APOE

CSB-MP004928HU is detected by Mouse anti-10*His monoclonal antibody.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CD37 at 10 μg/ml can bind Anti-CD37 recombinant antibody(CSB-RA004928MA1HU). The EC₅₀ is 7.458-9.930 ng/mL.The VLPs (CSB-MP3838) is negative control.

The purity of VLPs was greater than 95% as determined by SEC-HPLC

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human APOE at 2 μg/ml can bind Anti-APOE recombinant antibody (CSB-RA001936MA1HU). The EC₅₀ is 2.574-2.908 ng/mL.

Immunofluorescence staining of HepG2 cells with CSB-PA07599A0Rb at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA07599A0Rb diluted at 1:1000 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Human apolipoprotein E (Apo-E) ELISA Kit