a goat osteoarthritis model Wang Z.et al,/,2018
Host immunomodulation by ascorbic acid ameliorates oxidative stress in caprine pediculosis—A pilot study Y.Ajith,/,2019
Effect of Varying Levels of Hempseed Meal Supplementation on Humoral and Cell-Mediated Immune Responses of Goats F Abrahamsen,/,2021
Dietary Tea Tree (Melaleuca alternifolia) Oil Supplementation Improves Growth Performance, Cecal Microflora, Immunity, and Antioxidant Capacity of Partridge Shank Chickens X Lv,/,2022

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

Gene References into Functions

Data indicate that three single-nucleotide polymorphisms were identified in tumor necrosis factor-alpha (TNF-alpha) gene. PMID:25966128

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Subcellular Location

Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.

Protein Families

Tumor necrosis factor family

Database Links

UNIGENE: Chi.11711

KEGG: chx:100861232

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