Abbreviation
Recombinant Human CXCL12 protein, partial (Active)
Purity
>96% as determined by SDSPAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using PHA and rHuIL?2 activated human peripheral blood T?lymphocytes is in a concentration range of 30?100 ng/ml.
Alternative Names
12-O-tetradecanoylphorbol 13-acetate repressed protein 1; AI174028; C-X-C motif chemokine 12; Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1); Chemokine (C-X-C motif) ligand 12; Chemokine CXC motif ligand 12; cxcl12; hIRH; hSDF-1; Intercrine reduced in hepatomas; IRH; OTTHUMP00000019491 ; PBSF; Pre-B cell growth-stimulating factor; SCYB12; SDF 1; SDF-1; SDF-1-alpha(3-67); SDF-1a; SDF-1b; SDF1_HUMAN; SDF1A; SDF1B; Stromal cell-derived factor 1; Stromal cell-derived factor 1 delta ; Stromal cell-derived factor 1 gamma ; Stromal cell-derived factor 1a ; Stromal cell-derived factor-1 alpha ; Thymic lymphoma cell-stimulating factor; Tlsf; TLSF-a; TLSF-b; Tlsfa; Tlsfb; TPAR1
Species
Homo sapiens (Human)
Expression Region
21-119aa
Target Protein Sequence
GKPVSLSYRC PCRFFESHVA RANVKHLKIL NTPNCALQIV ARLKNNNRQV CIDPKLKWIQ EYLEKALNKG RREEKVGKKE KIGKKKRQKK RKAAQKRKN
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Partial of Isoform Gamma
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
0.2 μm filtered PBS, pH 7.4 ,lyophilized
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CXCL12/SDF-1 is a pivotal chemokine that signals through CXCR4 and CXCR7 to regulate lymphocyte trafficking, hematopoietic stem cell homing, and tumor metastasis, making validated recombinant forms essential for mechanistic immunology research. This tag-free partial construct (residues 21–119 of Isoform Gamma), expressed in *E. coli* to preserve the mature sequence without eukaryotic post-translational modifications that could confound receptor-binding studies, demonstrates confirmed chemotactic activity at 30–100 ng/mL in a bioassay using PHA/IL-2-activated human peripheral blood T lymphocytes. That functional potency supports use in transwell migration assays, CXCR4-mediated MAPK and NF-κB signaling studies, and as a reference standard in chemokine detection platforms. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts in sensitive immune cell experiments, while purity exceeding 96% by SDS-PAGE aligns with standards expected in antibody validation workflows such as ELISA coating and Western blot positive controls.
