STAT5A/STAT5B Recombinant Monoclonal Antibody

STAT5A and STAT5B are closely related transcription factors that serve as critical downstream effectors of cytokine and growth factor signaling pathways. These proteins play essential roles in hematopoiesis, immune cell development, and mammary gland differentiation, while their dysregulation has been implicated in various malignancies, particularly leukemias and solid tumors. Understanding STAT5 activation and expression patterns remains central to research in immunology, oncology, and developmental biology.

This recombinant monoclonal antibody, generated from clone 3H9, offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant antibodies are produced from defined genetic sequences rather than traditional hybridoma methods, researchers benefit from lot-to-lot uniformity that supports longitudinal studies and ensures comparable results across experiments.

Validation studies demonstrate robust performance across multiple applications. In western blot analysis, this antibody detects STAT5A/B in a diverse panel of human cell lines including THP-1, A549, K-562, HeLa, and Jurkat cells, as well as mouse NIH/3T3 cells and mouse liver tissue, confirming reliable cross-species reactivity between human and mouse samples. The observed band at approximately 102 kDa runs slightly higher than the predicted 90 kDa molecular weight, which is consistent with post-translational modifications such as glycosylation that are characteristic of STAT5 proteins in their native cellular context. Immunohistochemistry validation in human lymph node tissue demonstrates clear staining with appropriate morphological detail, expanding this antibody's utility to tissue-based investigations.

Whether investigating JAK-STAT signaling dynamics, characterizing immune cell populations, or exploring STAT5-driven oncogenic programs, this antibody provides a dependable tool for researchers working across cell biology and translational applications.