Abbreviation
Recombinant Human IFNW1 protein, partial (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human TF-1 cells is less than 0.01 ng/ml, corresponding to a specific activity of >1.0x108 IU/mg.
Alternative Names
IFNW1; IFNW1_HUMAN; Interferon alpha II 1 ; Interferon alpha-II-1; Interferon omega 1; Interferon omega-1
Species
Homo sapiens (Human)
Expression Region
24-195aa
Target Protein Sequence
CDLPQNHGLL SRNTLVLLHQ MRRISPFLCL KDRRDFRFPQ EMVKGSQLQK AHVMSVLHEM LQQIFSLFHT ERSSAAWNMT LLDQLHTGLH QQLQHLETCL LQVVGEGESA GAISSPALTL RRYFQGIRVY LKEKKYSDCA WEVVRMEIMK SLFLSTNMQE RLRSKDRDLG SS
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
0.2 μm filtered PBS, pH 7.4 ,lyophilized
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
Interferon omega-1 occupies a unique niche within the type I interferon family, sharing antiviral and antiproliferative signaling through the JAK/STAT pathway yet displaying distinct immunomodulatory kinetics compared to IFN-alpha subtypes. This tag-free recombinant human IFNW1 (aa 24–195), expressed in *E. coli*, delivers exceptional potency with an ED50 below 0.01 ng/ml in a TF-1 chemotaxis bioassay, corresponding to a specific activity exceeding 1.0 × 10⁸ IU/mg—providing a reliable reference standard for cytokine detection assays and a robust positive control for cell-based proliferation and activation studies. The endotoxin level of less than 1.0 EU/μg satisfies the stringent criteria required to prevent LPS-driven artifacts in sensitive immune cell functional assays, including PBMC stimulation, JAK/STAT phosphorylation profiling, and antibody validation by ELISA. Purity exceeding 97% by SDS-PAGE, combined with this endotoxin control, supports use in *in vivo* models of infection or tumor biology where dose-attribution confidence is essential.
