Recombinant Rat Calreticulin (Calr)

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Calr

Uniprot NO.

P18418

Species

Rattus norvegicus (Rat)

Source

Yeast

Expression Region

18-416

Target Protein Sequence

DPA IYFKEQFLDG DAWTNRWVES KHKSDFGKFV LSSGKFYGDQ EKDKGLQTSQ DARFYALSAR FEPFSNKGQT LVVQFTVKHE QNIDCGGGYV KLFPGGLDQK DMHGDSEYNI MFGPDICGPG TKKVHVIFNY KGKNVLINKD IRCKDDEFTH LYTLIVRPDN TYEVKIDNSQ VESGSLEDDW DFLPPKKIKD PDAAKPEDWD ERAKIDDPTD SKPEDWDKPE HIPDPDAKKP EDWDEEMDGE WEPPVIQNPE YKGEWKPRQI DNPDYKGTWI HPEIDNPEYS PDANIYAYDS FAVLGLDLWQ VKSGTIFDNF LITNDEAYAE EFGNETWGVT KAAEKQMKDK QDEEQRLKEE EEDKKRKEEE EAEDKEDEDD RDEDEDEEDE KEEDEEDATG QAKDEL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis. Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy.

Gene References into Functions

CRT might protect cardiomyocytes from apoptosis induced by angiotensin II, in which ER stress and mitochondria function are possible underlying molecular bases PMID:29453988
Exogenous CRT attenuates MR-induced ER stress-related apoptosis by suppressing CHOP-mediated apoptotic signaling pathways in MMECs. PMID:24589181
Data highlights the potential importance of CRT and other endoplasmic reticulum stress proteins in inflammatory demyelination PMID:24252779
These data demonstrate that CRT is localized at cardiomyocyte mitochondria and its mitochondrial content is increased in DCM hearts. PMID:24813996
Data suggest that Wistar Kyoto rats are unlike humans in that plasma calreticulin is not up-regulated during pregnancy in rats. Placental calreticulin is not altered by fetal growth retardation in either species (uterine vessel ligation rat model). PMID:23972286
found that at 7 days of single-prolonged stress rats had the highest calreticulin expression PMID:23575574
CRT-regulated Ca(2+)-dependent pathways are a critical molecular link between ER stress and TGF-beta fibrotic signaling PMID:23564462
The level of CRT is enhanced in nerve growth factor-stimulated differentiation of PC-12 cells through extracellular signal-regulated kinase (ERK)-dependent mitogen-activated protein kinase (MAPK) pathway. PMID:22147490
Calreticulin exits the endoplasmic reticulum (ER) to accumulate in the ER-Golgi intermediate compartment and the cis-Golgi, together with sub-optimally loaded H-2Db histocompatibility class I antigen molecules. PMID:19851281
CRT P-domain fragment CRT(221-256) constitutes an autonomous folding unit, and has a structure highly similar to that of the corresponding region in CRT(189-288) PMID:12270713
may represent a new class of high-affinity melatonin binding sites involved in some functions of the indoleamine including genomic regulation PMID:12603316
Calreticulin inhibits the MEK1,2-ERK1,2 pathway in alpha 1-adrenergic receptor/Gh-stimulated hypertrophy of neonatal rat cardiomyocytes PMID:12648529
Data show that calreticulin has an independent role in the cytosol that depends critically on its inefficient compartmentalization due to the signal sequence. PMID:16198296
CRT arginylation occurs in the cytosolic pool of mature CRT (defined by an Asp acid N-terminal) that is probably retrotranslocated from the endoplasmic reticulum PMID:17197444
Calreticulin is a high affinity ligand of GABARAP. PMID:17916189
These findings imply an important role of serine dephosphorylation and tyrosine phosphorylation on calreticulin mediated AT1 receptor mRNA stability. PMID:18413143

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Subcellular Location

Endoplasmic reticulum lumen. Cytoplasm, cytosol. Secreted, extracellular space, extracellular matrix. Cell surface. Sarcoplasmic reticulum lumen. Cytoplasmic vesicle, secretory vesicle, Cortical granule. Cytolytic granule.

Protein Families

Calreticulin family

Tissue Specificity

Predentin and odontoblast.

Database Links

UNIGENE: Rn.974

KEGG: rno:64202

STRING: 10116.ENSRNOP00000004091

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