ANXA1, or Annexin A1, alias lipocortin I. ANXA1 is encoded by the human ANXA1 gene, which is localized to chromosome 9q24 and contains 13 exons and 12 introns. ANXA1 is a calcium-dependent phospholipid-binding protein that is a member of the Annexins family, and contains a core region that contains four repeats of a “type 2” calcium-binding domain motif and an N-terminal structural domain. When ANXA1 in the cytoplasm binds to calcium ions, it transfers to the cell membrane and binds to it, thereby exposing the N-terminal structural domain, which is the main functional structural domain of ANXA1, and exerts anti-inflammatory responses and promotes lysis mainly through binding to the formyl peptide receptor, FPR2.
ANXA1 plays a role in a wide range of cellular life activities, including anti-inflammatory responses, cell differentiation and proliferation, cell death signaling regulation, and phagocytic clearance of apoptotic cells.
Popular
Product:
Recombinant Human Annexin A1 (ANXA1), partial(CSB-EP001836HU1)
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-RP134574h could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) ANX1.
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-RP134574h could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) ANX1.
ANXA1 Recombinant Monoclonal Antibody(CSB-RA001836MA1HU)
Validated Data
IHC image of CSB-RA001836MA1HU diluted at 1:30 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing MCF7 cells stained with CSB-RA001836MA1HU (red line) at 1:400. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,016 events was performed.
The following ANXA1
reagents supplied by CUSABIO are manufactured under a
strict quality control system. Multiple applications
have been validated and solid technical support is
offered.
ANXA1 Proteins
ANXA1 Proteins for Pan troglodytes (Chimpanzee)
ANXA1 Proteins for Gallus gallus (Chicken)
ANXA1 Proteins for Cavia cutleri (Guinea pig)
ANXA1 Proteins for Equus caballus (Horse)
ANXA1 Proteins for Rattus norvegicus (Rat)
ANXA1 Proteins for Rodentia sp.
ANXA1 Proteins for Homo sapiens (Human)
ANXA1 Proteins for Oryctolagus cuniculus (Rabbit)
ANXA1 Proteins for Sus scrofa (Pig)
ANXA1 Proteins for Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
ANXA1 Proteins for Bos taurus (Bovine)
ANXA1 Proteins for Mus musculus (Mouse)
