TRAF6 Recombinant Monoclonal Antibody

Code: CSB-RA037392A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, IHC, IF, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

Q9Y4K3

Target Names

TRAF6

Alternative Names

E3 ubiquitin-protein ligase TRAF6 antibody; Interleukin 1 signal transducer antibody; Interleukin-1 signal transducer antibody; MGC 3310 antibody; MGC:3310 antibody; MGC3310 antibody; OTTHUMP00000232772 antibody; OTTHUMP00000232773 antibody; RING finger protein 85 antibody; RNF 85 antibody; RNF85 antibody; TNF receptor associated factor 6 antibody; TNF receptor-associated factor 6 antibody; TNF receptor-associated factor 6; E3 ubiquitin protein ligase antibody; TRAF 6 antibody; Traf6 antibody; TRAF6_HUMAN antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human TRAF6 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

22D7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

IHC image of CSB-RA037392A0HU diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of Hela cell with CSB-RA037392A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA037392A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Description

TRAF6 serves as a critical signaling hub in innate immunity and inflammatory responses, functioning as an E3 ubiquitin-protein ligase that mediates signal transduction downstream of TNF receptors and interleukin-1 receptors. This adapter protein plays essential roles in NF-κB activation, making it a key target for researchers investigating inflammatory pathways, immune signaling cascades, and cancer biology.

This recombinant monoclonal antibody, clone 22D7, offers the consistency and reliability that demanding experimental workflows require. Because it is produced from a defined genetic sequence rather than traditional hybridoma methods, researchers benefit from lot-to-lot reproducibility that ensures comparable results across extended studies and collaborative projects. The rabbit IgG format provides excellent signal amplification while maintaining specificity for human TRAF6.

Validation across multiple detection platforms demonstrates this antibody's versatility in diverse experimental contexts. Immunohistochemistry studies in paraffin-embedded human pancreatic cancer tissue reveal clear cytoplasmic staining patterns at dilutions between 1:50 and 1:200, providing researchers flexibility in optimizing signal intensity for their specific tissue preparations. Immunofluorescence analysis in HeLa cells confirms robust detection of endogenous TRAF6 with distinct subcellular localization when counterstained with DAPI. Flow cytometry validation using SH-SY5Y neuroblastoma cells shows a clear positive shift compared to isotype controls, demonstrating suitability for quantitative single-cell analysis of TRAF6 expression levels.

The unconjugated format allows researchers to select secondary detection systems matched to their instrumentation and multiplexing requirements. Whether investigating TRAF6's role in inflammatory disease mechanisms, tumor microenvironment signaling, or fundamental immune cell biology, this antibody provides a dependable tool for advancing cell biology research.

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Target Background

Function(From Uniprot)

E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, IRAK1, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. Leads to the activation of NF-kappa-B and JUN. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B-lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor. Regulates osteoclast differentiation by mediating the activation of adapter protein complex 1 (AP-1) and NF-kappa-B, in response to RANK-L stimulation. Together with MAP3K8, mediates CD40 signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production.

Gene References into Functions

The overexpression of miR-146a significantly increased the ErbB4 expression, decreased the expression of TRAF6, IRAK1, caspase 3, and the phosphorylation level of NF-kappaB, and also increased the Bcl-2/Bax ratio, suggesting the inhibition of inflammation and apoptosis. PMID:30224945
TRAF6 can promote the proliferation of stromal cells of benign prostatic hyperplasia via Akt/mTOR signaling. PMID:29171041
Study identified IRAK1 and TRAF6 are direct targets of miR-146a in cervical cancer cells. Their expression is downregulated by miR-146a to promote cell viability. PMID:29693168
Immune profiling of human prostate epithelial cells in health and pathology determined by expression of p38/TRAF-6/ERK MAP kinases pathways has been reported. PMID:29475459
Downregulation of miR-124 in human osteosarcoma tissues and cell lines may increase the expression of TRAF6 protein. miR-124 may inhibit cell cycle progression and invasion of osteosarcoma cells, and promote apoptosis by regulating TRAF6. PMID:29436576
Low TRAF6 expression is associated with posterior longitudinal ligament ossification. PMID:29782494
these results suggest that miR-146a-5p affects proliferation and apoptosis in a cellular context-dependent manner and selectively disarms the TRAF6-mediated branch of the TGF-beta signaling in oral squamous cell carcinoma cell lines by sparing Smad4 involvement PMID:29048658
MALT1 and TRAF6 cooperatively interact with CARMA1-BCL10 filaments and form CARMA1-BCL10-MALT1-TRAF6 signalosome. PMID:29382759
our data demonstrated that miR146b5p, as a tumor suppressor, mediated temozolomide resistance in GBM cells through negatively regulating TRAF6 expression, indicating that miR146b5p and its targeted genes would be potential therapeutic targets for glioma therapy. PMID:29048680
genetic association studies in Han population in southern China: Data suggest that, in subjects with type 2 diabetes, an SNP in NLRX1 (rs4245191) is associated with macro-vascular complications and cerebral infarction in the population studied; no such association was found for two other SNPs in NLRX1 (rs10790286, rs561830) or for two SNPs in TRAF6 (rs5030445, rs16928973). (NLRX1 = NLR family member-X1) PMID:29046236
protein-protein interaction studies on TRAF6 and BSG to get molecular level insights of the reactions. PMID:28612997
miR-146a suppresses the inflammatory response in human white adipocytes via targeting the expression of IRAK1 and TRAF6. PMID:27922090
miRNA146a expression was significantly higher in hepatitis C patients with a best cut off value 1.63 to discriminate between hepatitis C patients and healthy controls. Meanwhile, it was negatively correlated to IRAK1 and TRAF6 levels and positively correlated to viral load in hepatitis C patients. PMID:28587864
Suggest role for EBV-EBER1 in the induction of increased TRAF6 expression in the peripheral B cells of Sjogren's patients. PMID:28470929
theses results indicate that miR-146b-5p inhibits tumor growth and metastasis of hepatocellular carcinoma by targeting TRAF6 mediated Akt phosphorylation PMID:28404923
TRAF6 plays direct roles in the pro-inflammatory effects and proliferation of Rheumatoid arthritis (RA) fibroblast-like synoviocytes. TRAF6 may serve as a potential treatment target in RA. PMID:28506571
MiR-146a declined, while TRAF6 increased in lupus nephritis patients compared with healthy controls. PMID:28549054
Epigallocatechin-3-gallate is a novel E3 ubiquitin ligase inhibitor that could be used to target TRAF6 activity in melanoma cells, inhibiting cell proliferation/migration. PMID:27791197
Results identified TRAF6 as a direct target of miR-429, and its downregulation partially attenuates the effect of miR-429 in promoting hepatocellular carcinoma cell proliferation and motility. PMID:28440423
Compared with normal chondrocytes, the expression of miR-146a decreased, while the mRNA and protein expressions of TRAF6 and NF-kappaB increased in osteoarthritis chondrocytes. PMID:28314786
We report two siblings with SCID and an atypical phenotype of osteopetrosis (OP). A biallelic microdeletion encompassing the 5' region of TRAF6, RAG1 and RAG2 genes was identified. TRAF6, a tumor necrosis factor receptor-associated family member, plays an important role in T cell signaling and in RANKL-dependent osteoclast differentiation and activation but its role in human OP has not been previously reported PMID:27808398
Taken together, these results define a novel role for miR-146a as a negative regulator of dengue virus-induced autophagy and identify TRAF6 as a key target of this microRNA in modulating the dengue virus-autophagy interaction. PMID:28825144
These data define that YOD1 antagonizes TRAF6/p62-dependent IL-1 signaling to NF-kappaB. PMID:28244869
high expression of TRAF6 is significant for esophageal cancer progression, and TRAF6 indicates poor prognosis in esophageal cancer patients. PMID:27697099
the polymorphisms in TLR-MyD88-NF-kappaB signaling pathway confer genetic susceptibility to Type 2 diabetes mellitus and diabetic nephropathy. PMID:27062898
The E3 ligase TRAF6 binds to DCP1a and indirectly regulates DCP1a phosphorylation, expression of decapping factors, and gene-specific mRNA decay. PMID:27315556
Study showed that patients without a history of atrial fibrillation who develop postoperative atrial fibrillation have a higher percentage of left atrial fibrosis, increased expression of TRAF6, higher serum Ang II levels, and changes in the activities of the MAPKs/TGF-beta1/TRAF6 pathway. PMID:28323847
Low TRAF6 expression is associated with graft-versus-host disease. PMID:27485827
Consistent with cellular studies, icaritin downregulated TRAF6 and NFATc1 protein expression in CD11b(+) /Gr-1(-/low) osteoclast precursors PMID:27813153
Results suggest that overexpression of miR-146a could promote IDD through the TRAF/NF-kappaB pathway. PMID:28161709
TRAF6 and H2AX overexpression and gammaH2AX-mediated HIF1alpha enrichment in the nucleus of cancer cells lead to overactivation of HIF1alpha-driven tumorigenesis, glycolysis and metastasis. PMID:27918549
These findings show that SopB suppresses host cell apoptosis by binding to TRAF6 and preventing mitochondrial reactive oxygen species generation. PMID:27473656
HCV infection suppressed the host innate immune response through the induction of autophagic degradation of TRAF6. This finding provided important information for further understanding how HCV evades host immunity to establish persistence PMID:27681126
Vav3 is a novel TRAF6 interaction partner that functions in the activation of cooperative signaling between T6BSs and the IVVY motif in the RANK signaling complex. PMID:27507811
TRAF6 expression was decreased in tympanic membrane of otitis media patients. PMID:27497395
Peripheral blood levels of S100A8 and TRAF6 in SAE patients were elevated and might be related to the severity of SAE and predict the outcome of SAE. PMID:27411454
Low TRAF6 expression is associated with Graves' disease. PMID:28219358
MiR-146a regulates inflammatory macrophage infiltration in Polymyositis/Dermatomyositis by targeting TRAF6 and affecting the IL-17/ICAM-1 pathway. PMID:27889748
Study demonstrates that TRAF6 is upregulated in human lung cancer cells, and siRNA-induced TRAF6 knockdown inhibits the invasion of lung cancer cells and promotes apoptosis. PMID:26847475
High TRAF6 expression is associated with melanoma invasion and metastasis. PMID:26769849
The expression of TRAF6 was positively correlated with an advanced N stage and acted as a predictor of a poor prognosis in patients with gastric cancer PMID:26627263
DAT stabilized IkBa by inhibiting the phosphorylation of Ika by the IkB kinase (IKK) complex. DAT induced proteasomal degradation of TRAF6, and DAT suppressed IKKb-phosphorylation through downregulation of TRAF6 PMID:26647777
TRAF6 may be involved in cell migration, invasion, and apoptosis of SPC-A-1 cells, possibly through regulating the NF-B-CD24/CXCR4 pathway.. PMID:26582220
In 816 matched pairs of Han Chinese with and without ischemic stroke, TRAF6 rs5030416 was associated with increased susceptibility in various genetic models. No association was found for rs5030411, although it was associated with total cholesterol. PMID:25999280
Identify miR-146b-5p as a tumor suppressor and novel prognostic biomarker of gliomas, and suggest miR-146b-5p and TRAF6 as potential therapeutic candidates for malignant gliomas. PMID:26320176
miR146a has an important promoting effect on the apoptosis of granulosa cells by targeting IRAK1 and TRAF6 via the caspase cascade pathway PMID:26151128
Findings show that TRAF6 is involved in regulating HUVECs proliferation after intermittent hypoxia by modulating cell signaling downstream of AT1R which can be helpful in treating cardiovascular disorders for instance. PMID:26223916
These findings suggest that TRAF6 is required for BLyS-mediated NF-kappaB signaling in myeloma cells PMID:26334396
TIFAB loss increases TRAF6 protein and the dynamic range of TLR4 signaling PMID:26458771
Transmembrane motif T6BM2-mediated TRAF6 binding is required for MAVS-related antiviral response. PMID:26385923