WDR5 Recombinant Monoclonal Antibody

Code: CSB-RA620753A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, IHC, FC

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Target Background

Product Details

Uniprot NO.

P61964

Target Names

WDR5

Alternative Names

WD repeat-containing protein 5 (BMP2-induced 3-kb gene protein), WDR5, BIG3

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human WDR5

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

18D11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Immunohistochemistry (IHC) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

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Images

IHC image of CSB-RA620753A0HU diluted at 1:50 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.79% DAB.
Overlay Peak curve showing Hela cells stained with CSB-RA620753A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

WDR5 serves as a critical scaffolding component within the MLL/SET histone methyltransferase complexes, where it facilitates the methylation of histone H3 at lysine 4, a modification strongly associated with active gene transcription. This WD40 repeat-containing protein has emerged as a significant focus in epigenetics research, particularly given its roles in embryonic development, stem cell self-renewal, and its documented involvement in various malignancies where it can act as a transcriptional coactivator for oncogenic programs.

This recombinant monoclonal antibody, clone 18D11, offers researchers the reproducibility advantages inherent to recombinant technology. Because the antibody sequence is defined and production occurs through controlled recombinant expression in rabbit host cells, you can expect consistent performance across experiments and between lot numbers, eliminating a common source of variability in long-term studies. Affinity chromatography purification ensures high purity suitable for demanding applications.

Validation testing demonstrates reliable performance across multiple experimental platforms. Immunohistochemistry studies using paraffin-embedded human testis tissue at dilutions between 1:50 and 1:200 show specific staining following citrate buffer antigen retrieval. Flow cytometry analysis in HeLa cells confirms the antibody's utility for intracellular detection of WDR5, with clear separation between specific signal and isotype control when cells are fixed and permeabilized. These validated applications provide flexibility for researchers investigating WDR5 in both tissue context and single-cell analysis workflows.

For laboratories exploring chromatin regulation, transcriptional control mechanisms, or the epigenetic landscape of disease states, this antibody provides a dependable tool for detecting WDR5 in human samples across immunohistochemical and flow cytometric applications.

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Target Background

Function(From Uniprot)

Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. May regulate osteoblasts differentiation. In association with RBBP5 and ASH2L, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B.

Gene References into Functions

WDR5 was identified as a substrate of SETD6 and it was revealed that the methylation of specific lysines (K207/K325) of WDR5 was critical for maintaining global histone H3K4me3 levels and promoting breast cancer cell proliferation and migration PMID:30226578
WDR5 is aberrantly overexpressed in head neck squamouos cell carcinoma and associates with aggressiveness and unfavorable prognosis, thus representing a novel diagnostic and prognostic biomarker for the cancer. PMID:29569374
This is the first demonstration of the mechanism of E2/ERalpha signalling activation via the BIG3-PKA-PP1Calpha tri-complex in breast cancer cells. PMID:28555617
Data found that the expression of WDR5 increased in a majority of the human gastric cancer tissues. Its knockdown significantly inhibited the ability of cell proliferation, reversed cell cycle arrest as well as cell's tumorigenicity. Data reveal that WDR5 may have oncogenic effect and WDR5-mediated H3K4 methylation plays an important role in gastric cancer. PMID:29125890
findings reveal a nongenomic function for WDR5 in regulating H3K4 methylation induced by 3D environments, physical properties of the nucleus, cell polarity, and cell migratory capacity. PMID:29987046
HCMV infection increases protein levels of WD repeat-containing protein 5 (WDR5) during infection, overexpression of WDR5 enhances viral replication, and knockdown of WDR5 dramatically attenuates viral replication. The results indicate that WDR5 promotes the nuclear egress of viral capsids, the depletion of WDR5 resulting in a significant decrease in production of infectious virions. PMID:29437978
our findings indicate that HOXD-AS1 promotes proliferation, castration resistance, and chemo-resistance in prostate cancer by recruiting WDR5. PMID:28487115
Functional analysis implicates TNRC6A, NAT10, MED14, and WDR5 in RNA-mediated transcriptional activation. PMID:28813667
The decrease in WDR5 expression was particularly evident in HD-hiPSCs compared to hESCs and control-hiPSCs from healthy subjects PMID:28476540
we demonstrate for the first time that targeted expression of miR-31-5p using a nonviral minicircle vector can serve as a novel approach for tumor miRNA therapy. Moreover, WDR5 may be a promising therapeutic target for NPC treatment. PMID:28042945
WDR5 may have oncogenic effect and WDR5-mediated H3K4 methylation plays an important role in leukemogenesis PMID:27192115
WDR5 functions to sustain proper execution of DNA replication in pancreatic ductal adenocarcinoma cells, as previously suggested by replication stress studies involving MLL1, and c-Myc, also found to interact with WDR5. PMID:27320920
This report documents a novel lncRNA, GClnc1, which may act as a scaffold to recruit the WDR5 and KAT2A complex and modify the transcription of target genes. This study reveals that GClnc1 is an oncogenic lncRNA in human gastric cancer. PMID:27147598
different cancer mutations in MLL1 lead to a loss or increase in activity, illustrating the complex and tumor-specific role of MLL1 in carcinogenesis. PMID:28182322
WDR5 shows a direct binding to the ZNF407 promoter. PMID:28300833
Taken together, these data suggest that WDR5 is directly involved in p53 signaling pathway. Our studies provide a new insight into WDR5 functions in A549 cells. PMID:28412363
Targeted Disruption of the Interaction between WD-40 Repeat Protein 5 (WDR5) and Mixed Lineage Leukemia (MLL)/SET1 Family Proteins Specifically Inhibits MLL1 and SETd1A Methyltransferase Complexes. PMID:27563068
WDR5 over-expression is associated with poor breast cancer clinical outcome. PMID:26355959
Results identify WDR5 as a key cofactor for N-Myc-regulated transcriptional activation and tumorigenesis and as a novel therapeutic target for MYCN-amplified neuroblastomas. PMID:26471359
BIG3 may block the KPNAs (KPNA1, KPNA5, and KPNA6) binding region(s) of PHB2. PMID:26052702
solution structures of the MLL3 core complex assembled with and without WDR5 by small angle x-ray scattering show similar overall topologies PMID:26324722
Target gene recognition by MYC depends on its interaction with WDR5. This interaction could create an avidity-based chromatin recognition mechanism allowing MYC to select its target genes in response to both genetic and epigenetic determinants. Review. PMID:26383167
we have discovered that WDR5 plays an important role in bladder cancer suggesting that WDR5 is a potential biomarker and a promising target in the treatment of bladder cancer. PMID:25656485
Wdr5-MLL interaction in C/EBPA N-terminal leukemia is a promising pharmacological target. PMID:26167872
findings prove that the TSP-1/CD47/SIRP-alpha signal axis is important to the evolution of tumor cells in the microenvironment of immunotherapy and identify thrombospondin-1 as a key signal PMID:25666610
results identify WDR5 as a critical epigenomic integrator of histone phosphorylation and methylation and as a major driver of androgen-dependent prostate cancer cell proliferation PMID:24793694
BIG3(WRD5)-PHB2 interaction is critical for the tamoxifen resistance of breast cancer cells; its targeting reverses the resistance. PMID:24051437
Data indicate that MLL1 methylates Ash2L in the absence of histone H3, but only when assembled within a complex with WDR5 and RbBP5. PMID:24235145
data are consistent with a model in which WDR5 binds the gamma-globin promoter in a PRMT5-dependent manner. PMID:22689669
A small molecule antagonist binds to WDR5 in the peptide-binding pocket which inhibits the catalytic activity of mixed-lineage leukemia protein. PMID:22989411
we defined a role for Trithorax proteins WDR5 and MLL2 in activating the differentiation gene program in epidermal progenitor cells PMID:22829784
WDR5-Win motif interaction is important for the assembly of the MLL1 core complex in vivo. PMID:22665483
findings show that WDR5 is a direct target of SRY; the interaction of WDR5 and SRY activates Sox9 expression; results suggest that, in conjunction with SRY, WDR5 plays an important role in sex determination PMID:22523547
WDR5 as a critical substrate of CUL4B in regulating neuronal gene expression. PMID:21816345
crystal structure of WDR5 in ternary complex with RbBP5 and MLL1 PMID:21220120
the change of folding free energy by mutations mainly corresponds to the deletion of hydrogen bonds. PMID:20939513
NSL is composed of nine subunits. Two of its subunits, WD repeat domain 5 (WDR5) and host cell factor 1 (HCF1), are shared with members of the MLL/SET family of histone H3 lysine 4 (H3K4) methyltransferase complexes. PMID:20018852
Results show that Depletion of CHD8 enhances HOXA2 expression and a loss of the WDR5/Ash2L/RbBP5 subcomplex. PMID:20085832
WDR5 is essential in assembling a virus-induced VISA-associated complex and plays an important role in virus-triggered induction of type I interferons. PMID:20080758
The WDR5 is required for binding of the methyltransferase complex to the K4-dimethylated H3 tail as well as for global H3 K4 trimethylation and HOX gene activation in human cells. PMID:15960974
Wdr5 has a funcitonal role in endocondral bone formation PMID:16340128
the high-resolution X-ray structures of WDR5 in the unliganded form and complexed with histone H3 peptides having unmodified and mono-, di- and trimethylated K4 PMID:16829959
the crystal structure of WDR5 bound to a histone H3 PMID:16829960
WDR5 mediates interactions of the MLL1 catalytic unit both with the common structural platform and with the histone substrate. PMID:16878130
involvement of WDR5 in binding and presenting histone H3K4 for further methylation PMID:16946699
WDR5's recognition of arginine 3765 of MLL1 is essential for the assembly and enzymatic activity of the MLL1 core complex in vitro PMID:18829457
WDR5 recognizes Arg-3765 of MLL1, which is essential for the assembly and enzymatic activity of the MLL1 core complex PMID:18829459
analysis of interactions between WDR5, the catalytic subunit, MLL, and the substrate, histone H3, of the MLL complex PMID:18840606
The identified components revealed factors involved in histone methylation and cell cycle control and include Ash2L, RbBP5, WDR5, HCF-1, DBC-1, and EMSY. PMID:19131338
Activation of an estrogen/estrogen receptor signaling by BIG3 through its inhibitory effect on nuclear transport of PHB2/REA in breast cancer is reported. PMID:19496786