S100a9 Antibody

Code: CSB-PA020642ZA01RA

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Size:

0.1ml

0.1ml1ml

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Species Reactivity: Rattus norvegicus

Raised in: Rabbit

Application: ELISA, WB (ensure identification of antigen)

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Target Background

Product Details

Uniprot NO.

P50116

Target Names

S100a9

Alternative Names

S100a9 antibody; Mrp14Protein S100-A9 antibody; Calgranulin-B antibody; Migration inhibitory factor-related protein 14 antibody; MRP-14 antibody; p14 antibody; Myeloid-related protein 14 antibody; S100 calcium-binding protein A9 antibody

Raised in

Rabbit

Species Reactivity

Rattus norvegicus

Immunogen

Recombinant Rattus norvegicus S100a9 protein

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Tested Applications

ELISA, WB (ensure identification of antigen)

Lead Time

Made-to-order (12-14 weeks)
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

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Target Background

Function(From Uniprot)

S100A9 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis, adhesion, can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinflammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. The iNOS-S100A8/A9 transnitrosylase complex is proposed to direct selective inflammatory stimulus-dependent S-nitrosylation of multiple targets such as GAPDH, NXA5, EZR, MSN and VIM by recognizing a [IL]-x-C-x-x-[DE] motif.

Gene References into Functions

these results suggest that calprotectin, along with other inflammatory factors, promotes the inflammation seen in mild food allergy PMID:28454097
Recombinant rat r-S100A9 bound to r-CD68. The expression levels of S100A9 were significantly suppressed after the macrophages had been treated with an anti-CD68 antibody. Small interfering CD68 also significantly suppressed activation of macrophages through an autocrine pathway by r-S100A9. PMID:27312849
In dextran sulfate sodium-induced colitis fecal levels of S100A9 detected by ELISA were significantly elevated before microscopic damage was detected. PMID:27693389
Decreased S100A9 expression promoted airway smooth muscle cell proliferation by stimulating reactive oxygen species generation and inhibiting p38 MAPK. PMID:28050155
Expression of S100A9 mRNA and the corresponding protein in cultured NRVCs was similar between control cells and NE-stimulated cells, but was markedly upregulated at 12 hours after the withdrawal of NE PMID:25820336
in ulcerative colitis rats the immune functions of macrophages are regulated in a complex manner by r-S100A8 and/or r-S100A9 through undefined autocrine pathways on the cells PMID:25485702
S100a9 is a memory-related gene, which is affected by the presence of occlusal support in rats. PMID:24370184
Data indicate that serum calprotectin levels correlated with serum levels of TNF-alpha and IL6 in TNBS colitis. PMID:23685388
results suggest that the increased serum S100A8/A9 concentrations following acute myocardial infarction were not of cardiac myocyte origin PMID:21887593
To understand the possible role of S100A9 in asthma, the effects of the S100A9 were investigated in a rat model of asthma and in isolated tracheal spirals. PMID:20599758
Phosphorylation inhibits tubulin polymerization in microtubules. PMID:15331440
An interaction between a subpopulation of neutrophils and the central nervous system during peripheral tissue inflammation is implied by the apparent release and possible diffusion of S100A8 and S100A9 through the endothelial blood-brain barrier. PMID:18063312