TAF3 Recombinant Monoclonal Antibody

Code: CSB-RA158092A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, WB, IHC, IF, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

Q5VWG9

Target Names

TAF3

Alternative Names

140 kDa TATA box-binding protein-associated factor antibody; Bip2 protein antibody; RNA polymerase II transcription factor TAFII140 antibody; TAF(II)140 antibody; TAF140 antibody; TAF3 antibody; TAF3 RNA polymerase II TATA box binding protein (TBP)-associated factor antibody; TAF3_HUMAN antibody; TAFII-140 antibody; TAFII140 antibody; TAFII155 protein antibody; TATA box binding protein associated factor 3 antibody; TBP-associated factor 3 antibody; Transcription initiation factor TFIID 140 kDa subunit antibody; Transcription initiation factor TFIID subunit 3 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human TAF3 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

12E12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: MCF-7 whole cell lysate(30µg), A549 whole cell lysate(30µg)
All lanes: TAF3 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 104 kDa
Observed band size: 150 kDa
Exposure time：5min
IHC image of CSB-RA158092A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of MCF-7 cell with CSB-RA158092A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing jurkat cells stained with CSB-RA158092A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Description

TAF3 serves as a critical component of the TFIID complex, functioning as a 140 kDa subunit essential for RNA polymerase II-mediated transcription initiation. This transcription factor plays a pivotal role in gene regulation by facilitating the assembly of the preinitiation complex at TATA box-containing promoters, making it a significant target for researchers investigating transcriptional control mechanisms and epigenetic regulation.

This recombinant monoclonal antibody, clone 12E12, offers the reproducibility and consistency that demanding experimental workflows require. Generated in rabbit against a synthetic peptide derived from human TAF3, the recombinant production method ensures sequence-defined specificity and eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations.

Extensive validation across multiple platforms demonstrates this antibody's versatility in your research toolkit. Western blot analysis in MCF-7 and A549 whole cell lysates reveals a band at approximately 150 kDa, which runs higher than the predicted 104 kDa molecular weight. This size difference likely reflects post-translational modifications, particularly glycosylation, which is common among nuclear proteins involved in transcriptional regulation. Immunohistochemistry validation in human colorectal cancer tissue sections confirms reliable detection in formalin-fixed, paraffin-embedded samples using standard citrate buffer antigen retrieval. Immunofluorescence studies in MCF-7 cells demonstrate clear nuclear localization patterns when counter-stained with DAPI, consistent with TAF3's role in transcription. Flow cytometry analysis in Jurkat cells further expands the application range for researchers studying TAF3 expression at the single-cell level.

This antibody supports investigations into transcriptional regulation, chromatin biology, and nuclear signaling pathways where understanding TFIID complex dynamics is essential.

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Target Background

Function(From Uniprot)

Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Required in complex with TBPL2 for the differentiation of myoblasts into myocytes. The complex replaces TFIID at specific promoters at an early stage in the differentiation process.

Gene References into Functions

Data show that transcription cofactor TAF3 is required for transcription of the alpha spectrin SPTA1 gene. PMID:23935956
Study shows that H3K4me3-TAF3 interactions direct global TFIID recruitment to active genes, some of which are p53 targets. Further analyses show that H3K4me3 enhances p53-dependent transcription by stimulating preinitiation complex formation. PMID:23452851
Study shows that this TAF3 activity is critical for early lineage segregation during stem cell differentiation. PMID:21884934
Data identified TAF3 as an evolutionarily conserved negative regulator of p53 transcription activation function. PMID:18549481