Recombinant Mouse Leukosialin (Spn)

Code: CSB-CF022590MO

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20μg100μg

US$5308

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Express system: in vitro E.coli expression system

Species: Mus musculus (Mouse)

Tag Info: N-terminal 10xHis-tagged

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Target Background

Product Details

Target Names

Spn

Uniprot NO.

P15702

Alternative Names

Spn; Leukosialin; B-cell differentiation antigen LP-3; Leukocyte sialoglycoprotein; Lymphocyte antigen 48; Ly-48; Sialophorin; CD antigen CD43

Species

Mus musculus (Mouse)

Source

in vitro E.coli expression system

Expression Region

20-395

Target Protein Sequence

DSLQRTTMLPSTPHITAPSTSEAQNASPSVSVGSGTVDSKETISPWGQTTIPVSLTPLETTELSSLETSAGASMSTPVPEPTASQEVSSKTSALLPEPSNVASDPPVTAANPVTDGPAANPVTDGTAASTSISKGTSAPPTTVTTSSNETSGPSVATTVSSKTSGPPVTTATGSLGPSSEMHGLPATTATSSVESSSVARGTSVSSRKTSTTSTQDPITTRSPSQESSGMLLVPMLIALVVVLALVALLLLWRQRQKRRTGALTLSGGGKRNGVVDAWAGPARVPDEEATTTSGAGGNKGSEVLETEGSGQRPTLTTFFSRRKSRQGSLVLEELKPGSGPNLKGEEEPLVGSEDEAVETPTSDGPQAKDEAAPQSL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN). Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment. Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4(+) T-cells and to a lesser extent by CD8(+) T-cells. Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR. Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E-selectin. Acts as a T-cell counter-receptor for SIGLEC1.; Protects cells from apoptotic signals, promoting cell survival.

Gene References into Functions

glycosylation status of CD43 protein on leukemia cells is associated with sensitivity to CTL-mediated cytolysis PMID:27011118
findings demonstrate that CD43 is a major E-selectin ligand in Th17 cells that functions independent of P-selectin glycoprotein ligand-1 PMID:26700769
CD43 may be an important mediator of macrophage lipid homeostasis, thereby affecting macrophage foam cell formation and ultimately atherosclerotic plaque development. PMID:25524774
We have identified a robust physiologic role for CD43 in a relevant animal model and established an important in vivo function for CD43-dependent regulation of IFN-gamma production. PMID:23585675
Results show that while CD43 binding to ezrin-radixin-moesin proteins is crucial for S76 phosphorylation, CD43 movement and regulation of T-cell migration can occur through an ERM-independent, phosphorylation-dependent mechanism. PMID:21289089
study found that the M. tuberculosis Cpn60.2 molecular chaperone is the major mycobacterial surface ligand that interacts with CD43 to stabilize the interaction between M. tuberculosis and the macrophage PMID:20633027
Transient resistance to B16F10 melanoma growth and metastasis in CD43-/- mice. PMID:11828253
CD43 engagement and signaling are involved in the costimulatory and antiapoptotic effects of enzymatically inactive mutant trypanosomal trans-sialidase when it binds highly sialylated mucin CD43 on the T cell surface. PMID:11994475
Cd43 displays dynamic roles in T cell immunity: a positive regulatory role in costimulation and trafficking of T cells to the central nervous system, and a negative regulatory role in the down-modulation of an immune response. PMID:12055210
Delayed onset of experimental autoimmune encephalomyelitis in CD43-deficient immunized mice and in recipients of adoptively transferred CD43-/- cells is characterized by significant reduction of T cell trafficking to the brain and spinal cord. PMID:14662853
Exclusion of the CD43 intracellular region from the immunological synapse is required for CD43 regulation of interleukin-2 production. PMID:15117976
Two functionally distinct populations of small intestinal intraepithelial lymphocytes (IELs) are described that differ according to the expression of a CD43 glycoform. PMID:15528368
CD43 is involved in the binding, uptake, and subsequent growth of Mycobacterium tuberculosis in murine macrophages and in vivo PMID:16034122
That CD43 costimulation was responsible for elevated cytokine/chemokine activity was confirmed at the transcriptional level by real-time PCR for IFN-gamma and CCL5, and by ELISA for IFN-gamma. PMID:16246302
CD43, when modified by a specific set of glycosyltranferases, can function as an E-selectin ligand and therefore potentially mediate activated T cell migration PMID:16339541
These results characterize an important role for CD43 during the activation and expansion of autoreactive MOG-specific CD8+ T cells. PMID:16890924
Recruitment of neutrophils and activated T cells in in vivo models is not influenced by CD43 expression. PMID:17056577
CD43 functions as a signaling molecule, sensing extracellular cues and transducing intracellular signals that modulate T-cell function PMID:17638845
results demonstrate that CD43 regulates Th cell differentiation in vitro and in vivo PMID:18490738
The authors propose that CD43 is involved in the inflammatory response to M. tuberculosis and, through the induction of pro-inflammatory mediators, can regulate apoptosis to control intracellular growth of the bacterium. PMID:18637079
The loss of both PSGL-1 and CD43 led to a further increase in T cell adhesion and proliferation PMID:19890058

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Subcellular Location

Membrane; Single-pass type I membrane protein. Cell projection, microvillus. Cell projection, uropodium.; [CD43 cytoplasmic tail]: Nucleus. Nucleus, PML body.

Tissue Specificity

Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.

Database Links

UNIGENE: Mm.283714

KEGG: mmu:20737

STRING: 10090.ENSMUSP00000049534

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