Abbreviation
Recombinant Human XCL1 protein, partial (Active)
Purity
>98% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration of 10-100 ng/ml.
Alternative Names
ATAC; C motif chemokine 1; Chemokine (C motif) ligand 1; Chemokine C Motif Ligand 1; Cytokine SCM-1; LPTN; LTN; Lymphotactin; Lymphotaxin; SCM 1 alpha; SCM 1; SCM 1a; SCM-1-alpha; SCM1; SCM1A; SCYC1; Single cysteine motif 1a; Small inducible cytokine C1; Small Inducible Cytokine Subfamily C Member 1; Small inducible cytokine subfamily C, member 1 (lymphotactin); Small-inducible cytokine C1; X-C motif chemokine ligand 1; XC chemokine ligand 1; Xcl1; XCL1_HUMAN
Species
Homo sapiens (Human)
Expression Region
23-114aa
Target Protein Sequence
GSEVSDKRTC VSLTTQRLPV SRIKTYTITE GSLRAVIFIT KRGLKVCADP QATWVRDVVR SMDRKSNTRN NMIQTKPTGT QQSTNTAVTL TG
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
Lymphotactin (XCL1) is the sole C-class chemokine in humans, signaling exclusively through XCR1 to recruit T cells and dendritic cell subsets—making it a critical tool for dissecting XCR1-mediated immune crosstalk. This tag-free recombinant protein spans residues 23–114, corresponding to the mature secreted sequence, and drives chemotaxis of primary human T-lymphocytes at 10–100 ng/ml, providing a suitable basis for Transwell migration assays, XCR1 receptor internalization studies, and downstream MAPK or calcium flux signaling experiments. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that commonly confound immune cell functional readouts, supporting use in sensitive in vivo inflammation models and primary cell activation assays where cytokine-specific effects must be cleanly resolved. Purity exceeding 98% by SDS-PAGE further qualifies this protein as a reliable reference standard in XCL1 detection ELISAs and as a positive control for antibody validation in Western blot.
