UBB/UBC Recombinant Monoclonal Antibody

Ubiquitin, encoded by the UBB and UBC genes, serves as the fundamental molecular tag in the ubiquitin-proteasome system, one of the most critical regulatory pathways in eukaryotic cells. This small protein governs protein turnover, cell cycle progression, DNA repair, and signal transduction through its covalent attachment to target substrates. Dysregulation of ubiquitin-mediated processes has been implicated in neurodegenerative diseases, cancer progression, and immune dysfunction, making ubiquitin detection essential for researchers investigating proteostasis and cellular stress responses.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human UBB/UBC protein, offers the reproducibility and consistency that demanding experimental workflows require. As a sequence-defined reagent produced from a single rabbit clone, it eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The affinity-purified IgG format ensures minimal background while maintaining strong target recognition.

Validation studies demonstrate reliable performance across multiple detection platforms. In immunohistochemistry, the antibody produces clear staining in paraffin-embedded human lung cancer tissue at dilutions of 1:50 to 1:200, with optimal results observed at 1:100 using citrate buffer antigen retrieval. Immunofluorescence applications reveal distinct cellular localization patterns in HeLa cells at 1:50 dilution, enabling visualization of ubiquitin distribution within subcellular compartments. The antibody is also validated for ELISA-based quantitative detection.

Whether you are investigating proteasomal degradation pathways, characterizing ubiquitin conjugates in disease models, or examining protein quality control mechanisms, this antibody provides a dependable tool for ubiquitin detection in human samples across tissue-based and cell-based experimental systems.