TBK1

TBK1, TANK-binding kinase 1. also known as T2K, NAK, etc. TBK1 is a serine/threonine protein kinase belonging to the non-classical IKK kinase family.
The mechanism of action of TBK1 involves its kinase activity, in particular phosphorylation of the Ser172 site in the kinase's structural domain, which is a key regulatory site for the kinase activity of TBK1. TBK1 plays a key role in the intrinsic immune system, activating NF-κB transcription factors and interferon regulatory factors (IRFs), which are essential for resistance to invasion by pathogenic microorganisms and the production of inflammatory cytokines.
Abnormal TBK1 activity is associated with a wide range of diseases including inflammatory diseases, autoimmune disorders, cancers, and neurodegenerative diseases, and is an emerging therapeutic target for drug discovery.

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

TBK1 Recombinant Monoclonal Antibody(CSB-RA932866A0HU)

Validated Data

IHC image of CSB-RA932866A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA932866A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Overlay Peak curve showing A549 cells stained with CSB-RA932866A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

The following TBK1 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

TBK1 Antibodies

TBK1 Proteins

TBK1 Antibodies

TBK1 Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA009019	TBK1 Antibody	Human,Mouse	IHC, ELISA
CSB-PA023232LA01HU	TBK1 Antibody	Human, Mouse	ELISA, WB, IHC, IF
CSB-PA023232LC01HU	TBK1 Antibody, FITC conjugated	Human
CSB-PA023232LD01HU	TBK1 Antibody, Biotin conjugated	Human	ELISA
CSB-PA890690NA01HU	TBK1 Antibody	Human	ELISA, WB, IF
CSB-PA890690NB01HU	TBK1 Antibody, HRP conjugated	Human	ELISA
CSB-PA890690NC01HU	TBK1 Antibody, FITC conjugated	Human
CSB-PA890690ND01HU	TBK1 Antibody, Biotin conjugated	Human	ELISA
CSB-PA890690NA172nphHU	TBK1 (Ab-172) Antibody	Human	ELISA, IHC
CSB-RA932866A0HU	TBK1 Recombinant Monoclonal Antibody	Human	ELISA, IHC, FC

TBK1 Proteins

TBK1 Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP890690HU1 CSB-EP890690HU1 CSB-BP890690HU1 CSB-MP890690HU1 CSB-EP890690HU1-B	Recombinant Human Serine/threonine-protein kinase TBK1 (TBK1), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-EP890690HU1	Recombinant Human Serine/threonine-protein kinase TBK1 (TBK1), partial	E.coli

TBK1 Proteins for Mus musculus (Mouse)

Code	Product Name	Source
CSB-YP894827MO CSB-EP894827MO CSB-BP894827MO CSB-MP894827MO CSB-EP894827MO-B	Recombinant Mouse Serine/threonine-protein kinase TBK1 (Tbk1)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

TBK1 Proteins for Xenopus laevis (African clawed frog)

Code	Product Name	Source
CSB-YP732220XBE CSB-EP732220XBE CSB-BP732220XBE CSB-MP732220XBE CSB-EP732220XBE-B	Recombinant Xenopus laevis Serine/threonine-protein kinase TBK1 (tbk1), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

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TBK1

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