UCHL1 Recombinant Monoclonal Antibody

Code: CSB-RA261594A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human, Mouse, Rat

Application: ELISA, WB, IHC

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Target Background

Product Details

Uniprot NO.

P09936

Target Names

UCHL1

Alternative Names

Epididymis luminal protein 117 antibody; Epididymis secretory protein Li 53 antibody; HEL 117 antibody; HEL S 53 antibody; NDGOA antibody; Neuron cytoplasmic protein 9.5 antibody; OTTHUMP00000218137 antibody; OTTHUMP00000218139 antibody; OTTHUMP00000218140 antibody; OTTHUMP00000218141 antibody; Park 5 antibody; PARK5 antibody; PGP 9.5 antibody; PGP9.5 antibody; PGP95 antibody; Protein gene product 9.5 antibody; Ubiquitin C terminal esterase L1 antibody; Ubiquitin C terminal hydrolase antibody; Ubiquitin C terminal hydrolase L1 antibody; Ubiquitin carboxyl terminal esterase L1 antibody; Ubiquitin carboxyl terminal hydrolase isozyme L1 antibody; Ubiquitin carboxyl-terminal hydrolase isozyme L1 antibody; Ubiquitin thioesterase L1 antibody; Ubiquitin thiolesterase antibody; Ubiquitin thiolesterase L1 antibody; UCH-L1 antibody; UCHL1 antibody; UCHL1_HUMAN antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human UCHL1

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

13A5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: HEK293T whole cell lysate(20µg), U-251MG whole cell lysate(20µg),A549 whole cell lysate(20µg),SH-SY5Y whole cell lysate(20µg),NTERA-2 whole cell lysate(20µg), Mouse brain tissue lysate(20µg), Rat brain tissue lysate(20µg)
All lanes: PGP9.5 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 25 kDa
Observed band size: 26 kDa
Exposure time：50s
IHC image of CSB-RA261594A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Description

UCHL1, also widely recognized as PGP9.5, serves as a critical deubiquitinating enzyme predominantly expressed in neurons and neuroendocrine cells. This protein plays an essential role in maintaining ubiquitin homeostasis by hydrolyzing ubiquitin from degraded proteins, enabling ubiquitin recycling within the proteasome pathway. Beyond its enzymatic function, UCHL1 has emerged as a significant biomarker in neurodegenerative disease research, with mutations linked to familial Parkinson's disease, and its expression patterns provide valuable insights into neuronal integrity and neuroendocrine tumor characterization.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human UCHL1, offers the reproducibility and consistency that demanding research applications require. As a sequence-defined recombinant clone, it eliminates the lot-to-lot variability inherent in traditional hybridoma-derived antibodies, ensuring your experimental results remain comparable across extended studies.

Validation testing demonstrates robust performance across multiple experimental platforms. Western blot analysis confirms reliable detection in human cell lines including HEK293T, U-251MG glioblastoma, A549 lung carcinoma, SH-SY5Y neuroblastoma, and NTERA-2 embryonal carcinoma cells, with an observed band at approximately 26 kDa. The observed ~26 kDa band is likely due to post-translational modifications (e.g., phosphorylation or glycosylation) or inherent SDS-PAGE migration discrepancies causing a slight upward shift from the predicted 25 kDa. Cross-species reactivity extends to mouse and rat brain tissue lysates, supporting translational neuroscience workflows. Immunohistochemistry validation in paraffin-embedded human kidney tissue demonstrates suitability for tissue-based studies examining UCHL1 distribution patterns.

Whether investigating neurodegenerative mechanisms, characterizing neuroendocrine tumors, or exploring ubiquitin pathway dynamics, this antibody provides a dependable tool for researchers requiring consistent, validated performance across Western blot, immunohistochemistry, and ELISA applications.

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Target Background

Function(From Uniprot)

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.

Gene References into Functions

The proteins UCH-L1 and alpha-internexin could be independent prognostic biomarkers of pancreatic neuroendocrine tumors. PMID:28526880
We have also demonstrated that UCHL1 S-nitrosylation provides seeding for faster aggregation of a-synuclein. Finally, the in vitro nitrosylation of UCHL1 was corroborated with rotenone induced mouse model of PD PMID:28300150
In newborns undergoing cardiac surgery, ubiquitin C-terminal hydrolase 1 showed a significant rise at 0 hours in the deep hypothermic circulatory arrest group compared to baseline. Levels returned to baseline at 12 hours. There was an early rise in UCHL1 at 0 hours in the cardiopulmonary bypass group. PMID:29945509
Overexpression of UCHL1 genes promoted apoptosis in cells treated with VER+ADM. UCHL1 knockdown using siRNA weakened the effect of ADM+VER, indicating that ADM+VER promotes HCC cell apoptosis and that UCHL1 genes participate in VER-mediated promotion in tumor cell apoptosis. PMID:29627846
Cholangiocarcinoma patients who had high methylation of HTATIP2 and low methylation of UCHL1 showed longer overall survival than those with low HTATIP2 methylation and high UCHL1 methylation. PMID:29359783
High UCHL1 expression is associated with posterior longitudinal ligament ossification. PMID:29782494
Prx II exhibited more effective molecular chaperone activity than Prx I when UCH-L1 was the client. Prx II interacted with UCH-L1 through its C-terminal region to protect UCH-L1 from thermal or oxidative inactivation PMID:29339092
Data report that the expression of UCH-L1 is significantly higher in breast cancer cells with higher invasive ability. Also, the overexpression of UCH-L1 led to activation of Akt and phosphorylation. Thus, these findings demonstrated that UCH-L1 promotes invasion of breast cancer cells. PMID:28636190
The authors observed higher serum levels of GFAP and UCH-L1 in brain-injured children compared with controls and also demonstrated a step-wise increase of biomarker concentrations over the continuum of severity from mild to severe traumatic brain injury. Serum UCH-L1 and GFAP concentrations also strongly predicted poor outcome. PMID:27319802
UCHL1 inhibits autophagy and is depending on its de-ubiquitinating activity. PMID:29462615
Uchl1 concentrations in the blood plasma of boys with cryptorchidism, may reflect the heat-induced apoptosis of germ cells PMID:29401475
Overexpression of UCHL1 is associated with thermal injury. PMID:28193576
UCHL1 localizes to TTAGGG repeats at telomeres and interstitial telomeric sequences. A weak or transient interaction between UCHL1 and the shelterin complex was confirmed by immunoprecipitation and proximity ligation assays. PMID:29126443
novel biomarker for identifying malignant potential of primary well-differentiated and moderately differentiated pancreatic neuroendocrine tumors PMID:29150024
detection of significantly higher levels of UCH-L1 in patients with ischemic stroke and intracranial hemorrhage compared to patients with metabolic disorder induced impaired consciousness and healthy volunteers PMID:28651886
This study also showed that UCH-L1 promotes angiogenesis of HUVECs, as well as invasion in cancer cells, by up-regulating ROS by deubiquitination of NOX4, suggesting that UCH-L1 plays a key role in angiogenesis of HUVECS by regulating ROS levels by deubiquitination of NOX4. PMID:29128359
Familial mutations and post-translational modifications of UCH-L1 in Parkinson's disease have been summarized. (Review) PMID:26899237
Aberrant expression of UCHL1 in pediatric high-grade gliomas may promote cell invasion, transformation, and self-renewal properties, at least in part, by modulating Wnt/Beta catenin activity PMID:28472177
Suppression of MITF activity by UCHL1 via protein degradation might aid in the development of new therapeutic approaches for melanoma or dyspigmentation disorders. PMID:28392346
Overexpression of UCH-L1 in MCF7 cells up-regulated MDR1, CD147, MMP2, and MMP9, which conferred multidrug resistance and promoted migration/invasion. PMID:26293643
Folding of a 52-Knotted Protein PMID:28002735
This study demonstrated that UCH-L1 was detectible within 1 hour of injury and rose rapidly and peaked at 8 hours after injury and declined rapidly over 48 hours. PMID:27018834
This study establishes the importance of UCHL1 in neurodegeneration, provides new mechanistic insight about ubiquitin processing, and underlines the complexity of the different roles of UCHL1 PMID:28007905
TGF-beta1 can promote PGP9.5 expression in Cancer associated fibroblasts to facilitate the growth of cancer cells. PMID:27840994
PGP9.5 is also expressed in benign fibroblastic lesions PMID:27914685
cytoplasmic accumulation of P53 was strongly associated with the unmethylated UCHL1 profile (P = 0.006), supporting the relationship between these two proteins in sporadic colorectal cancer PMID:26314856
indicated that the LDT is an accurate, robust and automated assay, which adequately and reliably identifies patients presenting with small fiber neuropathy, and therefore has potential for use in large scale clinical studies PMID:27215701
No difference in cord blood concentration found between hypoxic-ischemic encephalopathy neonates and controls PMID:26135781
UCHL1 has a role in Alzheimer Disease [review] PMID:26881020
UCHL1 expression is highly upregulated upon hepatic stellate cell (HSC) activation and is involved in the regulation of HSC proliferation PMID:26264933
UCH-L1 is a promising prognostic biomarker for GCAs and might play an important role in the carcinogenesis of gastric cancer. PMID:26823707
Folloing aneurysmal subarachnoid hemorrhage, eleevated UCH-L1 levels during the five-day follow-up were associated with unfavorable neurological outcome. PMID:26810533
UCH-L1 plays a crucial role in modulating the degradation of EGFR and promoting malignant properties in multi-drug resistant breast cancer PMID:26722437
High UCHL1 expression is associated with multiple myeloma. PMID:26513019
The neuronal marker UCH-L1 is induced in, and specifically augments the oncogene-induced transformation of, germinal center B cells. PMID:26702068
The promoter methylation degree of FLNC, THBS1, UCHL1, and DLEC1 in serum could tell the existence of GC and only UCHL1 in the serum was also associated with poor prognosis of GC. PMID:26550574
The results of this study suggest the role for UCHL1 in promoting Corticospinal motor neurons health and stability. PMID:25596590
UCH-L1 is significantly associated with outcome, but it does not add predictive power to commonly used prognostic variables in a population of patients with TBI of varying severities. PMID:26547005
Serum UCH-l1 levels serve as a novel biomarker for neuronal damage from white matter lesions. PMID:26232084
High Ubiquitin C-terminal hydrolase-L1 increases cancer cell invasion by modulating hydrogen peroxide generated via NADPH oxidase 4. PMID:25915537
These findings demonstrated that UCHL1 promoted cell proliferation, migration, and invasion depending on its de-ubiquitinase activity by activating Akt and Erk1/2 pathways PMID:26018507
These results suggest that ubiquitin C-terminal hydrolase and alphaII-spectrin breakdown product 145 kDa may be useful in assessing outcome after pediatric traumatic brain injury. PMID:22022780
Pathogenetic tau truncation may contribute to synaptic deterioration in Alzheimer disease by aberrant recruitment of Parkin and UCHL-1 to mitochondria. PMID:25687137
UCHL1 promotes metastases as a deubiquitinating enzyme for HIF-1alpha. PMID:25615526
Meta-analysis suggesting that UCHL1 S18Y polymorphism is moderately associated with susceptibility to Parkinson's disease PMID:25370916
Potential of UCHL1 as biomarker for destruction of pancreatic beta cells. PMID:25638021
Data identified a variant of UCH-L1 lacking N-terminal 11 amino acids designated as NT-UCH-L1 and was found to have a protective role in the Parkinson's disease model in vitro and in vivo. PMID:24959670
UCHL1 may delay Alzheimer's progression by regulating APP degradation. PMID:25466238
miR-922 increasing the levels of phosphorylated tau by regulating UCHL1 levels contributed to the pathogenesis of Alzheimer diseases. PMID:24950120
Skin PGP 9.5 expression, a neuronal marker, was significantly lower in patients with familial transthyretin amyloid neuropathy compared to controls. PMID:25973863

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Subcellular Location

Cytoplasm. Endoplasmic reticulum membrane; Lipid-anchor. Note=About 30% of total UCHL1 is associated with membranes in brain.

Protein Families

Peptidase C12 family

Tissue Specificity

Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and A

Database Links

HGNC: 12513

UNIGENE: Hs.518731

KEGG: hsa:7345

STRING: 9606.ENSP00000284440

OMIM: 191342

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