CALR encodes calreticulin, also known as CRP55, calregulin, and HACBP. This multifunctional calcium-binding chaperone protein resides mainly in the endoplasmic reticulum (ER). It aids in protein folding, maintains calcium homeostasis, and participates in the immune response. It is involved in the unfolded protein response and MHC class I antigen presentation pathways.
Mutations in CALR are strongly associated with myeloproliferative neoplasms like essential thrombocythemia and primary myelofibrosis. Regarding drug development, researchers are exploring targeted therapies to inhibit the abnormal effects caused by CALR mutations, aiming to provide new treatment options for these related diseases.
Popular
Product:
CALR Recombinant Monoclonal Antibody(CSB-RA004458MA1HU)
Validated Data
Western Blot
Positive WB detected in: 293 whole cell lysate
All lanes: CALR antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
IHC image of CSB-RA004458MA1HU diluted at 1:300 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of HepG2 cell with CSB-RA004458MA1HU at 1:250, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Overlay Peak curve showing JK cells stained with CSB-RA004458MA1HU (red line) at 1:300. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,018 events was performed.
The following CALR
reagents supplied by CUSABIO are manufactured under a
strict quality control system. Multiple applications
have been validated and solid technical support is
offered.
CALR Proteins
CALR Proteins for Canis lupus familiaris (Dog) (Canis familiaris)
CALR Proteins for Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
CALR Proteins for Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
CALR Proteins for Rattus norvegicus (Rat)
CALR Proteins for Homo sapiens (Human)
CALR Proteins for Oryctolagus cuniculus (Rabbit)
CALR Proteins for Macaca fuscata fuscata (Japanese macaque)
CALR Proteins for Sus scrofa (Pig)
CALR Proteins for Bos taurus (Bovine)
CALR Proteins for Mus musculus (Mouse)
CALR Proteins for Chlorocebus aethiops (Green monkey) (Cercopithecus aethiops)
