TP53BP2 Recombinant Monoclonal Antibody

Code: CSB-RA982352A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human, Mouse, Rat

Application: ELISA, WB, IHC, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

Q13625

Target Names

TP53BP2

Alternative Names

53BP2 antibody; Apoptosis stimulating of p53 protein 2 antibody; Apoptosis stimulating protein of p53 2 antibody; Apoptosis-stimulating of p53 protein 2 antibody; ASPP2 antibody; ASPP2_HUMAN antibody; BBP antibody; Bcl2 binding protein antibody; Bcl2-binding protein antibody; NY REN 51 antigen antibody; p53 binding protein 2 antibody; p53-binding protein 2 antibody; p53BP2 antibody; PPP1R13A antibody; Renal carcinoma antigen NY-REN-51 antibody; Tp53bp2 antibody; Tumor protein p53 binding protein 2 antibody; Tumor suppressor p53 binding protein 2 antibody; Tumor suppressor p53-binding protein 2 antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human TP53BP2 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

13E4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
, Positive WB detected in:Mouse brain tissue lysate(30µg), Rat brain lysate(30µg)
All lanes:TP53BP2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 126 kDa
Observed band size: 150 kDa
Exposure time：10s
IHC image of CSB-RA982352A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA982352A0HU diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing 786-O cells stained with CSB-RA982352A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Description

TP53BP2, also known as ASPP2, plays a critical role in apoptosis regulation by enhancing the tumor suppressor function of p53. This protein specifically stimulates the transactivation of pro-apoptotic genes by p53, making it an important target for researchers investigating cell death pathways, tumor suppression mechanisms, and cancer biology. Additionally, TP53BP2 interacts with Bcl-2 family proteins, positioning it at the intersection of multiple apoptotic signaling networks.

This recombinant monoclonal antibody, clone 13E4, offers the consistency and reproducibility that demanding experimental workflows require. Because recombinant antibodies are produced from defined sequences rather than traditional hybridoma methods, researchers benefit from lot-to-lot uniformity that supports reliable longitudinal studies and reproducible results across experiments. The rabbit IgG format and affinity-chromatography purification further ensure high specificity for your target.

Validation studies demonstrate robust performance across multiple applications and species. Western blot analysis of mouse and rat brain tissue lysates reveals strong detection at approximately 150 kDa, which exceeds the predicted molecular weight of 126 kDa. This size difference likely reflects post-translational modifications such as glycosylation or phosphorylation, which are common for proteins involved in signaling cascades. Immunohistochemistry validation in paraffin-embedded human brain tissue and human gastric cancer specimens confirms reliable tissue staining, while flow cytometry analysis using 786-O renal carcinoma cells demonstrates utility for single-cell analysis applications. The cross-species reactivity with human, mouse, and rat samples provides flexibility for comparative studies and translational research models.

Whether investigating p53-mediated apoptosis, characterizing tumor suppressor networks, or exploring TP53BP2 expression in disease contexts, this antibody delivers the performance and versatility needed for rigorous cell biology research.

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Target Background

Function(From Uniprot)

Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions with proteins such as TP53. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of NAE1 to conjugate NEDD8 to CUL1, and thereby decreases NAE1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42.

Gene References into Functions

Study have provided evidence that ASPP2 has an inhibitory influence on triplenegative breast cancer (TNBC) growth and metastasis, and that this may rely on using the PI3K/AKT pathway in a p53independent manner. PMID:29568874
the abnormal expression of ASPP2 may play an important role for development and metastasis in esophageal squamous cell carcinoma. PMID:29747775
These findings identify TP53BP2 as a strong candidate causative gene for central nervous system (CNS) defects in 1q41q42 microdeletion syndrome. PMID:27447114
Results showed that protein expression levels of ASPP2 and P53 were significantly higher in esophageal squamous cell carcinoma tissues than in paired noncancerous tissues. PMID:28103919
Expression levels of TP53BP2, FBXO28, and FAM53A genes were associated with patient survival specifically in ER-positive, TP53-mutated tumors. PMID:28179588
These data together implicated a critical impact of MiR-205/ASPP2 on promoting epithelial-mesenchymal transition. PMID:27929537
ASPP2 is a key regulator of BECN1-dependent autophagy. PMID:27929538
Our results suggest that Gal-1 and ASPP2 functionally compete in nanocluster for active Ras on the plasma membrane. ASPP2 dominates the biological outcome, thus switching from a Gal-1 supported growth-promoting setting to a senescence inducing and stemness suppressive program in cancer cells. Our results support Ras nanocluster as major integrators of tumour fate decision events. PMID:27437940
Downregulated expression of ASPP2 is associated with hepatocellular carcinoma. PMID:27473084
miR-548d-3p/TP53BP2 pathway is critically involved in the proliferation and apoptosis of breast cancer cells and may be new therapeutic target of breast cancer cells. PMID:26663100
ASPP1/2-PP1 complexes are required for chromosome segregation and kinetochore-microtubule attachments. PMID:26595804
ASSP2 expression is lower in cancerous tissues and decreased ASPP2 lead to higher cancer cells proliferation and autophagic flux, which contribute to the gemcitabine resistance. PMID:26438046
this study revealed a novel function of ASPP2 in modulating autophagy and apoptosis. PMID:25534115
ASPP2 may participate in the lipid metabolism of non-alcoholic steatohepatitis and attenuate liver failure. PMID:25256142
These results identify previously uncharacterized regions distributed throughout the protein sequence of ASPP2 as determinants of CagA binding, providing mechanistic insight into apoptosis reprogramming by CagA. PMID:25963096
ASPP2 directly induces the dephosphorylation and activation of junctional YAP PMID:25360797
CHOP is critical for mediating ASPP2-induced autophagic apoptosis by decreasing Bcl-2 expression and maintaining nuclear ASPP2-Bcl-2 complexes. PMID:25032846
ASPP1/2 interacted with centrosome linker protein C-Nap1. Co-depletion of ASPP1 and ASPP2 inhibited re-association of C-Nap1 with centrosome at the end of mitosis. PMID:25660448
these observations reveal that Itch and Yap1 have antagonistic roles in the regulation of ASPP2 protein stability through competing post-translational regulatory mechanism of ASPP2. PMID:25436413
phosphorylation of ASPP2 by RAS/MAPK pathway provides a novel link between RAS and p53 in regulating apoptosis PMID:24312625
Attenuated expression of apoptosis stimulating protein of p53-2 (ASPP2) in human acute leukemia is associated with therapy failure. PMID:24312201
higher rate Helicobacter pylori infection, an increased expression of inhibitor of apoptosis stimulating protein of p53 (iASPP), and decreased expression of apoptosis-stimulating of p53 protein 2(ASPP2) was present in gastric cancer PMID:23528480
our studies demonstrate the role of Siah2 in regulation of tight junction integrity and cell polarity under hypoxia, through its regulation of ASPP2 stability. PMID:23644657
Disruption of CagA and ASPP2 binding alters the function of ASPP2 and leads to the decreased survival of H. pylori-infected cells PMID:24474782
FIH-1 depletion did lead to impaired binding of Par-3 to ASPP2. PMID:23606740
We also unveiled a possible mechanistic link between ASPP2 and Csk/Src signaling pathway, implicating the multiple cellular functions of ASPP2. PMID:23671128
ASPP1 and ASPP2 cooperate with RAS to enhance p53-induced apoptosis suggests that loss of ASPP1 or ASPP2 expression may be a frequent event in human cancers with mutant RAS. PMID:23392125
When the Px(T)PxR motif is deleted or mutated via insertion of a phosphorylation site mimic (T311D), PP-1c fails to bind to all three ASPP proteins, ASPP1, ASPP2 and iASPP. PMID:23088536
ASPP2 binds to Ras-GTP at the plasma membrane and stimulates Ras-induced signaling and pERK1/2 levels via promoting Ras-GTP loading, B-Raf/C-Raf dimerization, and C-Raf phosphorylation. PMID:23248303
the mRNA expression of ASPP1 and ASPP2 was frequently dowregulated in tumor tissues, and this decreased significantly in samples expressing wild-type p53 PMID:22552744
Methylation in the promoter region of ASPP2 gene was not detected in lung cancer or adjacent non-neoplastic lung tissue. PMID:22169642
Helicobacter pylori cytotoxin-associated gene A (CagA) subverts the apoptosis-stimulating protein of p53 (ASPP2) tumor suppressor pathway of the host PMID:21562218
PP1A and ASPP2 play a critical role in promoting TAZ function by antagonizing the LATS kinase through TAZ dephosphorylation. PMID:21189257
study was to determine localization pattern of ASPP-2 in a variety of normal and malignant human tissues; study indicates that ASPP-2 has a specific distribution pattern within tissues and cells in a way that appears to be related to differentiation PMID:21183427
ASPP2(ANK-SH3) binds NFkappaB(p65) in a similar manner to its natural inhibitor IkappaB, suggesting a possible novel role for ASPP2 as an NFkappaB inhibitor PMID:19507243
ASPP1 and ASPP2 genes are frequently down-regulated by DNA methylation in HBV-positive hepatocellular carcinoma, which may play important roles in the development of HCC PMID:20034025
ASPP2 is a negative regulator of the neddylation pathway through specific interaction with APP-BP1 and suggest that dysfunction of the APP-BP1 interaction with APP may be one cause of Alzheimer's disease PMID:12694406
TP53BP2 encodes two mRNA species, either with (53BP2) or without exon 3 (ASPP2), by alternative splicing in various cell lines and tissues PMID:14766226
downstream of E2F, suggesting that it functions as a common link between the p53/p73 and Rb/E2F apoptotic pathways PMID:15592436
target of E2F transcription factor PMID:15731768
ASPP2 CpG island aberrant methylation could be one molecular and genetic alteration in wild-type p53 tumours. PMID:15757645
results suggest that the tumor protein p53 binding protein(TP53BP2) locus is associated with susceptibility to gastric cancer in the Korean population PMID:15986435
ASPP2/(53BP2L) expression regulation by proteasomal degradation modulates p53 apoptotic function PMID:16091363
In this study we explored the effect of NF-kappaB activation elicited by a physiological NF-kappaB inducer, interleukin-1beta (IL-1beta), and anti-apoptotic Bcl-2 family proteins on the 53BP2S-mediated apoptosis PMID:16098144
role of ASPP1, ASPP2, and iASPP as apoptotic specific regulators of p53 [review] PMID:16139958
suggest a possible role for the N-termini of ASPP proteins in binding to other proteins in the apoptotic response network PMID:17594908
53BP2S interacts and modulates the insulin signals mediated by insulin receptor substrates. PMID:17965023
identifies the interaction sites of Bcl-2 and its homologues with ASPP2 PMID:18719108
bcl2 -938C/C genotype has worse prognosis and lower survival in patients with renal cell carcinoma. In addition, the bcl2 -938C/A single nucleotide polymorphism was shown to be an independent adverse prognostic factor for renal cell carcinoma PMID:19539330

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Subcellular Location

Cytoplasm, perinuclear region. Nucleus. Note=Predominantly found in the perinuclear region. Some small fraction is nuclear. Sequester in the cytoplasm on overexpression of DDX42.

Protein Families

ASPP family

Tissue Specificity

Widely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocyte. Reduced expression in breast carcinomas expressing a wild-type TP53 protein. Overexpressed in lung cancer cell lines.

Database Links

HGNC: 12000

UNIGENE: Hs.523968

KEGG: hsa:7159

STRING: 9606.ENSP00000341957

OMIM: 602143

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