Chicken Insulin-like growth factor 1, IGF-1 ELISA Kit

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Chicken Insulin-like growth factor 1, IGF-1 ELISA Kit

Name: Chicken Insulin-like growth factor 1, IGF-1 ELISA Kit
Brand: CUSABIO
SKU: CSB-E09867Ch

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Code: CSB-E09867Ch

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 125 pg/mL-2000 pg/mL

Sensitivity: 125 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Target Name

insulin-like growth factor 1 (somatomedin C)

Alternative Names

IGF1 ELISA Kit; Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit

Abbreviation

IGF1

Uniprot NO.

P18254

Species

Gallus gallus (Chicken)

Sample Type

serum, plasma, tissue homogenates

Detection Range

125 pg/mL-2000 pg/mL

Sensitivity

125 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Competitive

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of chicken IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-96
1:2	Average %	97
	Range %	92-105
1:4	Average %	87
	Range %	85-90
1:8	Average %	96
	Range %	93-99

Recovery

The recovery of chicken IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-94
EDTA plasma (n=4)	95	90-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
2000	0.183	0.194	0.189
1000	0.311	0.333	0.322
500	0.526	0.500	0.513
250	1.024	1.028	1.026
125	2.107	2.120	2.114

Troubleshooting and FAQs

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Description

This Chicken IGF1 ELISA Kit was designed for the quantitative measurement of Chicken IGF1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 125 pg/mL-2000 pg/mL and the sensitivity is 125 pg/mL.

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Dietary Supplementation of Purified Amino Acid Derived from Animal Blood on Immune Response and Growth Performance of Broiler Chicken T. G. Wandita.et al,/,2018
Chitosan oligosaccharide as an effective feed additive to maintain growth performance, meat quality, muscle glycolytic metabolism, and oxidative status in yellow-feather broilers under heat stress Q Chang,/,2020
Effects of dietary L-arginine on the production performance and gene expression of reproductive hormones in laying hens fed low crude protein diets VA Uyanga,/,2023
Dietary Clostridium butyricum and 25-Hydroxyvitamin D3 modulate bone metabolism of broilers through the gut–brain axis G Cao,/,2024
Dynamic changes in insulin-like growth factor binding protein expression occur between embryonic and early post-hatch development in broiler chickens LA Vaccaro, K Herring, A Wilson, E England, AL Smith,/,2024

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling.

Gene References into Functions

It is a protein synthesis-related factor. PMID:28594135
The coding region of the IGF1 gene was polymorphic, expressed differentially during the pre-hatch and post-hatch periods, and haplogroups showed significant association with growth traits in chicken. PMID:26059224
The IGF-I gene is significantly associated with some growth traits (body weight at hatching, and at 4, 8, 12, and 14 weeks of age) in chickens. PMID:23546986
IGF1 single-nucleotide polymorphism c.47673G > A was associated with body weight and haematocrit percentage, and also with feed intake and percentages of abdominal fat and gizzard genotype x sex interactions. PMID:23275255
Insulin-like growth factor 1 stimulates Non-astrocytic Inner Retinal Glial cells to proliferate, migrate distally into the retina, and become reactive. PMID:22973454
The objective of this study was to examine associations of an IGF1 promoter polymorphism with growth, body composition, skeleton integrity, and metabolic traits in chickens. PMID:15742956
Genetic/nutritional models show a positive relation between muscle IGF-1 mRNA levels, which determine paracrine IGF-1 levels, and post hatch muscle growth. (Review) PMID:16077194
These results suggest that the mechanism of action whereby IGFBP2 excess impairs long bone development is to inhibit IGF-I and IGF-II mediated proliferation and matrix synthesis. PMID:16183342
Polymorphisms within the IGF1 gene are associated with 5-week body weight. PMID:16734694
Blood levels ofIGF-I may be more important than IGF-II after chicken hatching PMID:17369538
Data show that transcription level of IGF-1 decreased dramatically in the sex-linked dwarf. PMID:17681929
Association analysis showed that haplotypes based on three cIGF1 polymorphisms (c.-366A>C, c.528G>A and c.*1024C>T) were associated with body weight traits, suggesting that cIGF1 or a tightly linked gene had effects on body weight in the chicken. PMID:18717967
IGF-1 genes and proteins are expressed at high or moderately high levels in healing tendon PMID:19084187