Dog Erythropoietin,EPO ELISA Kit

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Code: CSB-E11303c

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 1.56 mIU/mL-100 mIU/mL

Sensitivity: 0.39 mIU/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Target Background

Product Details

Alternative Names

EPOErythropoietin ELISA kit

Abbreviation

EPO

Uniprot NO.

P33707

Species

Canis lupus familiaris (Dog) (Canis familiaris)

Sample Type

serum, plasma, tissue homogenates

Detection Range

1.56 mIU/mL-100 mIU/mL

Sensitivity

0.39 mIU/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of dog EPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	91-105
1:2	Average %	89
	Range %	80-97
1:4	Average %	93
	Range %	86-100
1:8	Average %	95
	Range %	88-100

Recovery

The recovery of dog EPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	83-102
EDTA plasma (n=4)	95	85-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average	Corrected
100	1.902	1.893	1.898	1.725
50	1.505	1.518	1.512	1.339
25	1.087	1.146	1.117	0.944
12.5	0.804	0.789	0.797	0.624
6.25	0.499	0.512	0.506	0.333
3.12	0.342	0.351	0.347	0.174
1.56	0.255	0.261	0.258	0.085
0	0.170	0.176	0.173

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This Dog EPO ELISA Kit was designed for the quantitative measurement of Dog EPO protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 mIU/mL-100 mIU/mL and the sensitivity is 0.39 mIU/mL.

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Target Background

Function(From Uniprot)

Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.

Gene References into Functions

Developmental and compensatory lung growth involve paracrine erythropoietin signaling with parallel upregulation but differential processing of the erythropoietin receptor. PMID:15286000
apical secretion of EPO involves a cholesterol-dependent mechanism probably not involving lipid rafts PMID:15916748

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Subcellular Location

Secreted.

Protein Families

EPO/TPO family

Tissue Specificity

Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

Database Links

UNIGENE: Cfa.3871

KEGG: cfa:404002

STRING: 9615.ENSCAFP00000038507

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