ALG11 Antibody

Code: CSB-PA894498XA01DOA

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Size:

0.1ml

0.1ml1ml

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Species Reactivity: Arabidopsis thaliana

Raised in: Rabbit

Application: ELISA, WB (ensure identification of antigen)

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Target Background

Product Details

Uniprot NO.

Q9XEE9

Target Names

ALG11

Alternative Names

ALG11; LEW3; At2g40190; T07M07.7; GDP-Man:Man(3GlcNAc(2-PP-Dol alpha-1,2-mannosyltransferase; Alpha-1,2-mannosyltransferase ALG11; Asparagine-linked glycosylation protein 11; Protein LEAF WILTING 3

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana ALG11 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Tested Applications

ELISA, WB (ensure identification of antigen)

Lead Time

Made-to-order (14-16 weeks)
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

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Target Background

Function(From Uniprot)

Required for N-linked oligosaccharide assembly. Has a role in the last step of the synthesis of the Man(5)GlcNAc(2)-PP-dolichol core oligosaccharide on the cytoplasmic face of the endoplasmic reticulum.

Gene References into Functions

Positional cloning determined that lrg1 affects an alpha-1,2-mannosyltransferase gene, LEW3, involved in protein N-glycosylation. The lrg1 mutation causes a synonymous substitution that alters the correct splicing of the fourth intron in LEW3, causing a mix of wild-type and truncated protein. LRG1 RNA missplicing in roots and short root phenotypes in lrg1 are light-intensity dependent. PMID:29106622
The lew3 mutation reduces protein glycosylation, impairs cellulose synthesis, and results in xylem collapse. lew3 mutants are more sensitive to salt stress and transpire less water than the wild-type. PMID:19732381

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Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Protein Families

Glycosyltransferase 4 family

Tissue Specificity

Expressed in shoots, leaves, sepals, filaments, siliques, stems, roots and guard cells.

Database Links

UNIGENE: At.21597

KEGG: ath:AT2G40190

STRING: 3702.AT2G40190.1

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