CEACAM5/CEACAM6 Recombinant Monoclonal Antibody

Code: CSB-RA005165MA2HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA

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Target Background

Product Details

Uniprot NO.

P06731&P40199

Target Names

CEACAM5/CEACAM6

Alternative Names

Carcinoembryonic antigen antibody; Carcinoembryonic antigen-related cell adhesion molecule 5 antibody; CD66e antibody; CEA antibody; Ceacam5 antibody; CEAM5_HUMAN antibody; DKFZp781M2392 antibody; Meconium antigen 100 antibody; OTTHUMP00000199032 antibody; OTTHUMP00000199033 antibody; OTTHUMP00000199034 antibody;Carcinoembryonic antigen related cell adhesion molecule 6 antibody; Carcinoembryonic antigen related cell adhesion molecule 6 (non specific cross reacting antigen) antibody; Carcinoembryonic antigen-related cell adhesion molecule 6 antibody; CD 66c antibody; CD66c antibody; CD66c antigen antibody; CEA LIKE PROTEIN antibody; CEACAM 6 antibody; CEACAM6 antibody; CEAL antibody; CEAM6_HUMAN antibody; MGC93832 antibody; NCA antibody; Non specific cross reacting antigen antibody; Non-specific crossreacting antigen antibody; Normal cross reacting antigen antibody; Normal cross-reacting antigen antibody

Species Reactivity

Human

Immunogen

Recombinant Human CEACAM5/CEACAM6 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

11A1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA

Protocols

ELISA Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

The Binding Activity of Human CEACAM5 with Anti-CEACAM5/CEACAM6 Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CEACAM5 at 2μg/mL can bind Anti-CEACAM5/CEACAM6 recombinant antibody (CSB-RA005165MA2HU), the EC₅₀ is 0.4282-1.151 ng/mL.
The Binding Activity of Human CEACAM6 with Anti- CEACAM5/CEACAM6 recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CEACAM6 (CSB-MP005166HU) at 2 μg/mL can bind Anti- CEACAM5/CEACAM6 recombinant antibody, the EC₅₀ is 0.9430-1.377 ng/mL.

Description

CUSABIO undertook a stepwise approach to produce the CEACAM5/CEACAM6 recombinant monoclonal antibody. Initially, B cells were isolated from the spleen of an immunized animal, utilizing recombinant human CEACAM5/CEACAM6 protein as the immunogen during the immunization process. Subsequently, RNA was extracted from the B cells and reversely transcribed into cDNA. Using the cDNA as a template, the gene encoding the CEACAM5/CEACAM6 antibody was extended with a degenerate primer and inserted into a vector. This recombinant vector was then transfected into host cells to enable antibody expression. The CEACAM5/CEACAM6 recombinant monoclonal antibodies were harvested from the cell culture supernatant and purified via affinity chromatography. To validate this antibody's specificity, it underwent testing in ELISA to confirm its reactivity with human CEACAM5 and CEACAM6 proteins.