Recombinant Mouse Tyrosinase (Tyr)

In Stock

Code: CSB-CF025394MO

Rate （5.0）
Review （1）
Citation （1）

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US$1620

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Express system: in vitro E.coli expression system

Species: Mus musculus (Mouse)

Tag Info: N-terminal 10xHis-tagged

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Product Details
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Target Background

Product Details

Abbreviation

Recombinant Mouse Tyr protein

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

Tyr

Uniprot NO.

P11344

Research Area

Others

Species

Mus musculus (Mouse)

Source

in vitro E.coli expression system

Expression Region

19-533aa

Target Protein Sequence

HFPRACASSKNLLAKECCPPWMGDGSPCGQLSGRGSCQDILLSSAPSGPQFPFKGVDDRESWPSVFYNRTCQCSGNFMGFNCGNCKFGFGGPNCTEKRVLIRRNIFDLSVSEKNKFFSYLTLAKHTISSVYVIPTGTYGQMNNGSTPMFNDINIYDLFVWMHYYVSRDTLLGGSEIWRDIDFAHEAPGFLPWHRLFLLLWEQEIRELTGDENFTVPYWDWRDAENCDICTDEYLGGRHPENPNLLSPASFFSSWQIICSRSEEYNSHQVLCDGTPEGPLLRNPGNHDKAKTPRLPSSADVEFCLSLTQYESGSMDRTANFSFRNTLEGFASPLTGIADPSQSSMHNALHIFMNGTMSQVQGSANDPIFLLHHAFVDSIFEQWLRRHRPLLEVYPEANAPIGHNRDSYMVPFIPLYRNGDFFITSKDLGYDYSYLQESDPGFYRNYIEPYLEQASRIWPWLLGAALVGAVIAAALSGLSSRLCLQKKKKKKQPQEERQPLLMDKDDYHSLLYQSHL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

61.3 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Datasheet & COA

Please contact us to get it.

Images

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

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Applications : qPCR analysis

Review: The TYRP2 and Tyr-specific CD8+ T cells and serum anti-TYRP2 and Tyr antibodies were upregulated in AA mice. mRNA (Tyrp2 and Tyr) expressions in the skin analyzed by qPCR.

By Anonymous

Citations

Altered T cell subpopulations and serum anti-TYRP2 and tyrosinase antibodies in the acute and chronic phase of alopecia areata in the C3H/HeJ mouse model K Hashimoto,/,2021

Target Background

Function(From Uniprot)

This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine. In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone.

Gene References into Functions

This study demonstrates the utility of CRISPR-Cas9 system in generating domain-specific in-frame deletions and helps gain further insights into structure-function of Tyr gene. PMID:27224051
These results suggest that the tyrosinase-induced metabolism of rhodendrol causes melanocyte toxicity. PMID:25713930
Data indicate that both ephedrannins A and B were competitive inhibitors of mushroom tyrosinase. PMID:25857772
STAP-2 colocalized with tyrosinase in lysosomes and protected tyrosinase from protein degradation in B16F10 melanoma cells. PMID:26023234
Docosahexaenoic acid inhibits melanogenesis in B16F10 cells in vitro through increased tyrosinase degradation. PMID:24562306
Studied upregulation by Kaliziri extract of tyrosinase, TRP-1, TRP-2 and MITF expression in murine B16 melanoma cells. PMID:24884952
The albino mutation of tyrosinase alters ocular angiogenic responsiveness. PMID:23423728
Tyrosinase is mutated in the albino dorsal lateral geniculate nucleus, arising from retinal ganglion cell misspecification together with potential perturbations of early activity patterns in the albino retina. PMID:22492037
activated by ascorbic acid in B16 melanoma via p38 MAPK signaling and upregulation of melanogenic regulatory factors PMID:21667118
Effect of zibuganshenfang on the expression and activity of tyrosinase on B16 cells PMID:22256768
the schisis phenotype caused by the Rs1 mutation is rescued by a Tyr mutation in the C57BL/6J genetic background, strongly suggesting that Tyr is the Mor1 gene. PMID:20876567
Data suggest that accumulation of tyrosinase in the endoplasmic reticulum of melanocytes leads to ER stress and activation of the unfolded protein response. PMID:20444203
Data show that interfering with p38 MAPK expression stimulates melanogenesis and tyrosinase family protein expression. PMID:20053998
role of P protein and tyrosinase in oculocutaneous albinism PMID:12028586
processing is controlled by pink-eyed dilution protein PMID:12058062
identified the tyrosinase gene (Tyr) as a modifier of the drainage structure phenotype in developmental glaucoma PMID:12624268
Tyrosinase maturation and oligomerization in the endoplasmic reticulum require a melanocyte-specific factor PMID:12724309
investigation of the boundary activity of locus control regions in transgenic animals and cells PMID:14576318
tyrosinase has a role in fatty acid regulation of pigmentation PMID:14739285
Interactions between N-linked glycans and lectin chaperones help to minimize tyrosinase aggregation and also target non-native substrates for retro-translocation and subsequent degradation PMID:15161941
Tyrosinase is a subject to post-Golgi quality control. PMID:15760341
tyrosinase has roles in the development of the visual system, as shown in a transgenic mouse model [review] PMID:16146766
tyrosinase and related proteins as physiological substrates of presenilins and link gamma-secretase activity with intracellular protein transport PMID:16384915
These results therefore suggest that proteasomes, particularly p27 subunit, are directly involved in the regulation of melanin biosynthesis in mouse melanoma cells. PMID:16879986
a 186 kb BAC containing the tyrosinase gene provides transgene expression in both RPE and melanocytes indicating the presence of regulatory sequences required for expression in the RPE PMID:17196956
Gallic acid effectively suppressed Tyr activity. PMID:17541153
The inhibitory effect of 4-hydroxybenzyl alcohol on melanogenesis is due to the direct inhibition of melanosomal Tyr activity, rather than to the suppression of Tyr gene. PMID:17541167
analysis of the mammalian tyrosinase active site with loss of function mutations PMID:17850513
these data demonstrate that Tyrp1 interacts directly with Tyrosinase in vivo, which may regulate the stability and trafficking of melanogenic enzymes and thus pigment synthesis. PMID:18042623
Results describe the molecular detection of circulating melanoma cells by reverse transcription-polymerase chain reaction (RT-PCR) in human blood samples and in a xenograft mouse model. PMID:18425346
the pigment-cell-specific cuproenzyme tyrosinase acquires copper only transiently and inefficiently within the trans-Golgi network of mouse melanocytes PMID:18650808
tyrosinase as a beta1,6 branch containing glycoprotein: substrate of GnT-V PMID:18655794
Data suggest that the tyrosinase promoter may be transiently active around E10 in subsets of precursor cells in the neural crest and intestines. PMID:19220779