Abbreviation
Recombinant Mouse Cxcl11 protein (Active)
Purity
>98% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using murine CXCR3 transfected 293 cells is in a concentration of 10-100 ng/ml.
Alternative Names
Cxcl11; Scyb11C-X-C motif chemokine 11; Interferon-inducible T-cell alpha chemoattractant; I-TAC; Small-inducible cytokine B11
Species
Mus musculus (Mouse)
Expression Region
22-100aa
Target Protein Sequence
FLMFKQGRCL CIGPGMKAVK MAEIEKASVI YPSNGCDKVE VIVTMKAHKR QRCLDPRSKQ ARLIMQAIEK KNFLRRQNM
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CXCL11 (I-TAC) is a potent CXCR3 ligand that drives Th1-biased lymphocyte trafficking, making it a critical tool for dissecting interferon-regulated immune responses. This tag-free recombinant mouse CXCL11, spanning the full mature sequence (22–100 aa), demonstrates confirmed chemotactic activity at 10–100 ng/ml in a migration bioassay using murine CXCR3-transfected 293 cells, providing a reliable basis for Transwell chemotaxis assays, CXCR3 signaling pathway studies involving MAPK or JAK/STAT activation, and in vivo models of inflammation or tumor immune infiltration. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that can confound cytokine-dependent readouts in primary T-cell activation or macrophage polarization experiments. Purity exceeding 98% by SDS-PAGE, combined with the low endotoxin profile, also supports use as a reference standard in cytokine detection assays or as a coating antigen for antibody validation by ELISA.
