Abbreviation
Recombinant Mouse Cxcl14 protein (Active)
Purity
>95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/ml.
Alternative Names
Cxcl14; Bmac; Kec; Ks1; Mip2g; Scyb14C-X-C motif chemokine 14; B-cell and monocyte-activating chemokine; Chemokine BRAK; Kidney-expressed chemokine CXC; MIP-2G; Small-inducible cytokine B14
Species
Mus musculus (Mouse)
Expression Region
23-99aa
Target Protein Sequence
SKCKCSRKGP KIRYSDVKKL EMKPKYPHCE EKMVIVTTKS MSRYRGQEHC LHPKLQSTKR FIKWYNAWNE KRRVYEE
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CXCL14 is a homeostatic chemokine with potent monocyte-recruiting activity, yet its signaling mechanisms remain incompletely characterized, making a reliable recombinant form essential for mechanistic studies. This tag-free recombinant mouse CXCL14 encompasses the full-length mature sequence (residues 23–99) and demonstrates robust chemotactic activity on human monocytes at 1.0–10 ng/ml, providing a suitable basis for chemotaxis and transwell migration assays, monocyte activation studies, and downstream investigation of NF-κB and MAPK signaling pathways. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that can confound innate immune cell readouts, which supports use in sensitive functional assays and in vivo inflammation or tumor biology models in mice. Purity exceeding 95% by SDS-PAGE, combined with the low endotoxin profile, also satisfies the quality thresholds commonly required for ELISA standard curves, antibody validation, and immunogen applications.
