Abbreviation
Recombinant Human CCL7 protein (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml.
Alternative Names
C-C motif chemokine 7; Ccl7; CCL7_HUMAN; Chemokine CC motif ligand 7; FIC; MARC; MCP-3; Monocyte chemoattractant protein 3; Monocyte chemotactic protein 3; NC28; RP23-350G1.4; SCYA6; SCYA7; Small-inducible cytokine A7
Species
Homo sapiens (Human)
Expression Region
24-99aa
Target Protein Sequence
QPVGINTSTT CCYRFINKKI PKQRLESYRR TTSSHCPREA VIFKTKLDKE ICADPTQKWV QDFMKHLDKK TQTPKL
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered 20 mM PB, pH 7.4, 150 mM NaCl
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CCL7 (MCP-3) is a potent CC chemokine that recruits monocytes, dendritic cells, and lymphocytes through CCR1, CCR2, and CCR3 signaling, making it a critical mediator in inflammatory cell trafficking. This tag-free recombinant human CCL7, encompassing the full mature sequence (residues 24–99), demonstrates confirmed chemotactic activity on primary human monocytes at 10–100 ng/mL, providing a suitable basis for Transwell migration assays, monocyte activation studies, and downstream investigation of MAPK and NF-κB signaling pathways. The endotoxin level of less than 1.0 EU/μg minimizes LPS-driven artifacts that can confound immune cell functional readouts, supporting use in sensitive in vivo inflammation models and primary cell-based experiments where cytokine-specific effects must be distinguished from endotoxin contamination. Purity exceeding 97% by SDS-PAGE, combined with this stringent endotoxin profile, also satisfies the criteria typical for ELISA reference standards, antibody validation controls, and immunogen preparation.
