FTH1, Ferritin Heavy Chain 1, alias FHC, FTH, FTHL6, HFE5, PIG15, PLIF and so on. It is widely derived from animal and plant cells and is the main component of ferritin.
FTH1 can catalyze iron ions in the body into Fe3+ and store them to maintain normal iron homeostasis; it has ferrous oxidase activity, which promotes iron oxidation and cellular uptake of iron; it also possesses an anti-oxidative stress function, which is capable of degrading free radicals and effectively preventing oxidative damage caused by excess iron. FTH1 is involved in iron metabolism, including iron absorption, transport and utilization, and also participates in physiological processes such as immune response and cell proliferation, and has a protective effect on cells.
FTH1 is also associated with the development of various diseases, such as iron overload, neurodegenerative diseases, etc. Therefore, the regulation of FTH1 expression may be a potential therapeutic target for various diseases.
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Recombinant Human Epidermal growth factor receptor (EGFR), partial (Active)(CSB-MP007479HU)
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result of CSB-MP007479HU could indicate that this peptide derived from Mammalian Cell-expressed Homo sapiens (Human) EGFR.
Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result of CSB-MP007479HU could indicate that this peptide derived from Mammalian Cell-expressed Homo sapiens (Human) EGFR.
Measured by its binding ability in a functional ELISA. Immobilized EGFR at 1 μg/ml can bind Anti-EGFR recombinant antibody, the EC50 of human EGFR protein is 2.867-3.571 ng/ml.
Human EGF protein captured on COOH chip can bind Human EGFR protein, his and Myc tag (CSB-MP007479HU) with an affinity constant of 11.9nM as detected by LSPR Assay.
Activity
Measured by its binding ability in a functional ELISA. Immobilized EGFR at 1 μg/ml can bind Cetuximab, the EC50 of human EGFR protein is 0.6919-1.047 ng/ml.
The purity of EGFR was greater than 95% as determined by SEC-HPLC
EGFR Recombinant Monoclonal Antibody(CSB-RA159341MA1HU)
Validated Data
IHC image of CSB-RA159341MA1HU diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA159341MA1HU diluted at 1:50 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing NIH/3T3 cells stained with CSB-RA159341MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Rabbit IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
The following FTH1
reagents supplied by CUSABIO are manufactured under a
strict quality control system. Multiple applications
have been validated and solid technical support is
offered.
FTH1 Proteins
FTH1 Proteins for Equus caballus (Horse)
FTH1 Proteins for Homo sapiens (Human)
FTH1 Proteins for Sus scrofa (Pig)
FTH1 Proteins for Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
FTH1 Proteins for Bos taurus (Bovine)
FTH1 Proteins for Canis lupus familiaris (Dog) (Canis familiaris)
FTH1 Proteins for Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
FTH1 Proteins for Ovis aries (Sheep)
FTH1 Proteins for Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
FTH1 Proteins for Felis catus (Cat) (Felis silvestris catus)
FTH1 Proteins for Trichosurus vulpecula (Brush-tailed possum)
FTH1 Proteins for Rattus norvegicus (Rat)
FTH1 Proteins for Oryctolagus cuniculus (Rabbit)
FTH1 Proteins for Mus musculus (Mouse)
