Bovine Interleukin 10,IL-10 ELISA Kit

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Target Background

Name: Bovine Interleukin 10,IL-10 ELISA Kit
Brand: CUSABIO
SKU: CSB-E12917B

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Code: CSB-E12917B

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Citation （18）

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 5 pg/mL-1000 pg/mL

Sensitivity: 2.5 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Target Background

Target Name

interleukin 10

Alternative Names

IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit

Abbreviation

IL10

Uniprot NO.

P43480

Species

Bos taurus (Bovine)

Sample Type

serum, plasma, tissue homogenates

Detection Range

5 pg/mL-1000 pg/mL

Sensitivity

2.5 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Competitive

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-98
1:2	Average %	95
	Range %	91-98
1:4	Average %	96
	Range %	87-102
1:8	Average %	97
	Range %	91-104

Recovery

The recovery of bovine IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	95-100
EDTA plasma (n=4)	96	92-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
1000	0.098	0.095	0.097
250	0.301	0.285	0.293
80	0.487	0.458	0.473
20	0.972	0.975	0.974
5	1.364	1.316	1.340
0	2.128	2.211	2.170

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Bovine Interleukin 10,IL-10 ELISA Kit

Description

Interleukin 10 (IL-10) is a potent anti-inflammatory cytokine that plays a key role in immune regulation and homeostasis. Regulatory T cells, macrophages, and B cells produce IL-10, which suppresses inflammatory responses by inhibiting pro-inflammatory cytokine production and limiting antigen presentation. This immunosuppressive activity makes IL-10 essential for preventing excessive immune activation. It also maintains tissue integrity during inflammatory processes. Research into IL-10 levels provides valuable insights into immune dysfunction, autoimmune diseases, and inflammatory conditions across various species.

The Bovine Interleukin 10, IL-10 ELISA Kit (CSB-E12917B) is designed for quantitative measurement of IL-10 in Bos taurus samples. This competitive assay works with serum, plasma, and tissue homogenates. The detection range spans 5 pg/mL to 1000 pg/mL with sensitivity of 2.5 pg/mL. The assay requires 50-100 μL sample volume and can be completed within 1-5 hours. Detection occurs at 450 nm wavelength, providing researchers with a reliable tool for bovine IL-10 quantification in immunological studies.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This bovine interleukin 10 ELISA kit has been used in research investigating immune responses and inflammatory processes in cattle. The kit allows quantitative measurement of IL-10 levels in various biological samples to determine anti-inflammatory cytokine profiles and immune system function.

• Immune response studies: Measurement of immunological parameters alongside other immune markers including immunoglobulins and pro-inflammatory cytokines in serum samples
• Antioxidant and inflammation research: Analysis of anti-inflammatory cytokine levels in conjunction with oxidative stress markers to understand the relationship between antioxidant capacity and immune function
• Pro-inflammatory response analysis: Measurement of anti-inflammatory cytokines in plasma samples as part of comprehensive cytokine profiling studies
• Tissue-based cytokine analysis: Quantification of IL-10 levels in lymph node tissue samples for localized immune response studies

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.

Gene References into Functions

Studied expression of Interleukin 10 (IL-10) in blood and milk samples of 25 healthy and 25 mastitic cows. Found IL-10 expression to be significantly higher in the blood and milk samples of mastitic cows compared to the healthy ones. PMID:28267404
This study seems to indicate that PGE2 in cattle does not produce an anti-inflammatory effect by increasing the synthesis of IL-10. PMID:25286662
IL-10 mRNA expression increased on day 8 in the mononuclear leukocytes of pregnant cows. In a cell culture experiment, interferon-tau stimulated stimulated IL-10 mRNA expression in mononuclear leukocytes. PMID:22052007
Bovine IL-10 potently inhibits the activation of human myeloid cells in response to toll like receptor activation. PMID:21464967
Levels in milk are altered in the presence of bacteria. PMID:15372997
Monocytes obtained from cows with subclinical infection with MAP had upregulated expression of IL-10 and SOCS-3, which may have attenuated the capacity of mononuclear phagocytes to initiate inflammatory and adaptive immune responses. PMID:16111147