Abbreviation
Recombinant Human CCL13 protein, partial (Active)
Purity
>96% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 10-100 ng/ml.
Alternative Names
C-C motif chemokine 13; CCL13; CCL13_HUMAN; Chemokine (C C motif) ligand 13; CK beta 10; CK-beta-10; CKb10; MCP-4; MGC17134; Monocyte chemoattractant protein 4; Monocyte chemotactic protein 4; NCC 1; NCC-1; NCC1; New CC chemokine 1; SCYA13; SCYL1; short chain; Small inducible cytokine A13 precursor; Small inducible cytokine subfamily A (Cys Cys) member 13; Small-inducible cytokine A13
Species
Homo sapiens (Human)
Expression Region
24-98aa
Target Protein Sequence
QPDALNVPST CCFTFSSKKI SLQRLKSYVI TTSRCPQKAV IFRTKLGKEI CADPKEKWVQ NYMKHLGRKA HTLKT
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CCL13 (MCP-4) plays a non-redundant role in monocyte and eosinophil recruitment through CCR2 and CCR3 signaling, making it a critical reagent for dissecting chemokine-driven immune cell trafficking. This tag-free recombinant protein, spanning the mature sequence (residues 24–98) expressed in *E. coli*, demonstrates confirmed chemotactic activity on primary human monocytes at 10–100 ng/ml, providing a suitable basis for Transwell migration assays, CCR2/CCR3 receptor activation studies, and downstream MAPK or NF-κB signaling pathway analysis. The endotoxin level of <1.0 EU/μg satisfies the stringent criteria needed to prevent LPS-driven artifacts in sensitive monocyte and dendritic cell functional assays, while also supporting use in *in vivo* inflammation models where endotoxin contamination could confound results. Purity exceeding 96% by SDS-PAGE further supports its application as a reliable reference standard in chemokine detection assays or as a coating antigen for antibody development and ELISA validation.
