TRIM24 Recombinant Monoclonal Antibody

Code: CSB-RA133402A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, WB, IHC, FC

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Target Background

Product Details

Uniprot NO.

O15164

Target Names

TRIM24

Alternative Names

E3 ubiquitin protein ligase TRIM24 antibody; E3 ubiquitin-protein ligase Trim24 antibody; hTIF1 antibody; PTC6 antibody; RING finger protein 82 antibody; RNF82 antibody; TF1A antibody; TIF1 alpha antibody; TIF1 antibody; TIF1-alpha antibody; TIF1A antibody; TIF1A_HUMAN antibody; TIF1ALPHA antibody; Transcription intermediary factor 1-alpha antibody; Transcriptional intermediary factor 1 alpha antibody; Transcriptional intermediary factor 1 antibody; Trim24 antibody; Tripartite motif containing 24 antibody; Tripartite motif-containing protein 24 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human TRIM24 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

3A2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: MCF-7 whole cell lysate(30µg)
All lanes: TRIM24 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 117 kDa
Observed band size: 130 kDa
Exposure time：2min
IHC image of CSB-RA133402A0HU diluted at 1:100 and staining in paraffin-embedded human thyroid tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA133402A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Description

TRIM24, also known as transcription intermediary factor 1-alpha (TIF1α), functions as an E3 ubiquitin-protein ligase that plays critical roles in transcriptional regulation and chromatin remodeling. This tripartite motif-containing protein has emerged as a significant focus in signal transduction research, particularly for its involvement in nuclear receptor signaling pathways and its documented associations with various disease states where transcriptional control becomes dysregulated.

This recombinant monoclonal antibody, clone 3A2, offers the reproducibility and consistency that demanding experimental workflows require. Generated against a synthetic peptide derived from human TRIM24, the antibody is produced using recombinant technology, ensuring sequence-defined specificity and eliminating the lot-to-lot variability that can compromise longitudinal studies. The rabbit IgG format, purified by affinity chromatography, delivers reliable performance across multiple detection platforms.

Validation studies demonstrate robust performance in Western blot analysis, where the antibody detects TRIM24 in MCF-7 whole cell lysates at dilutions ranging from 1:500 to 1:5000. The observed band at approximately 130 kDa runs slightly higher than the predicted 117 kDa molecular weight, a shift commonly attributed to post-translational modifications such as glycosylation or phosphorylation events characteristic of this protein. Immunohistochemistry applications have been validated using paraffin-embedded human thyroid tissue, while flow cytometry performance has been confirmed in SH-SY5Y neuroblastoma cells, providing researchers with flexibility across tissue and cell-based experimental systems.

Whether investigating TRIM24's role in transcriptional regulation, exploring its E3 ligase activity, or examining its contributions to signal transduction pathways, this antibody provides a dependable tool for advancing your research objectives.

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Target Background

Function(From Uniprot)

Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. Promotes ubiquitination and proteasomal degradation of p53/TP53. Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes.

Gene References into Functions

these results suggested that TRIM24 has an important role in the growth of Nasopharyngeal carcinoma (NPC). Additionally, silenced TRIM24 may lead to inhibited cell proliferation and induced cell apoptosis in NPC cells. PMID:29749443
TRIM24 is upregulated in HNSCC and promotes HNSCC cell growth and invasion through modulation of cell cycle, glucose metabolism, and GLUT3, making TRIM24 a potential oncoprotein in HNSCC. PMID:29862279
The findings identify TRIM24 as an oncogenic transcriptional co-activator in epidermal growth factor receptor-driven glioblastoma and also demonstrate a previously unknown signal relay by which H3K23ac/TRIM24 mediates epidermal growth factor receptor stimulation of STAT3 activation, thereby enhancing the oncogenic activity of the epidermal growth factor receptor/STAT3 pathway in human cancers. PMID:29129908
Results suggest that TRIM24 is a novel coactivator of the CAR that is involved in cell- and/or promoter- selective transactivation. PMID:29101097
TRIM24 expression was increased in human colorectal cancer and might be a novel prognostic biomarker. PMID:28916426
Study showed that TRIM24 was upregulated during gastric carcinogenesis and demonstrated that TRIM24 was a functional target gene of miR-511, and miR-511 inactivated PI3K/AKT and Wnt/beta-catenin pathways by suppressing TRIM24. PMID:28114950
we identified altered glucose metabolism in the progression of head and neck squamous cell carcinoma and showed that it could be partially attributed to the novel link between GLUT4 and TRIM24 PMID:28061796
This study concluded that reduced TRIM24 protein is associated with poor survival in esophageal squamous cell cancer (ESCC) patients, suggesting TRIM24 protein is a potential prognostic biomarker for ESCC. PMID:27689360
Data suggest that, in cardiomyocytes, TRIM32 attenuates activation of SRF signaling and hypertrophy due to dysbindin; TRIM24 promotes these effects. TRIM32 promotes dysbindin degradation; TRIM24 protects dysbindin from degradation. (TRIM = tripartite motif-containing protein; SRF = serum response factor) PMID:28465353
hypothesis of "synergistic modification induced recognition" is then proposed to link histone modification and TRIM24 binding PMID:27079666
Report provides evidence for an oncogenic role for TRIM24 as a transcriptional activator and mediator of hormone-refractory prostate cancer cell growth in SPOP mutant and castration-resistant prostate cells. PMID:27238081
TRIM24 expression is positively correlated with Acetylated H3 lysine 23 levels, and high levels of both TRIM24 and Acetylated H3 lysine 23 predict shorter overall survival of breast cancer patients. PMID:27638829
TRIM24 regulate resistance to Gefitinib via Akt pathway in non-small cell lung cancer cells. PMID:26805734
TRIM24 is overexpressed in human bladder cancer and facilitates bladder cancer growth and invasion, possibly through NF-kappaB and AKT signaling pathways. PMID:25846736
functions as an oncogene in colorectal carcinogenesis PMID:25700357
A role for TRIM24 in breast tumorigenesis through reprogramming of glucose metabolism in human mammary epithelial cells, further supporting TRIM24 as a viable therapeutic target in breast cancer. PMID:25065590
our study shows that TRIM24 is overexpressed in human gastric cancer and accelerates cell growth as well as induce chemoresistance PMID:25724180
Our results suggest that TRIM24 might serve as a potential prognostic marker and therapeutic target for the management of malignant gliomas. PMID:24469053
Study shows that TRIM24 is destabilized by ATM-mediated phosphorylation of TRIM24S768 in response to DNA damage, which disrupts TRIM24-p53 interactions and promotes the degradation of TRIM24. PMID:24820418
Overexpression of TRIM24 is associated with the onset and progress of human hepatocellular carcinoma. PMID:24409330
Upon TRIM24 silencing, the proliferation of HNSCC cells was notably inhibited due to the induction of apoptosis. PMID:23717505
TRIM24 plays an important role in NSCLC progression PMID:22666376
These results suggest that E4-ORF3 targets proteins for relocalization through a loosely homologous sequence dependent on accessibility. PMID:22123502
overexpression of the TRIM24/TIF-1alpha gene in breast cancer is associated with poor prognosis and worse surviva PMID:21435435
Aberrant expression of TRIM24 negatively correlates with survival of breast cancer patients. PMID:21164480
TRIM24 regulates AR-mediated transcription in collaboration with TIP60 and BRD7. PMID:19909775
Preferential expression of the HTIF1alpha gene in acute myeloid leukemia and MDS-related AML. Could play a role in myeloid differentiation, being distinctly regulated in hematopoietic lineages. PMID:11986951
TIF1alpha interacts with TIF1gamma and the coiled-coil region of TIF1gamma is necessary for this interaction. PMID:12096914
We propose that ZCCHC11 is a unique TLR signal regulator, which interacts with TIFA after LPS treatment and suppresses the TRAF6-dependent activation of NF-kappaB. PMID:16643855
We identified a novel interaction between E4 ORF3 and TIF1alpha PMID:17287283

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Subcellular Location

Nucleus. Cytoplasm. Note=Colocalizes with sites of active transcription. Detected both in nucleus and cytoplasm in some breast cancer samples. Predominantly nuclear.

Database Links

HGNC: 11812

UNIGENE: Hs.490287

KEGG: hsa:8805

STRING: 9606.ENSP00000340507

OMIM: 603406

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