Dog Interleukin 2,IL-2 ELISA Kit

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Code: CSB-E11258c

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 15.6 pg/mL-1000 pg/mL

Sensitivity: 3.9 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Alternative Names

IL2Interleukin-2 ELISA Kit; IL-2 ELISA Kit; T-cell growth factor ELISA Kit; TCGF ELISA Kit

Abbreviation

IL2

Uniprot NO.

Q29416

Species

Canis lupus familiaris (Dog) (Canis familiaris)

Sample Type

serum, plasma, tissue homogenates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	81-89
1:2	Average %	95
	Range %	92-98
1:4	Average %	86
	Range %	83-90
1:8	Average %	97
	Range %	94-100

Recovery

The recovery of dog IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	82-90
EDTA plasma (n=4)	105	101-109

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.919	2.900	2.910	2.812
500	2.424	2.468	2.446	2.348
250	1.670	1.620	1.645	1.547
125	0.981	0.967	0.974	0.876
62.5	0.474	0.481	0.478	0.380
31.2	0.289	0.276	0.283	0.185
15.6	0.161	0.163	0.162	0.064
0	0.099	0.097	0.098

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

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Dog Interleukin 2,IL-2 ELISA Kit

Description

This Dog IL2 ELISA Kit was designed for the quantitative measurement of Dog IL2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

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Citations

Target Background

Function(From Uniprot)

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.

Gene References into Functions

immunoreactivities of inflammatory cytokines, such as interleukin (IL)-2, its receptor (IL-2R), IL-4 and its receptor (IL-4R) in the cervical and lumbar spinal cord of young adult and aged beagle dogs, were compared. PMID:23605681
In dogs with leishmaniasis, there was high expression of genes encoding IL-2 in lymph nodes. PMID:21511273

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Subcellular Location

Secreted.

Protein Families

IL-2 family

Database Links

UNIGENE: Cfa.7

KEGG: cfa:403989

STRING: 9615.ENSCAFP00000005941

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