Recombinant Rat Synaptotagmin-1 (Syt1)

1. The expression of p-65 is up-regulated in dorsal root ganglion following surgical incision compared to control groups. PMID:29115943
2. Study showed postsynaptic localization of synaptotagmin 1, at concentrations moderately lower than, but comparable to presynaptic concentrations. It is present in significant concentrations at the postsynaptic density, pointing to the likelihood of insertion of glutamate receptors directly into the synaptic plasma membrane. Synaptotagmin 1 is reduced in postsynaptic spines after eight weeks of kainite-induced epilepsy. PMID:28686803
3. The authors show that synaptotagmin-1 (from Rattus norvegicus and expressed in Escherichia coli) binds to phosphatidylinositol 4,5-diphosphate via a polybasic lysine patch in the C2B domain, which may promote the priming or docking of synaptic vesicles. PMID:27791979
4. In this study, the authors show that the C2B domain of Syt1 interacts simultaneously with acidic membranes and SNARE complexes via the top Ca(2+)-binding loops, the side polybasic patch, and the bottom face in response to Ca(2+). PMID:27083046
5. Data suggest that GTP-binding protein beta/gamma (Gb/g) heterodimers interact specifically with lipid-embedded neuronal t-SNARE proteins syntaxin 1A and SNAP-25B (synaptosomal-associated protein 25B); this binding is competitive with synaptotagmin 1 for binding sites on the t-SNARE proteins; Gb/g inhibits Ca2+/synaptotagmin 1-dependent membrane fusion. PMID:28515322
6. After single synaptic vesicle fusion, syt1 acted as an essential determinant of synaptic vesicle endocytosis time course by delaying the kinetics of vesicle retrieval in response to increasing Ca(2+) levels. PMID:28111077
7. Substitution of 4 Syt1 residues, YHRD, at C2 domain interface, disrupted interaction between tandem C2 domains, altered intrinsic affinity of Syt1 for Ca(2+), and shifted Ca(2+) dependency for binding to membranes and driving membrane fusion. YHRD mutant yielded reductions in synaptic transmission in neurons. Thus physical interactions between tandem C2 domains of syt1 contribute to excitation-secretion coupling. PMID:26792839
8. Study indicates that the accumulation of Synaptotagmin1 may play an important role in axon/dendrite differentiation PMID:26667128
9. different structural states of syt underlie the control of distinct forms of synaptic transmission. PMID:27001899
10. The interaction of Dvl1 with Syt-1, which is regulated by Wnts, modulates neurotransmitter release. PMID:26400647
11. Syt-1 regulates endogenous APP-CTF and Abeta levels in PC12 cells. Syt-1 knockdown reduces APP-CTF, sAPPbeta and Abeta levels. Secreted sAPPbeta levels were significantly reduced in PC12 cells lacking Syt-1 expression. PMID:26202512
12. Membrane interaction of synaptotagmin-1 rather than SNARE binding triggers exocytosis of vesicles. PMID:26389740
13. NMR approach reveals a dynamic binding mode in which basic residues in the concave side of the Syt1 C2B-domain beta-sandwich interact with a polyacidic region of the SNARE complex formed by syntaxin-1 and SNAP-25. PMID:26030874
14. Modifications in the 60-residue linker modulate both the oligomerization of Syt1 and its interaction with opposing bilayers. In addition, the oligomerization of Syt1 may play a role in organizing proteins within the active zone of membrane fusion. PMID:24973220
15. Data suggest that due to the intrinsic copper binding properties of the protein, a pre-labelling approach is preferred for the C2Ac domain of synaptotagmin I when copper is the desired radioisotope. PMID:23954480
16. Analysis of SNARE complex/synaptotagmin-1 interactions by one-dimensional NMR Spectroscopy PMID:23617808
17. the flexible linker region of Syt1 undergoes conformational changes during vesicle fusion: it stretches out to mediate vesicle docking, but folds to assist the C2AB domain for fusion pore opening. PMID:24001110
18. Down-regulation of synaptotagmin 1 in cortex, hippocampus, and cerebellum is associated with experimental subarachnoid hemorrhage. PMID:23884218
19. Iodine deficiency and propylthiouracil treatment reduce synaptotagmin-1 and PSD-95 in the rat hippocampus. PMID:23321072
20. Using PC12 cells from Rattus norvegicus and artificial supported bilayers, authors show that synaptotagmin-1 interacts with the polybasic linker region of syntaxin-1A independent of Ca(2+) through phosphatidylinositol 4,5-bisphosphate. PMID:23665582
21. This study demonistrated that synaptotagmin 11 distribution in neuron of rat hippocampus and presynapse and postsynapse. PMID:22960622
22. Synaptotagmin I regulates patterned spontaneous activity in the developing rat retina via calcium binding to the C2AB domains. PMID:23091625
23. It appears that SNAREs alone are sufficient in promoting membrane hemifusion, although Syt1 and Ca2+ are required to carry over toward pore formation and expansion. PMID:23300284
24. synaptotagmin isoforms localize to distinct secretory organelles in both axons and dendrites and may regulate neuropeptide/neurotrophin release to modulate neuronal function. PMID:22398727
25. vesicles have a threshold for syt I that is required to control opening of the fusion pore, expansion, and full fusion to release large dense core proteins, but not for full fusion of small molecules PMID:22609930
26. Trans-interactions of synaptotagmin considered to be essential for its function are controlled by a delicate balance between cis- and trans-binding, which may play an important modulatory role in synaptic transmission. PMID:22711810
27. Dysregulation of SNARE complex and syt-1 in prefrontal cortex of adult-onset hypothyroidism can be restored by T(4) treatment. PMID:21646859
28. PIP(2) makes synaptotagmin-1 >40-fold more sensitive to Ca(2+). This interplay between Ca(2+), synaptotagmin-1, and PIP(2) is crucial for neurotransmitter release. PMID:22447935
29. for Syt1 to function as a Ca2+ sensor, a charge asymmetry appears to be important and this may play a role in steering Syt1 to productively trans bind to the plasma membrane PMID:22229667
30. Synaptotagmin I binds to syntaxin 1 electrostatically through C(2)B domain effector region in a Ca(2+)-independent fashion, providing biochemical evidence that synaptotagmin I binds SNARE complexes before Ca(2+) influx into presynaptic nerve terminals. PMID:22008253
31. syt1 might facilitate SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptors)-mediated membrane fusion by phase separating PS PMID:21610074
32. These results suggest a conserved lipid binding mechanism in which Ca(2+)-independent interactions are mediated via a lysine rich region of the C2B domain while Ca(2+)-dependent interactions are mediated via the Ca(2+)-binding loops. PMID:21928778
33. Data suggest that immature medial nucleus of the trapezoid body terminals may contain two populations of synaptic vesicles, one expressing VGLUT3 with synaptotagmin 2 and another expressing VGLUT3 with synaptotagmin 1. PMID:21456023
34. Immunocytochemistry in rat chromaffin cells indicated that Syt1 was localized in large dense-core vesicles and synaptic-like microvesicles, whereas Syt7 was the predominant isoform present in large dense-core vesicles. PMID:21287204
35. investigation of interaction of synaptotagmin 1 (and it's domains) with membrane bilayer PMID:21344950
36. lipid-binding properties of 2 domains of Syt1: C2A domain selectively binds to disordered domains; C2B domain selectively binds to ordered domains PMID:21322640
37. This analysis showed that C2AB domain of synaptotagmin and Ca(2+) accelerate vesicle-vesicle docking with more than two orders of magnitude. PMID:21320440
38. The Syt7 C2B domain structure is very similar to that of the Syt1 C2B domain and contains three Ca2+-binding sites. PMID:20824061
39. Data show that Syt I produced more rapid dilation of fusion pores than syt VII or syt IX, consistent with its role in synchronous synaptic release. PMID:20573977
40. The results suggest that when associated with the SNAREs, syt1 is configured to bind opposing bilayers, but that the syt1/SNARE complex samples multiple conformational states. PMID:21087613
41. the interplay between tomosyn and synaptotagmin-1 underlies inhibitory control of Ca(2+)-dependent neurotransmitter release. PMID:20978127
42. These data support an inhibitory effect of syt IV on release of vesicles and their transmitter content which became more pronounced when syt I expression was abolished PMID:20735850
43. findings show Syt1 requires the membrane anchor to stimulate vesicle fusion at physiological Ca2+ levels and may function as a dynamic presynaptic Ca2+ sensor to control the probability of neurotransmitter release PMID:20448186
44. isolated an SYT1-associated endocytic complex (SAE) from presynaptic nerve terminals and used a novel fractional recovery assay based on electrostatic dissociation to identify SAE components and map the complex structure. PMID:17183698
45. phosphorylation-dependent interaction with cysteine string protein PMID:11931641
46. The InsP6 binding notably weakened the Ca(2+)-dependent C2AB-membrane interaction, which suggests that InsP6 may act as a modulator of neurotransmitter release by altering the state of synaptotagmin-phospholipid interaction. PMID:12220627
47. structure and binding to a truncated neuronal SNARE complex PMID:12496247
48. calcium-independent properties of Syt 1 regulate voltage-gated Ca2+ channels and contribute to the molecular events underlying transmitter release PMID:12645522
49. analysis of palmitoylation sites and processing of synaptotagmin I PMID:12782290
50. The synaptotagmin C2A domain is part of the calcium sensor controlling fast synaptic transmission. PMID:12873386

Show More

Hide All

UNIGENE: Rn.216272

KEGG: rno:25716

STRING: 10116.ENSRNOP00000049624