Capture: CSB-EAP731137C Detection: CSB-EAP731137D(HRP) Reagents are sufficient for at least 5 x 96 well plates using recommended protocol.
Tested Applications
S-ELISA
Notes
We recommend using the capture antibody at a concentration of 2ug/ml and the detection antibody at a concentration of 0.2ug/ml.Optimal dilutions should be determined experimentally by the researcher.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
CSB-EAP731137 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). An antibody specific for African swine fever virus Envelope protein p54 (Ba71V-126) has been pre-coated onto the microwells. The African swine fever virus Envelope protein p54 (Ba71V-126) protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody HRP conjugated specific for African swine fever virus Envelope protein p54 (Ba71V-126) is added to detect the captured African swine fever virus Envelope protein p54 (Ba71V-126) protein. Followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.
Envelope protein involved, through its interaction with host dynein, in the intracellular microtubule-dependent transport of viral capsid toward viral factories. Seems to induce caspase-3 activation and apoptosis. Plays a role in virion morphogenesis by recruiting and transforming the host ER membranes into the precursors of the viral envelope.