HEK293T/Human ENPP3 Stable Cell Line

CSB-SC007681HU

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Size:

1 vial contains approximately 5x10⁶ cells in 1 ml

US$5000

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Product Details
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Target Background

Product Details

Growth Properties

Adherent

Selection Marker

Puromycin

Culture Medium

DMEM + 10% FBS + 2μg/mL Puromycin

Tag Info

Tag-Free

Buffer

35% FBS + 55% DMEM + 10% DMSO

Storage

Frozen in liquid nitrogen or stored at -80°C

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Protocols

Images

Untransfected HEK293T cells (green line) and transfected Human ENPP3 HEK293T Stable cells (red line) were stained with anti-ENPP3 antibody (2µg/1*10⁶cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

Description

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Target Background

Function

Hydrolase that metabolizes extracellular nucleotides, including ATP, GTP, UTP and CTP. Limits mast cell and basophil responses during inflammation and during the chronic phases of allergic responses by eliminating the extracellular ATP that functions as signaling molecule and activates basophils and mast cells and induces the release of inflammatory cytokines. Metabolizes extracellular ATP in the lumen of the small intestine, and thereby prevents ATP-induced apoptosis of intestinal plasmacytoid dendritic cells. Has also alkaline phosphodiesterase activity.

Gene References into Functions

We showed the ability of the immunological marker CD203c to predict the clinical efficacy of sublingual immunotherapy on rhinitis subjects allergic to Parietaria. PMID:29702283
Basophil CD203c surface expression reliably discriminated cystic fibrosis with allergic bronchopulmonary aspergillosis from cystic fibrosis with Aspergillus colonization and cystic fibrosis over time. PMID:26585435
Expression of CD203c on basophils as a marker of immunoglobulin E-mediated (L)-asparaginase allergy. PMID:23581640
Anaphylactic transfusion reaction in homozygous haptoglobin deficiency detected by CD203c expression on basophils. PMID:24497482
ENPP3 is a regulator of N-acetylglucosaminyltransferase GnT-IX (GnT-Vb) PMID:23960081
The early signaling requirements for the CD11b/CD203c compartment expression and CD63 degranulation provide support for the hypothesis that CD11b and CD203c reside in a similar compartment. PMID:22722613
Subjects with nut allergy show an increase of basophil CD203c levels at baseline and following rapid ex vivo stimulation with nut allergen PMID:20975283
Asthma exacerbation was accompanied by increased expression of CD203c on basophils that decreased significantly during remission PMID:20159259
Influence of hyperosmotic conditions on basophil CD203c upregulation in patients with food-dependent exercise-induced anaphylaxis. PMID:20047266
Data show that low and high dilutions of histamine inhibit CD203c up-regulation in anti-IgE stimulated basophils. PMID:19418203
E-NPP3 is associated with carcinogenesis of human colon cancer and that serum E-NPP3 might be a tumor marker of colon carcinoma PMID:14533006
E-NPP3 is involved in the infiltration of neoplastic bile duct carcinoma PMID:15072822
Our data demonstrate that leptin promotes platelet activation, provides a mechanistic basis for the prothrombotic effect of this hormone, and identifies a potentially novel therapeutic avenue to limit obesity-associated cardiovascular disease. PMID:15886225
In conclusion, using well-defined experimental conditions, the measurement of CD203c up-regulation on basophils in response to specific allergens is as reliable as CD63-BAT for the in vitro diagnosis of patients with IgE-mediated allergy. PMID:17275019