LGR5

CSB-MP012906HU(A4) is detected by Mouse anti-10*His monoclonal antibody.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human LGR5 at 10 μg/ml can bind Anti-LGR5 recombinant antibody (CSB-RA012906MA1HU). The EC₅₀ is 1.690-1.867 ng/mL.The VLPs (CSB-MP3838) is negative control.

LGR5 Recombinant Monoclonal Antibody

IHC image of CSB-PA012906LA01HU diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with CSB-PA012906LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).