Abbreviation
Recombinant Mouse Ccl24 protein (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using murine lymphocytes is in a concentration of 10-100 ng/ml.
Alternative Names
Ccl24; Scya24; C-C motif chemokine 24; Eosinophil chemotactic protein 2; Eotaxin-2; Small-inducible cytokine A24
Species
Mus musculus (Mouse)
Expression Region
27-119aa
Target Protein Sequence
VTIPSSCCTS FISKKIPENR VVSYQLANGS ICPKAGVIFI TKKGHKICTD PKLLWVQRHI QKLDAKKNQP SKGAKAVRTK FAVQRRRGNS TEV
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CCL24 (eotaxin-2) is a potent CC chemokine that selectively recruits eosinophils and basophils via CCR3, making it a critical mediator in allergic inflammation and type 2 immune responses. This tag-free recombinant mouse CCL24, encompassing the full-length mature sequence (residues 27–119), demonstrates confirmed biological activity in murine lymphocyte chemotaxis bioassays at 10–100 ng/ml, providing a suitable basis for Transwell migration assays, CCR3 signaling studies involving MAPK and calcium flux pathways, and in vivo models of airway or skin inflammation. The endotoxin level of less than 1.0 EU/μg satisfies the stringent criteria needed to prevent LPS-driven artifacts in sensitive immune cell functional assays, while purity exceeding 97% by SDS-PAGE supports its use as a reliable reference standard in cytokine detection ELISAs or as a positive control for anti-CCL24 antibody validation. Produced in *E. coli*, this preparation aligns with standards expected in quantitative receptor-ligand binding and desensitization experiments.
