Bovine anti-Mullerian hormone (AMH) ELISA kit

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Name: Bovine anti-Mullerian hormone (AMH) ELISA kit
Brand: CUSABIO
SKU: CSB-E13406B

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Code: CSB-E13406B

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 0.5 ng/mL-200 ng/mL

Sensitivity: 0.5 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Alternative Names

AMH ELISA Kit; Muellerian-inhibiting factor ELISA Kit; Anti-Muellerian hormone ELISA Kit; AMH ELISA Kit; Muellerian-inhibiting substance ELISA Kit; MIS ELISA Kit

Abbreviation

AMH

Uniprot NO.

P03972

Species

Bos taurus (Bovine)

Sample Type

serum, plasma, tissue homogenates

Detection Range

0.5 ng/mL-200 ng/mL

Sensitivity

0.5 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Competitive

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine AMH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-101
1:2	Average %	98
	Range %	91-105
1:4	Average %	92
	Range %	88-97
1:8	Average %	97
	Range %	93-105

Recovery

The recovery of bovine AMH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-99
EDTA plasma (n=4)	97	90-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
200	0.134	0.139	0.137
37.5	0.211	0.203	0.207
6.25	0.495	0.485	0.490
1.75	0.968	0.987	0.978
0.5	1.146	1.154	1.150
0	2.226	2.250	2.238

Troubleshooting and FAQs

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ELISA Data Analysis

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Description

This Bovine AMH ELISA Kit was designed for the quantitative measurement of Bovine AMH protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.5 ng/mL-200 ng/mL and the sensitivity is 0.5 ng/mL .

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

This glycoprotein, produced by the Sertoli cells of the testis, causes regression of the Muellerian duct. It is also able to inhibit the growth of tumors derived from tissues of Muellerian duct origin.

Gene References into Functions

Anti-Mullerian hormone inhibits activation and growth of bovine ovarian follicles in vitro and is localized to growing follicles. PMID:28333275
This study showed that antral follicle counts (AFC) and AMH levels were repeatable when determined at an unknown stage of follicular growth and expected day of follicular wave emergence, but repeatability was greater for AMH than AFC. PMID:28237349
The effects of castration and other surgical intervention on the blood levels of anti-Muellerian hormone, inhibin A, gonadotropins, and gonadotropin receptors in bull calves are reported. PMID:27693012
Results from these studies indicate that AMH signaling plays a role in both regulating granulosa cell proliferation and preventing granulosa cells from 5- to 8-mm follicles from undergoing premature differentiation before follicle selection. PMID:27268296
These findings indicate the followings: AMH mRNA levels decrease in both dominant and secondary follicles during follicular deviation; granulosa cells from heathy follicles express more AMH mRNA compared to subordinate follicles undergoing atresia and FSH stimulates AMH and AMHR2 mRNA expression in granulosa cells of co-dominant follicles. PMID:26815645
Measurement of AMH concentration in the plasma of cows can help to predict their capacity for embryo production in response to gonadotrophin treatment. PMID:22935154
In first study to investigate the blood profile and immunohuistochemistry of anti-Mullerian hormone in bovine granulosa-theca cell tumors, the findings indicated that anti-Mullerian hormone is a novel biomarker for granulosa-theca cell tumors in cattle. PMID:22052009
Regulation of anti-Mullerian hormone production in the cow. PMID:21076084
Intrafollicular AMH was not a marker of cystic development in the cow, but low AMH concentrations in cysts were associated with luteinization. PMID:18448844
AMH expression is modulated by androgens in bovine granulosa cells from small follicles. PMID:19714502