Recombinant Mouse Myotonin-protein kinase (Dmpk)

Code: CSB-CF006968MO

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20μg100μg

US$7216

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Express system: in vitro E.coli expression system

Species: Mus musculus (Mouse)

Tag Info: N-terminal 10xHis-tagged

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Target Background

Product Details

Target Names

Dmpk

Uniprot NO.

P54265

Alternative Names

Dmpk; Dm15; Mdpk; Myotonin-protein kinase; MT-PK; DM-kinase; DMK; DMPK; Myotonic dystrophy protein kinase; MDPK

Species

Mus musculus (Mouse)

Source

in vitro E.coli expression system

Expression Region

1-631

Target Protein Sequence

MSAEVRLRQLQQLVLDPGFLGLEPLLDLLLGVHQELGASHLAQDKYVADFLQWVEPIAARLKEVRLQRDDFEILKVIGRGAFSEVAVVKMKQTGQVYAMKIMNKWDMLKRGEVSCFREERDVLVKGDRRWITQLHFAFQDENYLYLVMEYYVGGDLLTLLSKFGERIPAEMARFYLAEIVMAIDSVHRLGYVHRDIKPDNILLDRCGHIRLADFGSCLKLQPDGMVRSLVAVGTPDYLSPEILQAVGGGPGAGSYGPECDWWALGVFAYEMFYGQTPFYADSTAETYAKIVHYREHLSLPLADTVVPEEAQDLIRGLLCPAEIRLGRGGAGDFQKHPFFFGLDWEGLRDSVPPFTPDFEGATDTCNFDVVEDRLTAMVSGGGETLSDMQEDMPLGVRLPFVGYSYCCMAFRDNQVPDPTPMELEALQLPVSDLQGLDLQPPVSPPDQVAEEADLVAVPAPVAEAETTVTLQQLQEALEEEVLTRQSLSRELEAIRTANQNFSSQLQEAEVRNRDLEAHVRQLQERMEMLQAPGAAAITGVPSPRATDPPSHLDGPPAVAVGQCPLVGPGPMHRRHLLLPARIPRPGLSEARCLLLFAAALAAAATLGCTGLVAYTGGLTPVWCFPGATFAP
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

full length protein

Tag Info

N-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Non-receptor serine/threonine protein kinase which is necessary for the maintenance of skeletal muscle structure and function. May play a role in myocyte differentiation and survival by regulating the integrity of the nuclear envelope and the expression of muscle-specific genes. May also phosphorylate PPP1R12A and inhibit the myosin phosphatase activity to regulate myosin phosphorylation. Also critical to the modulation of cardiac contractility and to the maintenance of proper cardiac conduction activity probably through the regulation of cellular calcium homeostasis. Phosphorylates PLN, a regulator of calcium pumps and may regulate sarcoplasmic reticulum calcium uptake in myocytes. May also phosphorylate FXYD1/PLM which is able to induce chloride currents. May also play a role in synaptic plasticity.

Gene References into Functions

Our results support the feasibility and safety of using antisense oligonucleotides for post-transcriptional silencing of DMPK in muscle and heart. PMID:27522499
Sense DMPK RNA foci clearly co-localize with MBNL1 and MBNL2 proteins and accumulate in myotonic dystrophy 1 tissues during development. PMID:26339785
The data of this study demonstrated that DMPK knockout mice present altered beta-agonist-induced responses and suggest that this is due, at least in part, to a reduced density of beta(1)-adrenergic receptors in cardiac plasma membranes. PMID:22190319
Cytosolic DMPK participates in remodeling of the actomyosin cytoskeleton in developing skeletal muscle cells. PMID:21295081
Muscleblind1, but not Dmpk or Six5, contributes to a complex phenotype of muscular and motivational deficits in mouse models of myotonic dystrophy PMID:20360842
a tail-anchored myotonic dystrophy protein kinase isoform induces perinuclear clustering of mitochondria, autophagy, and apoptosis PMID:19946639
Dmpk deficiency results in a sodium (Na) channel abnormality comprising frequent, long bursts of Na channel reopenings during sustained depolarization resulting in a plateau of non-inactivating late Na current. PMID:12454205
DMPK has a modulatory role in the control of intracellular Ca2+ concentration in mouse ventricular cardiomyocytes, loss of which contributes to cardiac dysfunction in myotonic dystrophy. PMID:12595579
role for DMPK in synaptic plasticity that could be relevant to the cognitive dysfunction associated with myotonic dystrophy PMID:12612014
DMPK isoforms have cell-type and location dependent substrate specificities with a role in organellar and cytoarchitectural dynamics PMID:12897125
Dmpk mRNA is expressed in a range of adult mouse tissues that including skeletal muscle, heart, smooth muscle, bone, testis, pituitary, brain, eye, skin, thymus,lung,intestinal epithelium, cartilage and liver. PMID:15336691
DMPK phosphorylates phospholamban and regulates calcium uptake in cardiomyocyte sarcoplasmic reticulum PMID:15598648
findings indicate that reduced DMPK expression may directly influence the onset of insulin-resistance in DM1 patients and point to dmpk as a new candidate gene for susceptibility to type 2-diabetes PMID:17987120
increased CUGBP1 protein levels are associated with DMPK-CUG RNA expression PMID:18272483
DM knockout macrophages infected with Mycobacterium tuberculosis in vitro were unable to stimulate pathogen-specific T cells. PMID:18591414
Data show that during cardiac myocyte maturation, DMPK migrates from perinuclear to cellular membrane localization. Manipulating DMPK levels in cultured cardiac and skeletal myocytes has revealed a key role for DMPK in myocyte differentiation. PMID:18729234
The cellular location of CUG RNA aggregates is an important variable that influences toxicity in myotonic dystrophy pathology. PMID:19092997
high-fat fed DMPK knockout mice had significantly increased body weights, hypertrophic adipocytes and whole-body insulin resistance compared with wild-type mice. PMID:19482024

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Subcellular Location

Sarcoplasmic reticulum membrane. Cell membrane. Note=Localizes to sarcoplasmic reticulum membranes of cardiomyocytes.; [Isoform 1]: Endoplasmic reticulum membrane; Single-pass type IV membrane protein; Cytoplasmic side. Nucleus outer membrane; Single-pass type IV membrane protein; Cytoplasmic side.; [Isoform 8]: Mitochondrion outer membrane; Single-pass type IV membrane protein.; [Isoform 5]: Cytoplasm, cytosol.

Protein Families

Protein kinase superfamily, AGC Ser/Thr protein kinase family, DMPK subfamily

Tissue Specificity

Expressed in all tissues tested, with a predominance in brain, skeletal muscle, heart, and other tissues containing smooth muscle. In the heart, expression is restricted to the cardiomyocytes in the ventricle and atrium.

Database Links

UNIGENE: Mm.6529

KEGG: mmu:13400

STRING: 10090.ENSMUSP00000032568

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