Chicken Insulin-like growth factor 1, IGF-1 ELISA Kit

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Chicken Insulin-like growth factor 1, IGF-1 ELISA Kit

Name: Chicken Insulin-like growth factor 1, IGF-1 ELISA Kit
Brand: CUSABIO
SKU: CSB-E09867Ch

CSB-E09867Ch

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Size:

96T,5×96T,10×96T

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Sample Types: serum, plasma, tissue homogenates

Detect Range: 125 pg/mL-2000 pg/mL

Sensitivity: 125 pg/mL

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Orders can only be placed from within the U.S.

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Alternative Names

IGF1 ELISA Kit; Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit

Abbreviation

IGF1

Uniprot NO.

P18254

Species

Gallus gallus (Chicken)

Sample Type

serum, plasma, tissue homogenates

Detection Range

125 pg/mL-2000 pg/mL

Sensitivity

125 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Competitive

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of chicken IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-96
1:2	Average %	97
	Range %	92-105
1:4	Average %	87
	Range %	85-90
1:8	Average %	96
	Range %	93-99

Recovery

The recovery of chicken IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-94
EDTA plasma (n=4)	95	90-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
2000	0.183	0.194	0.189
1000	0.311	0.333	0.322
500	0.526	0.500	0.513
250	1.024	1.028	1.026
125	2.107	2.120	2.114

Troubleshooting and FAQs

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Datasheet & COA

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Description

This Chicken IGF1 ELISA Kit was designed for the quantitative measurement of Chicken IGF1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 125 pg/mL-2000 pg/mL and the sensitivity is 125 pg/mL.

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Dietary Supplementation of Purified Amino Acid Derived from Animal Blood on Immune Response and Growth Performance of Broiler Chicken T. G. Wandita.et al,/,2018
Chitosan oligosaccharide as an effective feed additive to maintain growth performance, meat quality, muscle glycolytic metabolism, and oxidative status in yellow-feather broilers under heat stress Q Chang,/,2020
Effects of dietary L-arginine on the production performance and gene expression of reproductive hormones in laying hens fed low crude protein diets VA Uyanga,/,2023
Dietary Clostridium butyricum and 25-Hydroxyvitamin D3 modulate bone metabolism of broilers through the gut–brain axis G Cao,/,2024
Dynamic changes in insulin-like growth factor binding protein expression occur between embryonic and early post-hatch development in broiler chickens LA Vaccaro, K Herring, A Wilson, E England, AL Smith,/,2024

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Function

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling.

Gene References into Functions

It is a protein synthesis-related factor. PMID:28594135
The coding region of the IGF1 gene was polymorphic, expressed differentially during the pre-hatch and post-hatch periods, and haplogroups showed significant association with growth traits in chicken. PMID:26059224
The IGF-I gene is significantly associated with some growth traits (body weight at hatching, and at 4, 8, 12, and 14 weeks of age) in chickens. PMID:23546986
IGF1 single-nucleotide polymorphism c.47673G > A was associated with body weight and haematocrit percentage, and also with feed intake and percentages of abdominal fat and gizzard genotype x sex interactions. PMID:23275255
Insulin-like growth factor 1 stimulates Non-astrocytic Inner Retinal Glial cells to proliferate, migrate distally into the retina, and become reactive. PMID:22973454
The objective of this study was to examine associations of an IGF1 promoter polymorphism with growth, body composition, skeleton integrity, and metabolic traits in chickens. PMID:15742956
Genetic/nutritional models show a positive relation between muscle IGF-1 mRNA levels, which determine paracrine IGF-1 levels, and post hatch muscle growth. (Review) PMID:16077194
These results suggest that the mechanism of action whereby IGFBP2 excess impairs long bone development is to inhibit IGF-I and IGF-II mediated proliferation and matrix synthesis. PMID:16183342
Polymorphisms within the IGF1 gene are associated with 5-week body weight. PMID:16734694
Blood levels ofIGF-I may be more important than IGF-II after chicken hatching PMID:17369538
Data show that transcription level of IGF-1 decreased dramatically in the sex-linked dwarf. PMID:17681929
Association analysis showed that haplotypes based on three cIGF1 polymorphisms (c.-366A>C, c.528G>A and c.*1024C>T) were associated with body weight traits, suggesting that cIGF1 or a tightly linked gene had effects on body weight in the chicken. PMID:18717967
IGF-1 genes and proteins are expressed at high or moderately high levels in healing tendon PMID:19084187