Abbreviation
Recombinant Mouse Ccl12 protein (Active)
Purity
>95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-50 ng/ml.
Alternative Names
C-C motif chemokine 12; Ccl12; CCL12_MOUSE; Macrophage/monocyte Chemotactic Protein; MCP-1-related chemokine; MCP-5; Monocyte chemoattractant protein 5; Monocyte chemotactic protein 5; OTTMUSP00000000792; Scya12; Small inducible cytokine A12; Small-inducible cytokine A12
Species
Mus musculus (Mouse)
Expression Region
23-104aa
Target Protein Sequence
GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CCL12 (MCP-5) is a potent monocyte chemoattractant unique to mice, making it an essential tool for studying monocyte recruitment in murine models of inflammation, fibrosis, and tumor biology. This tag-free recombinant protein encompasses the full-length mature sequence (residues 23–104) and demonstrates confirmed biological activity in a chemotaxis bioassay using human peripheral blood monocytes at 10–50 ng/ml, supporting its use in Transwell migration assays, CCR2 signaling pathway studies involving MAPK and NF-κB activation, and as a reference standard in cytokine detection platforms. Endotoxin levels below 1.0 EU/μg minimize the risk of LPS-driven artifacts in sensitive immune cell functional assays, while purity exceeding 95% by SDS-PAGE provides a suitable basis for antibody development workflows including immunization, ELISA coating, and Western blot positive controls. The E. coli-derived protein offers a non-glycosylated format that aligns with standards expected in quantitative binding and activity benchmarking experiments.
