IGF1R, the insulin-like growth factor 1 receptor, is also known as CD221. This transmembrane receptor tyrosine kinase binds insulin-like growth factor 1 (IGF-1) with high affinity, leading to autophosphorylation and activation of downstream signaling cascades. It mainly functions through the PI3K/Akt and Ras/MAPK pathways, which are crucial for cell growth, proliferation, and survival.
Aberrant activation of IGF1R is associated with various human diseases, including several types of cancer such as breast, prostate, and lung cancer. Given its role in tumorigenesis, IGF1R has become an attractive target for drug development. Multiple small - molecule inhibitors and monoclonal antibodies are under pre - clinical and clinical investigations, aiming to block its activity and combat related diseases.
Popular
Product:
IGF1R Recombinant Monoclonal Antibody(CSB-RA011087MA2HU)
Validated Data
The Binding Activity of Human IGF1R with Anti-IGF1R Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human IGF1R (CSB-MP011087HU1) at 2 μg/mL can bind Anti-IGF1R recombinant antibody. The EC50 is 1.479-1.702 ng/mL.
IHC image of CSB-RA011087MA2HU diluted at 1:50 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA011087MA2HU diluted at 1:50 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of MCF-7 cell with CSB-RA011087MA2HU at 1:30 ,counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific.
Immunofluorescence staining of SH-SY5Y cell with CSB-RA011087MA2HU at 1:30 ,counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific.
Overlay Peak curve showing Hela cells stained with CSB-RA011087MA2HU (red line) at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35 min at 4℃.Control antibody (green line) was mouse IgG2a (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
The following IGF1R
reagents supplied by CUSABIO are manufactured under a
strict quality control system. Multiple applications
have been validated and solid technical support is
offered.
