Abbreviation
Recombinant Mouse Cxcl5 protein (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration of 10-100 ng/ml.
Alternative Names
Cxcl5; Scyb5C-X-C motif chemokine 5; Cytokine LIX; Small-inducible cytokine B5) [Cleaved into: GCP-2(1-78); GCP-2(9-78)]
Species
Mus musculus (Mouse)
Expression Region
41-132aa
Target Protein Sequence
APSSVIAATE LRCVCLTVTP KINPKLIANL EVIPAGPQCP TVEVIAKLKN QKEVCLDPEA PVIKKIIQKI LGSDKKKAKR NALAVERTAS VQ
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PBS, pH 7.4, 150 mM NaCl.
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CXCL5 (LIX) is a potent ELR⁺ CXC chemokine that drives neutrophil recruitment through CXCR2 signaling, making it a critical tool for dissecting innate immune responses in murine models. This tag-free recombinant mouse CXCL5, encompassing the full-length mature sequence (residues 41–132), induces robust chemotaxis of human peripheral blood neutrophils at 10–100 ng/ml, confirming its suitability for neutrophil migration assays, CXCR2-dependent MAPK and NF-κB signaling studies, and in vivo inflammation models. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that can confound neutrophil activation readouts, while purity exceeding 97% by SDS-PAGE supports use as a reliable reference standard in cytokine detection assays or as a coating antigen for anti-CXCL5 antibody validation by ELISA. Generated in *E. coli*, this protein provides a suitable basis for functional studies where sequence-authentic, contaminant-controlled chemokine is essential.
