CEACAM5

CEACAM5, carcinoembryonic antigen cell adhesion molecule 5, alias CD66e, is encoded by the human CEACAM5 gene, which is located on chromosome 19q13.2, with an approximate gene length of 21 kilobases (kb), and consists of nine exons and three non-coding exons. CEACAM5 binds to membranes via glycosylphosphatidylinositol (GPI) and has a total of seven extracellular immunoglobulin-like structural domains, including one variable (IgV)-like structural domain and six IgC-like structural domains (A1-B3).
CEACAM5 plays a role in a variety of biological processes, including cell adhesion, cell signaling, and immune regulation, and is highly expressed in many malignant tumor cells, and is widely used for cancer diagnosis and monitoring. Aberrant expression of CEACAM5 is associated with the development of many cancers, especially overexpressed in colorectal, gastric, lung and pancreatic cancers, and is a potential target for cancer therapy.

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CEACAM5 at 2μg/mL can bind Anti-CEACAM5 recombinant antibody (CSB-RA005165MA1HU), the EC₅₀ is 0.8955-1.719 ng/mL.

Recombinant Macaca mulatta Carcinoembryonic antigen-related cell adhesion molecule 5(CEACAM5),partial (Active)(CSB-MP5152MOW)

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus CEACAM5 at 2μg/ml can bind Anti-CEACAM5 recombinant antibody (CSB-RA005165MA4HU). The EC₅₀ is 3.572-4.044 ng/mL.

The purity of CEACAM5 was greater than 95% as determined by SEC-HPLC

CEACAM5 Recombinant Monoclonal Antibody(CSB-RA005165MA3HU)

Validated Data

Immunofluorescence staining of Hela cell with CSB-RA005165MA3HU at 1:150, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-human IgG(H+L).

Immunofluorescence staining of MCF7 cell with CSB-RA005165MA3HU at 1:150, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-human IgG(H+L).

Overlay Peak curve showing Hela cells surface stained with CSB-RA005165MA3HU (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10⁶cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was human IgG1 (1µg/1*10⁶cells) used under the same conditions. Acquisition of >10,026 events was performed.

The following CEACAM5 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

CEACAM5 Antibodies

CEACAM5 Proteins

CEACAM5 ELISA Kits

CEACAM5 Antibodies

CEACAM5 Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA005033	CEACAM5 Antibody	Human	WB, ELISA
CSB-PA160116	CEACAM5 Antibody	Human	ELISA,WB,IHC
CSB-PA229032	CEACAM5 Antibody	Human	ELISA,WB,IHC
CSB-PA109108	CEACAM5 Antibody	Human	ELISA,IHC
CSB-PA097333	CEACAM5 Antibody	Human	ELISA,IHC
CSB-PA005165LA01HU	CEACAM5 Antibody	Human	ELISA, WB, IHC, IF
CSB-PA005165LB01HU	CEACAM5 Antibody, HRP conjugated	Human	ELISA
CSB-PA005165LC01HU	CEACAM5 Antibody, FITC conjugated	Human
CSB-PA005165LD01HU	CEACAM5 Antibody, Biotin conjugated	Human	ELISA
CSB-MA000218	CEACAM5 Monoclonal Antibody	Human	ELISA,IHC
CSB-MA995583	CEACAM5 Monoclonal Antibody	Human	ELISA, IHC
CSB-RA005165MA2HU	CEACAM5/CEACAM6 Recombinant Monoclonal Antibody	Human	ELISA
CSB-RA005165MA3HU	CEACAM5 Recombinant Monoclonal Antibody	Human	ELISA, IF, FC
CSB-RA005165MA1HU	CEACAM5 Recombinant Monoclonal Antibody	Human	ELISA
CSB-RA005165MA4HU	CEACAM5 Recombinant Monoclonal Antibody	Human, Cynomolgus	ELISA
CSB-PA005165XA01HU	CEACAM5 Antibody	Homo sapiens (Human)	ELISA, WB (ensure identification of antigen)
CSB-RA005165MB1HU	CEACAM5/CEACAM6 (NEO-201 Biosimilar) Recombinant Monoclonal Antibody	Human
CSB-RA005165MB2HU	CEACAM5 (labetuzumab Biosimilar) Recombinant Monoclonal Antibody	Human
CSB-RA005165MB3HU	CEACAM5 (Tusamitamab Biosimilar) Recombinant Monoclonal Antibody	Human

CEACAM5 Proteins

CEACAM5 Proteins for

Code	Product Name	Source
CSB-MP005165HUd9	Recombinant Human Cell adhesion molecule CEACAM5 (CEACAM5), partial	Mammalian cell

CEACAM5 Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP005165HU	Recombinant Human Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) (E398K)	Yeast
CSB-MP005165HU	Recombinant Human Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) (E398K) (Active)	Mammalian cell
CSB-MP005165HU(M)-B	Recombinant Human Cell adhesion molecule CEACAM5 (CEACAM5) (E398K), Biotinylated	Mammalian cell

CEACAM5 Proteins for Macaca mulatta (Rhesus macaque)

Code	Product Name	Source
CSB-MP5152MOW	Recombinant Macaca mulatta Carcinoembryonic antigen-related cell adhesion molecule 5(CEACAM5),partial (Active)	Mammalian cell

CEACAM5 Proteins for Mus musculus (Mouse)

Code	Product Name	Source
CSB-YP005165MO CSB-EP005165MO CSB-BP005165MO CSB-MP005165MO CSB-EP005165MO-B	Recombinant Mouse Carcinoembryonic antigen-related cell adhesion molecule 5 (Ceacam5), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-MP005165MO(A4)	Recombinant Mouse Carcinoembryonic antigen-related cell adhesion molecule 5 (Ceacam5)	Mammalian cell
CSB-MP005165MO	Recombinant Mouse Carcinoembryonic antigen-related cell adhesion molecule 5 (Ceacam5)	Mammalian cell

CEACAM5 ELISA Kit

CEACAM5 ELISA Kit for Homo sapiens (Human)

Code	Product Name	Sample Types	Sensitivity
CSB-E04767h	Human carcinoembryonic antigen,CEA ELISA Kit	serum, plasma, tissue homogenates	2 ng/mL

CEACAM5 ELISA Kit for Mus musculus (Mouse)

Code	Product Name	Sample Types	Sensitivity
CSB-E13925m	Mouse carcinoembryonic antign,CEA ELISA Kit	serum, plasma, tissue homogenates	15.6 pg/mL

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CEACAM5

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