TNNT2 Recombinant Monoclonal Antibody

Code: CSB-RA058699A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, IHC, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

P45379

Target Names

TNNT2

Alternative Names

Troponin T, cardiac muscle (TnTc) (Cardiac muscle troponin T) (cTnT), TNNT2

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human TNNT2

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8H8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Immunohistochemistry (IHC) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

IHC image of CSB-RA058699A0HU diluted at 1:50 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.16% DAB.
IHC image of CSB-RA058699A0HU diluted at 1:50 and staining in paraffin-embedded human rectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.16% DAB.
Overlay Peak curve showing MCF-7 cells stained with CSB-RA058699A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

Cardiac troponin T, encoded by the TNNT2 gene, serves as a critical regulatory component of the troponin complex that controls calcium-mediated contraction in cardiac muscle. As a highly specific marker of cardiomyocyte identity and injury, TNNT2 has become indispensable in cardiovascular research, stem cell differentiation studies, and investigations into cardiac signaling pathways. Its expression patterns provide valuable insights into cardiomyocyte maturation, disease states, and regenerative medicine applications.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human TNNT2, offers the reproducibility and consistency that demanding research protocols require. The recombinant production method ensures sequence-defined specificity with minimal lot-to-lot variation, allowing you to maintain experimental continuity across long-term studies without the need to re-optimize conditions.

Validation testing demonstrates reliable performance across multiple experimental platforms. Immunohistochemistry on paraffin-embedded human heart tissue shows clear detection of TNNT2 at dilutions of 1:50, confirming utility for cardiac tissue analysis. Interestingly, staining in human rectal cancer tissue was also evaluated, providing researchers with comparative data for assessing cardiac marker expression in non-cardiac contexts. Flow cytometry validation using MCF-7 cells demonstrates effective intracellular detection following formaldehyde fixation and Triton X-100 permeabilization, with clear separation from isotype control, supporting applications in cell population analysis and differentiation studies.

The antibody's compatibility with ELISA, immunohistochemistry, and flow cytometry provides workflow flexibility for researchers investigating cardiac development, cardiomyocyte differentiation from pluripotent stem cells, or cardiac-specific protein expression in various experimental models. This versatility makes it a practical choice for cardiovascular biology and signal transduction research programs.

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Target Background

Function(From Uniprot)

Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Gene References into Functions

Troponin T is neither specific nor useful as cardiac biomarker for post mortem sample PMID:30173231
Cardiac troponin T is the key diagnostic marker in Non-ST elevated myocardial infarction (NSTEMI). PMID:29619842
the optimal cutoff-value of hs-TnT to improve diagnostic accuracy of acute myocardial infarction in chronic kidney disease patients, was determined. PMID:28145489
This study has confirmed the higher reference levels of hscTnT in neonates and young infants when compared with adult population PMID:29303610
The current results indicate that patients with nondipper hypertension (NDH) had higher high-sensitivity - cTnT levels than the patients with dipper hypertension. High-sensitivity -cTnT was an independent predictor of NDH and was significantly related with nondipping status. PMID:29278556
Data suggest that high sensitivity cardiac troponin (hs-cTnT) may help predict whether non-acute coronary syndrome (ACS) emergency department (ED) patients have a final non-ACS cardiac or non-cardiac/unknown origin diagnoses. PMID:28532247
Cardiac troponin T at the NMJ region contributes to NMJ functional decline with ageing mainly in the fast-twitch skeletal muscle through interfering with PKA signalling. PMID:28419739
On admission, high-sensitivity-troponin T/creatine kinase-MB ratio was significantly higher in takotsubo syndrome patients compared to myocardial infarction patients. PMID:28595746
Serum high-sensitivity cardiac troponin T (HS-cTnT) level is high and associated with carotid plaques in systemic lupus erythematous (SLE) patients. PMID:28610589
High sensitivity troponin T was detectable in over half of the congenitally corrected transposition of the great arteries population and was related to right ventricular function. PMID:28302319
In patients with perinatal asphyxia, the difference in troponin-T concentrations between moderate and severe encephalopathy was not statistically significant. Troponin-T concentration was significantly higher in babies with hypotensive shock and hepatic injury, but not acute kidney injury. PMID:28854510
Five of the 19 patients (26.3%) had either a pathogenic variant or a likely pathogenic variant in MYBPC3 (n=1), MYH7 (n=1), RYR2 (n=2), or TNNT2 (n=1). All five variants were missense variants that have been reported previously in patients with channelopathies or cardiomyopathies PMID:28202948
Plasma troponin T was not altered after stress in patients with a positive stress test or those with a negative stress test. PMID:27364992
IL-6 receptor blockade has an effect on high-sensitivity troponin T and NT-proBNP in rheumatoid arthritis PMID:27744141
An exercise-associated increase in hs-cTnT and NT-proBNP occurred in response to a 60-minute maximal swimming test that was independent of pubertal status/adolescent vs adults. The present data also suggests that baseline and postexercise hs-cTnT values are higher in male compared with female, with no sex differences in NT-proBNP values. PMID:29173327
Both continuous aerobic exercise and intermittent exercise results in increased serum levels of cardiac troponin T in sedentary men. PMID:28096042
Digenic inheritance of two novel variants in TNNT2 and MYH7 is associated with a severe form of dilated cardiomyopathy. PMID:28642161
Increased levels of preprocedural hs-cTnT are proportionally related to the risk of death and emerged as independent predictors of all-cause mortality within 1 year in stable coronary artery disease patients undergoing percutaneous coronary intervention. PMID:27296200
Among patients with chest pain and stable troponin levels, any detectable level of high-sensitivity cardiac troponin is associated with an increased risk of death and cardiovascular disease. PMID:29073949
High-sensitivity cTnT measurement increases the detection of perioperative myocardial infarction. PMID:28719430
In a heart failure cohort, high sensitivity troponin T serum levels had very significant prognostic value. PMID:27718443
Asymptomatic cTnT elevation might be strongly associated with all-cause and cardiovascular mortality in dialysis patients without diabetic nephropathy. PMID:27178276
Higher postoperative concentrations of high-sensitivity troponin T are independently associated with all-cause mortality in patients undergoing on-pump cardiac surgery. PMID:28537984
variants in TNNT2 and BAG3 are associated with a high propensity to life-threatening cardiomyopathy presenting from childhood and young adulthood. PMID:28669108
Binding of the MBNL zinc fingers to cardiac troponin T pre-mRNA is specific and relatively simple, unlike the complex multiple dimer-trimer stoichiometries postulated in some previous studies. PMID:28718627
The measurement of high-sensitivity cardiac troponin T plasma concentration within large nationally-representative surveys such as the Health Survey for England is feasible. PMID:28141863
Data suggest that the tropomyosin overlap region structure and function are affected differentially by a point mutation in cardiac tropomyosin (TPM1, D230N) that is associated with dilated cardiomyopathy as compared to a point mutation in cardiac troponin T (TNNT2, R92L) that is associated with hypertrophic cardiomyopathy. PMID:28603979
High T2-weighted signal intensity on cine MRI is associated with elevated troponin T in hypertrophic cardiomyopathy. PMID:27506680
The high negative predictive value of preoperative hs-cTnT and NT-proBNP suggest usefulness as a "rule-out" test to confirm low risk of postoperative Myocardial Infarction PMID:28002165
Generated is a gene-targeted knock-in murine model of the autosomal dominant Arg141Trp (R141W) mutation in human Tnnt2. Tnnt2 R141W mutation causes a Ca2+ desensitization and mice adapt by increasing Ca2+-transient amplitudes, which impairs Ca2+ handling dynamics, metabolism and responses to beta-adrenergic activation. PMID:27936050
A myosin activator improves actin assembly and sarcomere function of human-induced pluripotent stem cell-derived cardiomyocytes with a troponin T point mutation. PMID:27199119
Our study supports that mutations in MYH7 and MYBPC3 should be the first focus of moleculargenetic analysis in HCM, and that mutations in TNNT2 have a low prevalence in Brazilian population. All mutations detected were missense mutations, whereas two mutations in MYH7 had not been described before. PMID:27737317
Dyrk1A modulates SRp55-promoted cTnT exon 5 inclusion PMID:27049307
Our results suggest that multiple enzymes are involved in cTnT degradation, and that thrombin plays an important role. PMID:27816455
In patients with clinically stable angina pectoris, slightly elevated hs-cTnT levels may indicate the presence of complex coronary artery disease. PMID:25964074
TNNT2 gene mutation is associated with Early-Onset Hypertrophic Cardiomyopathy. PMID:27483260
The results showed that MYBPC3 25-bp deletion polymorphism was significantly associated with elevated risk of left ventricular dysfunction (LVD), while TTN 18 bp I/D, TNNT2 5 bp I/D and myospryn K2906N polymorphisms did not show any significant association with LVD. PMID:27350668
A novel heterozygous mutation (MYH7, p.Asn885Thr), and a variant of uncertain significance (TNNT2, p.Arg296His) were identified in 2 patients with familial hypertrophic cardiomyopathy. PMID:27082122
Discordant elevation of cTnT in the presence of normal CK plasma levels on admission is associated with increased mortality in STEMI patients undergoing primary PCI. PMID:26563201
High cardiac troponin T expression is associated with heart diseases. PMID:26975902
The results presented here have detected important prognostic differences between patients with 1 of the TNNT2 mutations (Arg92Gln) and those with the 5 other TNNT2 mutations. Carriers of the Arg92Gln mutation developed hypertrophic or dilated cardiomyopathy and had a significantly worse prognosis than those with other mutations in TNNT2. PMID:26507537
No adverse clinical events or functional changes of the device were noted, even in those with increases in cTnT. PMID:26489609
TNNT2 TNNT2 PMID:27363271
Segmental plaque volume and presence of virtual histology derived thin-cap fibroatheroma lesions are associated with higher circulating hsTnT concentrations in stable coronary artery disease patients. PMID:26917225
High-sensitivity cardiac troponin T could be used as an early biomarker for the risk stratification of patients with type-A acute aortic dissection. PMID:26916429
An association between elevated troponin levels and increased mortality was found in critically ill patients. PMID:26425879
For patients older than 65 years of age, applications of age-specific troponin T level cut-offs resulted in a decrease in acute myocardial infarction in the entire cohort. PMID:26878470
Data show that high-sensitivity cardiac troponin T (hs-cTnT) was detected in 244 (87.1%) type 2 diabetes mellitus patients. PMID:26823030
sensitivity and specificity of cardiac specific troponin T levels in detecting cardiac damage are much higher according to telecardiography and electrocardiography PMID:26720305
since hs-cTnT levels of non-AMI patients can be over the diagnostic cut-off value, further studies are necessary to define clinically applicable cut-off values of hs-cTnT PMID:26782442

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Protein Families

Troponin T family

Tissue Specificity

Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in

Database Links

HGNC: 11949

UNIGENE: Hs.533613

KEGG: hsa:7139

STRING: 9606.ENSP00000356286

OMIM: 115195

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