HMGB1

HMGB1, also known as High Mobility Group Box 1 protein, is a highly conserved nuclear protein. It is also commonly referred to as amphoterin. HMGB1 can act as a damage - associated molecular pattern (DAMP) molecule. When released into the extracellular space, it binds to receptors such as RAGE and TLRs, triggering intracellular signaling cascades.
Involving in multiple signaling pathways like NF - κB and MAPK, HMGB1 has been linked to various diseases, including inflammation, cancer, and neurodegenerative disorders. In drug development, targeting HMGB1 is considered a potential therapeutic strategy. Several inhibitors are under pre - clinical and clinical investigations to mitigate its harmful effects.

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

HMGB1 Recombinant Monoclonal Antibody(CSB-RA439052A0HU)

Validated Data

Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293T whole cell lysate, MCF-7 whole cell lysate, HL60 whole cell lysate
All lanes: HMGB1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa

IHC image of CSB-RA439052A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Human High mobility group protein B1,HMGB-1 ELISA Kit(CSB-E08223h)

Validated Data

Code: CSB-E08223h

Size: 96T,5×96T,10×96T

Sensitivity: 19.5 pg/mL

Detection Range: 78 pg/mL-5000 pg/mL

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

The following HMGB1 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

HMGB1 Antibodies

HMGB1 Proteins

HMGB1 ELISA Kits

HMGB1 Antibodies

HMGB1 Antibodies for Gallus gallus (Chicken)

Code	Product Name	Species Reactivity	Application
CSB-PA590934XA01CH	HMGB1 Antibody	Gallus gallus (Chicken)	ELISA, WB (ensure identification of antigen)

HMGB1 Antibodies for Rattus norvegicus (Rat)

Code	Product Name	Species Reactivity	Application
CSB-PA010553NA01RA	Hmgb1 Antibody	Rat	ELISA, WB
CSB-PA010553NB01RA	Hmgb1 Antibody, HRP conjugated	Rat	ELISA
CSB-PA010553NC01RA	Hmgb1 Antibody, FITC conjugated	Rat
CSB-PA010553ND01RA	Hmgb1 Antibody, Biotin conjugated	Rat	ELISA
CSB-PA010553NA01MO	Hmgb1 Antibody	Rat	ELISA, WB

HMGB1 Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA01604A0Rb	HMGB1 Antibody	Human, Mouse	ELISA, WB, IHC
CSB-PA01604B0Rb	HMGB1 Antibody, HRP conjugated	Human	ELISA
CSB-PA01604C0Rb	HMGB1 Antibody, FITC conjugated	Human
CSB-PA01604D0Rb	HMGB1 Antibody, Biotin conjugated	Human	ELISA
CSB-PA010553GA01HU	HMGB1 Antibody	Human,Mouse,Rat	ELISA,WB,IHC
CSB-PA049959	HMGB1 Antibody	Human,Mouse,Rat	ELISA,WB
CSB-PA000149	Acetyl-HMGB1 (K12) Antibody	Human,Mouse,Rat	WB, ELISA
CSB-PA002937	HMGB1 Antibody	Human,Mouse,Rat	WB, IHC, IF, ELISA
CSB-PA005927	HMGB1 Antibody	Human,Mouse,Rat	WB, ELISA
CSB-PA995991	HMGB1 Antibody	Human,Mouse,Rat	ELISA,IHC
CSB-PA225274	HMGB1 Antibody	Human,Mouse,Rat	ELISA,WB,IHC
CSB-PA010553YA01HU	HMGB1 Antibody	Human, Mouse	ELISA, WB, IHC, IF
CSB-PA010553YB01HU	HMGB1 Antibody, HRP conjugated	Human	ELISA
CSB-PA010553YC01HU	HMGB1 Antibody, FITC conjugated	Human
CSB-PA010553YD01HU	HMGB1 Antibody, Biotin conjugated	Human	ELISA
CSB-RA439052A0HU	HMGB1 Recombinant Monoclonal Antibody	Human	ELISA, WB, IHC
CSB-RA010553MA1HU	HMGB1 Recombinant Monoclonal Antibody	Human	ELISA, IHC, IF, FC

HMGB1 Antibodies for Arabidopsis thaliana (Mouse-ear cress)

Code	Product Name	Species Reactivity	Application
CSB-PA523735XA01DOA	HMGB1 Antibody	Arabidopsis thaliana	ELISA, WB (ensure identification of antigen)

HMGB1 Antibodies for Mus musculus (Mouse)

Code	Product Name	Species Reactivity	Application
CSB-PA010553ZA01MO	Hmgb1 Antibody	Mus musculus	ELISA, WB (ensure identification of antigen)

HMGB1 Proteins

HMGB1 Proteins for Plecturocebus moloch (Dusky titi monkey) (Callicebus moloch)

Code	Product Name	Source
CSB-YP541463CYE CSB-EP541463CYE CSB-BP541463CYE CSB-MP541463CYE CSB-EP541463CYE-B	Recombinant Callicebus moloch High mobility group protein B1 (HMGB1)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

HMGB1 Proteins for Equus caballus (Horse)

Code	Product Name	Source
CSB-YP600961HO CSB-EP600961HO CSB-BP600961HO CSB-MP600961HO CSB-EP600961HO-B	Recombinant Horse High mobility group protein B1 (HMGB1)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

HMGB1 Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP010553HU	Recombinant Human High mobility group protein B1 (HMGB1)	Yeast
CSB-YP010553HU CSB-EP010553HU CSB-BP010553HU CSB-MP010553HU CSB-EP010553HU-B	Recombinant Human High mobility group protein B1 (Hmgb1)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-RP016044h	Recombinant Human High mobility group protein B1 (HMGB1), partial	E.coli
CSB-EP010553MO1	Recombinant Mouse High mobility group protein B1 (HMGB1), partial	E.coli
CSB-MP010553HU	Recombinant Human High mobility group protein B1 (HMGB1)	Mammalian cell
CSB-MP010553HU1	Recombinant Human High mobility group protein B1 (HMGB1), partial	Mammalian cell
CSB-MP010553HU(A4)	Recombinant Human High mobility group protein B1 (HMGB1)	Mammalian cell

HMGB1 Proteins for Sus scrofa (Pig)

Code	Product Name	Source
CSB-YP010553PI CSB-EP010553PI CSB-BP010553PI CSB-MP010553PI CSB-EP010553PI-B	Recombinant Pig High mobility group protein B1 (HMGB1)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-EP010553PI	Recombinant Pig High mobility group protein B1 (HMGB1)	E.coli

HMGB1 Proteins for Bos taurus (Bovine)

Code	Product Name	Source
CSB-YP010553BO CSB-EP010553BO CSB-BP010553BO CSB-MP010553BO CSB-EP010553BO-B	Recombinant Bovine High mobility group protein B1 (HMGB1)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

HMGB1 Proteins for Papio anubis (Olive baboon)

Code	Product Name	Source
CSB-YP434996ERI CSB-EP434996ERI CSB-BP434996ERI CSB-MP434996ERI CSB-EP434996ERI-B	Recombinant Papio anubis High mobility group protein B1 (HMGB1)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

HMGB1 Proteins for Callithrix jacchus (White-tufted-ear marmoset)

Code	Product Name	Source
CSB-YP533334CYL CSB-EP533334CYL CSB-BP533334CYL CSB-MP533334CYL CSB-EP533334CYL-B	Recombinant Callithrix jacchus High mobility group protein B1 (HMGB1)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

HMGB1 Proteins for Canis lupus familiaris (Dog) (Canis familiaris)

Code	Product Name	Source
CSB-YP751094DO CSB-EP751094DO CSB-BP751094DO CSB-MP751094DO CSB-EP751094DO-B	Recombinant Dog High mobility group protein B1 (HMGB1)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

HMGB1 Proteins for Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)

Code	Product Name	Source
CSB-YP362058DXU CSB-EP362058DXU CSB-BP362058DXU CSB-MP362058DXU CSB-EP362058DXU-B	Recombinant Cricetulus griseus High mobility group protein B1 (HMGB1)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

HMGB1 Proteins for Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Code	Product Name	Source
CSB-YP703137MOV CSB-EP703137MOV CSB-BP703137MOV CSB-MP703137MOV CSB-EP703137MOV-B	Recombinant Macaca fascicularis High mobility group protein B1 (HMGB1)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

HMGB1 Proteins for Rattus norvegicus (Rat)

Code	Product Name	Source
CSB-YP010553RA CSB-EP010553RA CSB-BP010553RA CSB-MP010553RA CSB-EP010553RA-B	Recombinant Rat High mobility group protein B1 (Hmgb1)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-EP010553RA	Recombinant Rat High mobility group protein B1 (Hmgb1)	E.coli
CSB-YP010553RA	Recombinant Rat High mobility group protein B1 (Hmgb1)	Yeast
CSB-YP010553RAa1	Recombinant Rat High mobility group protein B1 (Hmgb1)	Yeast

HMGB1 Proteins for Arabidopsis thaliana (Mouse-ear cress)

Code	Product Name	Source
CSB-YP523735DOA CSB-EP523735DOA CSB-BP523735DOA CSB-MP523735DOA CSB-EP523735DOA-B	Recombinant Arabidopsis thaliana High mobility group B protein 1 (HMGB1)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

HMGB1 Proteins for Mus musculus (Mouse)

Code	Product Name	Source
CSB-YP010553MO CSB-EP010553MO CSB-BP010553MO CSB-MP010553MO CSB-EP010553MO-B	Recombinant Mouse High mobility group protein B1 (Hmgb1)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-EP010553MO	Recombinant Mouse High mobility group protein B1 (Hmgb1), partial	E.coli
CSB-YP010553MO	Recombinant Mouse High mobility group protein B1 (Hmgb1), partial	Yeast

HMGB1 ELISA Kit

HMGB1 ELISA Kit for Canis lupus familiaris (Dog) (Canis familiaris)

Code	Product Name	Sample Types	Sensitivity
CSB-EL010553DO	Dog High mobility group protein B1(HMGB1) ELISA kit	serum, plasma, tissue homogenates, cell lysates	0.078 ng/mL

HMGB1 ELISA Kit for Rattus norvegicus (Rat)

Code	Product Name	Sample Types	Sensitivity
CSB-E08224r	Rat High mobility group protein B1,HMGB-1 ELISA Kit	serum, plasma, tissue homogenates	0.39 ng/ml

HMGB1 ELISA Kit for Homo sapiens (Human)

Code	Product Name	Sample Types	Sensitivity
CSB-E08223h	Human High mobility group protein B1,HMGB-1 ELISA Kit	serum, plasma, tissue homogenates, cell lysates	19.5 pg/mL

HMGB1 ELISA Kit for Sus scrofa (Pig)

Code	Product Name	Sample Types	Sensitivity
CSB-EL010553PI	Pig High mobility group protein B1(HMGB1) ELISA kit	serum, plasma, tissue homogenates	0.078 ng/mL

HMGB1 ELISA Kit for Mus musculus (Mouse)

Code	Product Name	Sample Types	Sensitivity
CSB-E08225m	Mouse High mobility group protein B1,HMGB-1 ELISA Kit	serum, plasma, tissue homogenates, cell lysates	3.9 pg/mL

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HMGB1

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