Abbreviation
Recombinant Mouse Cxcl5 protein, partial (Active)
Purity
>96% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemoattract bioassay using human neutrophils is in a concentration range of 10-100 ng/ml.
Alternative Names
Cxcl5; Scyb5C-X-C motif chemokine 5; Cytokine LIX; Small-inducible cytokine B5) [Cleaved into: GCP-2(1-78); GCP-2(9-78)]
Species
Mus musculus (Mouse)
Expression Region
49-118aa
Target Protein Sequence
TELRCVCLTV TPKINPKLIA NLEVIPAGPQ CPTVEVIAKL KNQKEVCLDP EAPVIKKIIQ KILGSDKKKA
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CXCL5 (LIX) is a key ELR⁺ CXC chemokine that drives neutrophil recruitment during acute inflammatory responses, making precise functional characterization essential for murine inflammation research. This tag-free recombinant mouse CXCL5 (residues 49–118) corresponds to the mature processed form and demonstrates confirmed chemoattractant activity on human neutrophils at 10–100 ng/ml, providing a suitable basis for neutrophil chemotaxis and transwell migration assays, as well as downstream CXCR2-mediated MAPK and NF-κB signaling studies. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that can confound immune cell activation readouts, supporting use in sensitive in vivo mouse models of inflammation or infection and in cell-based functional assays where neutrophil responses must be attributed specifically to CXCL5 signaling. Purity exceeding 96% by SDS-PAGE further satisfies the criteria expected for antibody development, ELISA coating antigen preparation, and use as a reference standard in cytokine detection platforms.
